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補(bǔ)體成分5a(C5a)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Complement Component 5a (C5a)

  • 補(bǔ)體成分5a(C5a)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 補(bǔ)體成分5a(C5a)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對補(bǔ)體成分5a(C5a)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。

儲存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號 適用物種：Mus musculus (Mouse，小鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA388Mu01 補(bǔ)體成分5a(C5a)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA388Mu62 補(bǔ)體成分5a(C5a)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA388Mu61 補(bǔ)體成分5a(C5a)活性蛋白 Cell?culture;?Activity?Assays.
APA388Mu62 補(bǔ)體成分5a(C5a)活性蛋白 Cell?culture;?Activity?Assays.
EPA388Mu61 補(bǔ)體成分5a(C5a)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA388Mu01 補(bǔ)體成分5a(C5a)多克隆抗體 WB; IHC; ICC; IP.
LAA388Mu71 補(bǔ)體成分5a(C5a)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAA388Mu21 補(bǔ)體成分5a(C5a)單克隆抗體 WB; IHC; ICC; IP.
LAA388Mu72 補(bǔ)體成分5a(C5a)單克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
SEA388Mu 補(bǔ)體成分5a(C5a)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA388Mu 補(bǔ)體成分5a(C5a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA388Mu 補(bǔ)體成分5a(C5a)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA388Mu 補(bǔ)體成分5a(C5a)檢測試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA388Mu 補(bǔ)體成分5a(C5a)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
PSA388Mu01 補(bǔ)體成分5a(C5a)抗體對 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA388Mu01 補(bǔ)體成分5a(C5a)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Molecular Immunology Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitro [ScienceDirect: S0161589011008297]
Journal of Reproductive Immunology Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia [PubMed: 23415845]
The Journal of Clinical Investigation Complement component 5 contributes to poor disease outcome in humans and mice with pneumococcal meningitis [PubMed: PMC3195471]
Journal of Antimicrobial Chemotherapy Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model [Pubmed: 23645587]
Journal of Orthopaedic Research Conversion from external fixator to intramedullary nail causes a second hit and impairs fracture healing in a severe trauma model [Pubmed: 23070742]
SpringerPlus Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats [Pubmed: 24353977]
American Journal fo Reproductive Immunology Evaluation of the Markers of Inflammation in the Umbilical Cord Blood of Newborns of Mothers with Thrombophilia [Pubmed:25209155]
Gynecol Obstet Invest Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? [PubMed: 26485247]
Journal of Neuroimmunology Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis [Pubmed:27235358]
International Immunopharmacology Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery [Pubmed:26802602]
Digital Repository Dosagem de fra??es ativadas do sistema complemento em empiema induzido em ratos [10183]
Environmental Pollution Cadmium-induced immune abnormality is a key pathogenic event in human and rat models of preeclampsia [pubmed:27511439]
Cancer Letters Complement C5a/C5aR pathway potentiates the pathogenesis Q5 of gastric cancer by down-regulating p21 expression [pubmed:29031586]
Acta Paediatrica Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children [DOI:?10.1111/apa.14106]
Scientific Reports Splenectomy modulates early immuno-inflammatory responses to trauma-hemorrhage and protects mice against secondary sepsis [Pubmed: 30291296]
Nutrients The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes [Pubmed: 30736479]
Journal of?NeuroVirology Intrathecal complement activation by the classical pathway in tick-borne encephalitis [Pubmed: 30850976]
PLoS One Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model [Pubmed: 31247004]
Frontiers in Immunology C5a/C5aR Pathway is Critical for the Pathogenesis of Psoriasis [Pubmed: 31447855]
Phytomedicine Novel evidence that an alternative complement cascade pathway is involved in optimal mobilization of hematopoietic stem/progenitor cells in Nlrp3?… []
Aging C5aR deficiency attenuates the breast cancer development via the p38/p21 axis [Pubmed: 32669478]
STEM CELL REVIEWS AND REPORTS A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem … [Pubmed: 32406006]
Modulation of C5a-C5aR interactions against murine mammary cancer cell line []
FASEB J C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease [Pubmed:35503088]
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