層粘連蛋白(LN)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Laminin (LN)
LAM
- 編號(hào)KSA082Mu01
- 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.781-50ng/mL
- 可適靈敏度0.28ng/mL.
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 6221 ¥ 10368 ¥ 18662 ¥ 36288 ¥ 62208
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特異性
試劑盒中的抗體對(duì)層粘連蛋白(LN)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Mus musculus (Mouse,小鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
NPA082Mu01 | 層粘連蛋白(LN)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA082Mu01 | 層粘連蛋白(LN)多克隆抗體 | WB; IHC; ICC; IP. |
MEA082Mu | 層粘連蛋白(LN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEA082Mu | 層粘連蛋白(LN)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA082Mu | 層粘連蛋白(LN)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA082Mu | 層粘連蛋白(LN)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA082Mu01 | 層粘連蛋白(LN)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Stem Cell Research Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly [Pubmed: 24739658] |
PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592] |
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European Journal of Obstetrics & Gynecology and Reproductive Biology | Investigation?of?diagnostic?potentials?of?nine?different?biomarkers?in?endometriosis. [Pubmed:24813083] |
Archives of Plastic Surgery | Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas [PubMed: 26430624] |
Canadian Journal of Physiology and Pharmacology | Modified Citrus Pectin stops progression of liver fibrosis by inhibiting galactin-3 and inducing apoptosis of stellate cells [Doi: Abs] |
MOLECULAR AND CLINICAL ONCOLOGY | Clinical significance of serum laminin and typea??IV collagen levels in cutaneous melanoma patients [Pubmed:27330797] |
Am J Transplant. | Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation. [pubmed:27456745] |
Asian Pacific Journal of Cancer Prevention | Modulatory Effects of Chemoradiation on Angiogenic Factors and Laminin in Cervical Cancer: Link with Treatment Response [pubmed:29172262] |
Diabetes | Coordination Among Lipid Droplets, Peroxisomes and Mitochondria Regulates Energy Expenditure Through the CIDE-ATGL-PPARα Pathway in Adipocytes [Pubmed:29986925] |
European Food Research and Technology | Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis [10.1007:s00217-018-3119-z] |
Experimental Lung Research | High amplitude stretching of ATII cells and fibroblasts results in profibrotic effects [Pubmed: 31290711] |
Neurotherapeutics | Fabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve Repair [Pubmed: 31758411] |
Untersuchungen und semiquantitative Symmetrie-Analysen zur Extrazellul?rmatrix von Kalbs-Stimmlippen [] | |
ARCHIV?EUROMEDICA | CLINICAL SIGNIFICANCE OF LAMININ AND ELASTINE LEVELS IN CHILDREN WITH UNDIFFERENTIATED CONNECTIVE TISSUE DISEASE [] |
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