Ⅰ型膠原(COL1)多克隆抗體(生物素標(biāo)記)
Biotin-Linked Polyclonal Antibody to Collagen Type I (COL1)
- 編號(hào)LAA571Hu71
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 來(lái)源抗體標(biāo)記
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物生物素
- 原抗體 -
- 緩沖液成份磷酸鹽緩沖液(pH7.4,含有0.02% NaN3和50%甘油)
- 性狀液體
- 濃度500μg/mL
- 且適物種-
- 應(yīng)用WB; IHC; ICC.
如果抗體需用于流式細(xì)胞術(shù),請(qǐng)參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 623 ¥ 1453 ¥ 2076 ¥ 5190 ¥ 20760
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特異性
該抗體是針對(duì)COL1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別COL1。
用法
免疫印跡:0.2-2μg/mL;1:250-2500
免疫組織化學(xué):5-20μg/mL;1:25-100
免疫細(xì)胞化學(xué):5-20μg/mL;1:25-100
最佳稀釋倍數(shù)最終由用戶決定。
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
NPA571Hu01 | Ⅰ型膠原(COL1)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA571Hu01 | Ⅰ型膠原(COL1)多克隆抗體 | WB; IHC; ICC; IP. |
LAA571Hu71 | Ⅰ型膠原(COL1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAA571Hu21 | Ⅰ型膠原(COL1)單克隆抗體 | WB; IHC; ICC; IP. |
SEA571Hu | Ⅰ型膠原(COL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA571Hu | Ⅰ型膠原(COL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEA571Hu | Ⅰ型膠原(COL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
AEA571Hu | 抗Ⅰ型膠原抗體(Anti-COL1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Journal of hypertension | Urotensin II induces phenotypic differentiation, migration, and collagen synthesis of adventitial fibroblasts from rat aorta [PubMed: 18475149] |
Cardiovascular Research | Metformin attenuates cardiac fibrosis by inhibiting the TGFβ1–Smad3 signalling pathway [OxfordJournals: source] |
Acta Otolaryngol | Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa [PubMed: 19958244] |
Experimental Biology and Medicine | Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells [Rsmjournals: Source] |
Connect Tissue Res. | Role of moderate exercising on Achilles tendon collagen crimping patterns and proteoglycans [Pubmed: 23758268] |
Bulletin of Experimental Biology and Medicine | Modification of collagen formation by mesenchymal stem cells isolated from human adipose tissue in culture and after autotransplantation for abdominal hernia plasty [Pubmed: 24319714] |
Age( dordr). | In vitro tenocyte metabolism in aging and oestrogen deficiency [Springer: Source] |
PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
European Archives of Oto-Rhino-Laryngology | Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats [Pubmed: 24077847] |
Stem Cell Research Therapy | Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly [Pubmed: 24739658] |
Physiol. Res. | The unwounded skin remodeling in animal models of diabetes types 1 and 2. [Pubmed: 24020818] |
J Physiol Pharmacol. | Fibrogenic response of hepatic stellate cells in ovarectomised rats exposed to ketogenic diet [Pubmed: 23568967] |
22 | Combined effect of strontium and zoledronate on hydroxyapatite structure and bone cell responses [ScienceDirect: S0142961214003135] |
Journal of Clinical and Experimental Hepatology | Protective Effects of Norursodeoxycholic Acid Versus Ursodeoxycholic Acid on Thioacetamide-induced Rat Liver Fibrosis [ScienceDirect: S0973688314000073] |
Human Molecular Genetics Online | Dystrophic mdx mice develop severe cardiac and respiratory dysfunction following genetic ablation of the anti-inflammatory cytokine IL-10 [Pubmed:24659498] |
PLoS One. | Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592] |
Journal of Biomedical Materials Research Part A | Carboxymethyl cellulose—hydroxyapatite hybrid hydrogel as a composite material for bone tissue engineering applications [Pubmed:23720392] |
Experimental Biology adn Medicine | Endostar, a novel human recombinant endostatin, attenuates liver fibrosis in CCl4-induced mice [Pubmed:24872431] |
