血紅蛋白(HB)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Hemoglobin (HB)
Hgb; Haemoglobin; Heterotetramer(αβ)2
- 編號KSB409Mu01
- 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma and erythrocyte lysates
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.55-400ng/mL
- 可適靈敏度0.24ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 5400 ¥ 9000 ¥ 16200 ¥ 31500 ¥ 54000
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特異性
試劑盒中的抗體對血紅蛋白(HB)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Mus musculus (Mouse,小鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
NPB409Mu01 | 血紅蛋白(HB)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB409Mu01 | 血紅蛋白(HB)多克隆抗體 | WB; IHC; ICC; IP. |
LAB409Mu91 | 血紅蛋白(HB)多克隆抗體(辣根過氧化物酶標(biāo)記) | WB; IHC; ICC. |
SEB409Mu | 血紅蛋白(HB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB409Mu | 血紅蛋白(HB)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB409Mu01 | 血紅蛋白(HB)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology | Effects of ectoine on behavioural, physiological and biochemical parameters of Daphnia magna [Pubmed:25460046] |
PLoS One. | Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis [Pubmed:27027307] |
Parasites & Vectors | The intracellular bacterium Anaplasma phagocytophilum selectively manipulates the levels of vertebrate host proteins in the tick vector Ixodes scapularis. [pubmed:27561965] |
Proteomics. Clinical Applications | Elevated levels of protein AMBP in cerebrospinal fluid of women with preeclampsia compared to normotensive pregnant women. [pubmed:27615121] |
Molecular nutrition & food research | Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro [pubmed:28105758] |
Frontiers in Immunology | Oxidized Hemoglobin Is Antigenic and Immunogenic in Lupus [pubmed:28694810] |
新規(guī)人工酸素運搬體HemoAct を用いた脳虛血再灌流障害治療開発に関する研究 [:] | |
Acta?Neuropathologica Communications | Mural cell-derived laminin-α5 plays a detrimental role in ischemic stroke [Pubmed: 30777135] |
Angiogenesis | Decylubiquinone suppresses breast cancer growth and metastasis by inhibiting angiogenesis via the ROS/p53/BAI1 signaling pathway [Pubmed: 32020421] |
Food Bioscience | Water-extractable zinc protoporphyrin IX in Parma ham predominantly exists as complexes with hemoglobin and myoglobin [] |
Photochem Photobiol | Effects of Human Serum Albumin on the Fluorescence Intensity and Tumor Imaging Properties of IR‐780 Dye [34687567] |
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