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降鈣素原(PCT)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Procalcitonin (PCT)

Pro-Calcitonin

  • 降鈣素原(PCT)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 降鈣素原(PCT)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)降鈣素原(PCT)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。

配套試劑

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編號(hào) 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
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參考文獻(xiàn)

雜志 參考文獻(xiàn)
Scandinavian Journal of Infectious Diseases Evaluation of neutrophilic CD64, interleukin 10 and procalcitonin as diagnostic markers of early- and late-onset neonatal sepsis [PubMed: 20085423]
International Journal of Radiation Oncology ? Biology ? Physics Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice [PubMed: 20637981]
Ulus Travma Acil Cerrahi Derg Diagnostic and prognostic value of procalcitonin and phosphorus in acute mesenteric ischemia [PubMed: 21935794]
Egyptian Journal of Bronchology Serum procalcitonin and its relation to the outcome of ventilator-associated pneumoniain children [Essbronchology: Source]
University of Waikato The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis [Waikato:Source]
Egyptian Journal of Medical Microbiology Evaluation of the Diagnostic Utility of Fc Gamma Receptor Type 1 (nCD64) and Procalcitonin in Comparison with Conventional Methods for Diagnosis of Early Onset Neonatal Sepsis [Ejmm:Source]
Nigerian Medical Journal Efficacy of measuring procalcitonin levels in determination of prognosis and early diagnosis of bacterial resistance in sepsis [Pubmed:25657488]
Wiley online library The use and value of procalcitonin in solid organ transplantation [PubMed: 25996831]
Balkan Med J Diagnostic Value of Procalcitonin Levels in Acute Mesenteric Ischemia [PubMed: 26185718]
The Journal of Clinical Endocrinology & Metabolism Macrocalcitonin is a novel pitfall in the routine of serum calcitonin immunoassay [PubMed: 26647152]
Journal of Human Hypertension Procalcitonin and Pentraxin-3: Current biomarkers in inflammation in white coat hypertension [PubMed: 26040439]
Medical Science Monitor Variation?of?Circulating?Inflammatory?Mediators?in?Staphylococcus?aureus?and?Escherichia?coli?BloodstreamInfection. [Pubmed:26772168]
International Immunopharmacology Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery [Pubmed:26802602]
Pancreatology. Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis. [Pubmed:26804005]
Acta?Medica Risk Factors?of?Acute?Pancreatitis?in?Oral?Double Balloon Enteroscopy. [pubmed:27638962]
The Clinical Respiratory Journal Changes in inflammatory mediators as a result of intermittent hypoxia in obstructive sleep apnea syndrome [pubmed:28960823]
SHOCK INTERLEUKIN-1 RECEPTOR 2: A NEW BIOMARKER FOR SEPSIS DIAGNOSIS AND GRAM-NEGATIVE/GRAM-POSITIVE BACTERIAL DIFFERENTIATION [pubmed:27984536]
Cellular?Physiology and Biochemistry Hepatic Macrophages are the Cell Source of Hepatic Procalcitonin in Acute Liver Failure [Pubmed:29913443]
Archives of Medical Science Inflammatory mediators in the diagnosis and treatment of acute pancreatitis: pentraxin-3, procalcitonin and myeloperoxidase [Pubmed:29593801]
Journal of Thoracic Disease Expression pattern of soluble triggering receptor expressed on myeloid cells-1 in mice with Acinetobacter baumannii colonization and infection in the lung [Pubmed:29707313]
Asian?Journal of Psychiatry Procalcitonin and C-reactive protein as peripheral inflammatory markers in antipsychotic drug-free schizophrenia patients [Pubmed:29723719]
European Review for Medical and Pharmacological Sciences The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia. [Pubmed: 31298341]
Medicina Modestly Elevated Serum Procalcitonin Levels in Patients with Rheumatoid Arthritis Free of Active Infection [Pubmed: 33080909]
MEDIATORS OF INFLAMMATION Reevaluation of Lung Injury in TNF-Induced Shock: The Role of the Acid Sphingomyelinase [Pubmed: 32410851]
Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes []
Journal of Immunology Research Moesin Is a Novel Biomarker of Endothelial Injury in Sepsis [33628853]
Journal of Medical Biochemistry Circulating nuclear factor-kappa B mediates cancer-associated inflammation in human breast and colon cancer [33776564]
Surg Endosc Original contribution: sleeve gastrectomy reduces soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) levels in patients with morbid obesity [Pubmed:35044516]
Bone Res Procalcitonin is expressed in osteoblasts and limits bone resorption through inhibition of macrophage migration during intermittent PTH treatment [Pubmed:35087025]
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