International Journal of Clinical & Experimental Pathology | Mast cell chymase in keloid induces profibrotic response via transforming growth factor-β1/Smad activation in keloid fibroblasts [Pubmed:25120737] |
FEBS Letters | Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells [Pubmed:25542352] |
Cardiology | KLF15 is an essential negative regulatory factor for the cardiac remodeling response to pressure overload [Pubmed:25633973] |
PLOS ONE | Impact of the Uremic Milieu on the Osteogenic Potential of Mesenchymal Stem Cells [Pubmed:25635832] |
Journal of Colloid and Interface Science | Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses [Pubmed:25706198] |
Macromolecular Bioscience | Multi‐Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co‐Cultured Human Mesenchymal Stem Cells [PubMed: 26126665] |
journal of hard Tissue Biology | Biological Evaluation of a Prototype Material made of Polyglycolic Acid and Hydroxyapatite [Article: Jhtb] |
Mol Med Rep | NIM811 downregulates transforming growth factor [PubMed: 26573209] |
Front Pharmacol | Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats [PubMed: 26483688] |
Chemosphere | Effects of fluoride on the ultrastructure and expression of Type I collagen in rat hard tissue [PubMed: 25655816] |
J Surg Res | Tanshinone IIA ameliorates bleomycin-induced pulmonary fibrosis and inhibits transforming growth factor-beta-β–dependent epithelial to mesenchymal transition [PubMed: 25911951] |
Molecular and Cellular Biochemistry | SVVYGLR motif of the thrombin-cleaved N-terminal osteopontin fragment enhances the synthesis of collagen type III in myocardial fibrosis [PubMed: 26112906] |
Biointerphases | Incorporation of nanostructured hydroxyapatite and poly(N-isopropylacrylamide) in demineralized bone matrix enhances osteoblast and human mesenchymal stem cell activity [PubMed: 26443012] |
Gels | On the Mechanism of Drug Release from Polysaccharide Hydrogels Cross-Linked with Magnetite Nanoparticles by Applying Alternating Magnetic Fields: the Case of DOXO Delivery [2310-2861: 1] |
Acta Biomater | Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study [PubMed: 26689470] |
Int J Clin Exp Med | Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-β1 with concomitant induce Smad7 in human hypertrophic scar … [PubMed: 26309506] |
International Journal of Molecular Sciences | Hybrid Macro-Porous Titanium Ornamented by Degradable 3D Gel/nHA Micro-Scaffolds for Bone Tissue Regeneration [Pubmed:27092492] |
中國(guó)眼耳鼻喉科雜志 | 白細(xì)胞介素10 上調(diào)表達(dá)對(duì)家兔慢性細(xì)菌性鼻竇炎黏膜創(chuàng)傷修復(fù)的影響 [article:11593] |
Acta Biomaterialia | Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study [Pubmed:26689470] |
scientific reports | IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy [Pubmed:27263531] |
Cell Mol Immunol? | Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica [Pubmed:27157496] |
Кард?олог?я | ?нфаркт м?окарда, ускладнений аневризмою л?вого шлуночка: кл?н?чн? ? структурно-функц?ональн? особливост? переб?гу, д?агностичн?, терапевтичн? та прогностичн? аспекти [bitstream:123456789] |
Inflammation Research | Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy [pubmed:27516211] |
Indian Journal of Pharmaceutical Education and Research | Inhibition of hesperidin on epithelial to mesenchymal transition of non-small cell lung cancer cells induced by TGF-β1 [sites:default] |
Heart & Vessels | Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [pubmed:28004175] |
biochemical and biophysical research communications | Positive feedback loop of YB-1 interacting with Smad2 promotes liver fibrosis. [pubmed:28153731] |
