欧美综合自拍亚洲综合网_欧美黑人巨大videos异族_可乐操中文字幕_亚洲成av人福利专区网站_国产强奸视频在播放_免费无码国产在线53_最近最新的免費中文字幕_抖音免费视频素材_亚洲精品熟女一二区_国产无套粉嫩白浆在线麻豆

G蛋白偶聯(lián)雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for G Protein Coupled Estrogen Receptor 1 (GPER) ,etc. by FLIA (Flow Luminescence Immunoassay)

GPR30; CMKRL2; DRY12; FEG-1; GPCR-Br; LERGU; LyGPR; Membrane estrogen receptor; Chemoattractant receptor-like 2; Flow-induced endothelial G-protein coupled receptor 1

（注：單次混測多因子不超過8個指標(biāo) ）

  • G蛋白偶聯(lián)雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • G蛋白偶聯(lián)雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測G蛋白偶聯(lián)雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的G蛋白偶聯(lián)雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 80-94 86
EDTA plasma(n=5) 96-103 101
heparin plasma(n=5) 98-105 102
sodium citrate plasma(n=5) 83-95 87

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的G蛋白偶聯(lián)雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中G蛋白偶聯(lián)雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 87-101% 91-98% 79-98% 78-97%
EDTA plasma(n=5) 84-95% 81-102% 84-94% 78-103%
heparin plasma(n=5) 79-98% 98-105% 85-101% 80-103%
sodium citrate plasma(n=5) 94-101% 98-105% 98-105% 79-90%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。

實驗流程

1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
    37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將G蛋白偶聯(lián)雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的G蛋白偶聯(lián)雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的G蛋白偶聯(lián)雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的G蛋白偶聯(lián)雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈品

相關(guān)產(chǎn)品

編號 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPG045Hu01 G蛋白偶聯(lián)雌激素受體1(GPER)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAG045Hu01 G蛋白偶聯(lián)雌激素受體1(GPER)多克隆抗體 WB; IHC; ICC; IP.
MAG045Hu21 G蛋白偶聯(lián)雌激素受體1(GPER)單克隆抗體 WB; IHC; ICC; IP.
SEG045Hu G蛋白偶聯(lián)雌激素受體1(GPER)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMG045Hu G蛋白偶聯(lián)雌激素受體1(GPER)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
The Journal of Steroid Biochemistry and Molecular Biology Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR30 signalling [Pubmed:24846829]
Molecular and Cellular Endocrinology Isomer-nonspecific action of dichlorodiphenyltrichloroethane on aryl hydrocarbon receptor and G-protein-coupled receptor 30 intracellular signaling in apoptotic neuronal cells [Pubmed:24859647]
J Steroid Biochem Mol Biol Neuroprotective action of raloxifene against hypoxia-induced damage in mouse hippocampal cells depends on ERα but not ERβ or GPR32 signalling [PubMed: 24846831]
Journal of Environmental Sciences Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts [science:S1001074216300924]
Psychiatry research Serum levels of G protein-coupled estrogen receptor 1 (GPER1) in drug-naive patients with generalized anxiety disorder. [pubmed:27512921]
Clinical Psychopharmacology Neuroscience. Increased Serum G Protein-coupled Estrogen Receptor 1 Levels and Its Diagnostic Value in Drug Na?ve Patients with Major Depressive Disorder [pubmed:29073745]
Psychiatry?Research Decreased levels of G protein-coupled estrogen receptor in children with autism spectrum disorders. [pubmed:28734238]
Neuropsychiatric disease and treatment serum levels of gPer-1 in euthymic bipolar patients [Pubmed:29618927]
European archives of oto-rhino-laryngology An evaluation of G-protein coupled membrane estrogen receptor-1 level in stuttering [Pubmed:29299745]
Bosnian Journal of?Basic?Medical?Sciences Evaluation of estrogen and G protein-coupled estrogen receptor 1 (GPER) levels in drug-na?ve patients with attention deficit hyperactivity disorder (ADHD) [Pubmed:29659348]
Carleton University Research Virtual Environment In Vitro Study of Mechanisms Underlying the Developmental Effects of Bisphenol A Using Human Fetal Lung Fibroblasts []
Am J Otolaryngol GPER-1 and sex-hormone levels in patients with otosclerosis [Pubmed: 32144019]
Andrologia. Relationship between the G protein–coupled oestrogen receptor and spermatogenesis, and its correlation with male infertility [Pubmed: 32776559]
Journal of Radiation Research and Applied Sciences GPER-1 in chronic hepatitis B []
FASEB J Endocrine disruption in Crohn's disease: Bisphenol A enhances systemic inflammatory response in patients with gut barrier translocation of dysbiotic microbiota??- [34085740]
Comparing the Impact of Serum GPER-1 and Oxidant/Antioxidant Levels on Retinopathy in Diabetic Patients and Healthy Individuals: A Pilot Study []
Horm Mol Biol Clin Investig The change pattern in serum G protein-coupled estrogen receptor-1 (GPER1) levels during pregnancy with and without gestational diabetes mellitus [34787384]
留言咨詢