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細胞色素C氧化酶亞基Ⅱ(COX2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)

ELISA Kit for Cytochrome C Oxidase Subunit II (COX2)

MTCO2; MT-CO2; COII; COXII; Mitochondrially Encoded Cytochrome C Oxidase II; Cytochrome c oxidase polypeptide II

  • 細胞色素C氧化酶亞基Ⅱ(COX2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 細胞色素C氧化酶亞基Ⅱ(COX2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 細胞色素C氧化酶亞基Ⅱ(COX2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) 實驗結果圖
  • SED284Ra.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測細胞色素C氧化酶亞基Ⅱ(COX2)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。

實驗原理

將細胞色素C氧化酶亞基Ⅱ(COX2)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的細胞色素C氧化酶亞基Ⅱ(COX2)與連接于固相載體上的抗體結合，然后加入生物素化的細胞色素C氧化酶亞基Ⅱ(COX2)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的細胞色素C氧化酶亞基Ⅱ(COX2)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。

相關產(chǎn)品

編號 適用物種：Rattus norvegicus (Rat，大鼠) 應用(僅供研究使用，不用于臨床診斷！)
RPD284Ra01 細胞色素C氧化酶亞基Ⅱ(COX2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAD284Ra01 細胞色素C氧化酶亞基Ⅱ(COX2)多克隆抗體 WB; IHC
SED284Ra 細胞色素C氧化酶亞基Ⅱ(COX2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD284Ra 細胞色素C氧化酶亞基Ⅱ(COX2)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
Radiation Research Molecular Mechanisms Underlying Chemopreventive Activities of Glycyrrhizic Acid against UVB- Radiation-Induced Carcinogenesis in SKH-1 Hairless Mouse Epidermis [PubMed: 21545294]
Photodermatology, Photoimmunology & Photomedicine Diallyl sulfide protects against ultraviolet B-induced skin cancers in SKH-1 hairless mouse: analysis of early molecular events in carcinogenesis [Wiley: source]
Journal of Drug Targeting Discovery?of a?novel?anti-cancer?agent?targeting?both?topoisomerase?I and?II?in?hepatocellular?carcinoma?Hep3B?cells?in?vitro?and in?vivo:?Cinnamomum?verum?component?2-methoxycinnamaldehyde. [Pubmed:26707867]
Drug Des Devel Ther Discovery of a novel anticancer agent with both anti-topoisomerase I and II activities in hepatocellular carcinoma SK-Hep-1 cells in vitro and in vivo: Cinnamomum verum component 2-methoxycinnamaldehyde [Pubmed:26792981]
Sci Total Environ. The?effects?of?particulate matter?on?inflammation?of?respiratory system:?Differences?betweenmale?and?female. [pubmed:28174048]
Frontiers in Pharmacology Docking Studies and Biological Evaluation of a Potential β-Secretase Inhibitor of 3-Hydroxyhericenone F from Hericium erinaceus. [pubmed:28553224]
Cytokine Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects [pubmed:29102164]
Cell Stress & Chaperones Effect of single and repeated heat stress on chemical signals of heat shock response cascade in the rat's heart. [pubmed:29178005]
Molecular Carcinogenesis Quercetin‐3‐methyl ether inhibits esophageal carcinogenesis by targeting the AKT/mTOR/p70S6K and MAPK pathways [Pubmed:30035335]
Neuroscience Celecoxib prevents cognitive impairment and neuroinflammation in soluble Amyloid β-treated rats [Pubmed:29306052]
Inflammopharmacology Polar extract of protects cartilage homeostasis: possible mechanism of action [Pubmed:29313174]
Regulatory Toxicology and Pharmacology Targeted inhibition of tumor survival, metastasis and angiogenesis by Acacia ferruginea mediated regulation of VEGF, inflammatory mediators, cytokine profile and?… [Pubmed:29678767]
Current Nutrition & Food Science Barrier Strengthening and Anti-inflammatory Effect of Cucurbit Fruits on Intestinal Epithelial Cells In-vitro [10.2174:1573401313666170427123153]
Metabolic?Brain?Disease Berberine ameliorates lipopolysaccharide-induced learning and memory deficit in the rat: insights into underlying molecular mechanisms [Pubmed: 30456649]
Metabolic?Brain?Disease Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanisms [Pubmed: 30421246]
Inflammopharmacology Chandrasekaran Chinampudur Velusami, Edwin Jothie Richard & []
Life?Sciences 1, 25-(OH) 2D3 protects Schwann cells against advanced glycation end products-induced apoptosis through PKA-NF-κB pathway [Pubmed: 30928409]
Biosci Biotechnol Biochem Polydatin ameliorates chemotherapy-induced cognitive impairment (chemobrain) by inhibiting oxidative stress, inflammatory response, and apoptosis in rats [Pubmed: 31992173]
Nutrients Dietary Supplementation of Foxtail Millet Ameliorates Colitis-Associated Colorectal Cancer in Mice via Activation of Gut Receptors and Suppression of the STAT3?… [Pubmed: 32784751]
Journal of Diabetes Research Regulation and Mechanism of miR-518d through the PPARα-Mediated NF-κB Pathway in the Development of Gestational Diabetes Mellitus [Pubmed: 33123597]
Journal of Information and Computational Science Rewiring of chemically modified MAPK pathway and its downstream targets by rosmarinic acid in mice dermal cancer []
Association between Serum Epidermal Growth Factor Receptor and Cyclooxygenase-2 Levels in Patients with Non-small Cell Carcinoma of Lung []
Biotechnol Rep Targeting NF-|êB mediated cell signaling pathway and inflammatory mediators by 1, 2-diazole in A549 cells in vitro [33598414]
Brain Sci Neuroprotective Effect of Clobenpropit against Lipopolysaccharide-Induced Cognitive Deficits via Attenuating Neuroinflammation and Enhancing Mitochondrial?… [34942919]
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