Frontiers in Pharmacology | Induced pluripotent stem cells inhibit bleomycin-induced pulmonary fibrosis in mice through suppressing TGF-β1/Smad-mediated epithelial to mesenchymal … [PMC5108931] |
International?Journal?of?Molecular?Sciences | Plasma?LncRNA-ATB, a?Potential?Biomarker?for?Diagnosis?of?Patients?with?Coal?Workers'Pneumoconiosis: A?Case-Control?Study. [pubmed:27556453] |
BMC Cancer | Saikosaponin?d?induces?cell?death?through?caspase-3-dependent,?caspase-3-independent?andmitochondrial?pathways?in?mammalian?hepatic?stellate?cells. [pubmed:27461108] |
J Cell Physiol. | An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells. [pubmed:28240358] |
Basic Clin Pharmacol Toxicol. | Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. [pubmed:28032440] |
Acta Biomater. | Antiresorptive and anti-angiogenetic octacalcium phosphate functionalized with bisphosphonates: An in vitro tri-culture study. [pubmed:28238916] |
The American Journal of Chinese Medicine | Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cells [pubmed:28231747] |
Biomedical and Environmental Sciences | Crystalline Silica Promotes Rat Fibrocyte Differentiation?in Vitro, and Fibrocytes Participate in Silicosis?in Vivo [pubmed:29081339] |
Basic & Clinical Pharmacology & Toxicology | Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling [doi:10.1111] |
Dental Materials Journal | Involvement of the calcium-sensing receptor in mineral trioxide aggregate-induced osteogenic gene expression in murine MC3T3-E1 cells. [pubmed:28228629] |
Biomedicine & pharmacotherapy | Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. [pubmed:28742995] |
Journal of Applied Biomaterials & Functional Materials | Demineralized dentin and enamel matrices as suitable substrates for bone regeneration [pubmed:28731486] |
Acta Biomaterialia | Selective effect of hydroxyapatite nanoparticles on osteoporotic and healthy bone formation correlates with intracellular calcium homeostasis regulation [pubmed:28698163] |
Advanced Healthcare Materials | Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events. [pubmed:29120858] |
Macromolecular?Bioscience | Strontium‐Substituted Hydroxyapatite‐Gelatin Biomimetic Scaffolds Modulate Bone Cell Response [Pubmed:29877029] |
International Journal of Molecular Medicine | Adenovirus?mediated knockdown of activin A receptor type 2A attenuates immune?induced hepatic fibrosis in mice and inhibits interleukin?17?induced?… [Pubmed:29620144] |
PLoS One | Effect of hyperbaric oxygen therapy (HBO) on implant-associated osteitis in a femur fracture model in mice [Pubmed:29377928] |
Royal?Society?of?Chemistry | In vitro and in vivo assessment of nanostructured porous biphasic calcium phosphate ceramics for promoting osteogenesis in an osteoporotic environment [c8ra00768c] |
European Food Research and Technology | Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis [10.1007:s00217-018-3119-z] |
Journal of?Applied?Biomaterials?&?Functional?Materials | BMP-2 and type I collagen preservation in human deciduous teeth after demineralization [Pubmed:30045659] |
Theriogenology | Effect of transforming growth factor-β1 on α-smooth muscle actin and collagen expression in equine endometrial fibroblasts [Pubmed: 30321755] |
ACS?Applied?Materials?&?Interfaces | Fabrication and characterization of core-shell nanofibers using a next-generation airbrush for biomedical applications [Pubmed: 30433758] |
Chemico-Biological?Interactions | Lead exposure inhibits osteoblastic differentiation and inactivates the canonical Wnt signal and recovery by icaritin in MC3T3-E1 subclone 14 cells [Pubmed: 30731080] |
Oxidative Medicine and Cellular Longevity | Effects of the (Pro) renin Receptor on Cardiac Remodeling and Function in a Rat Alcoholic Cardiomyopathy Model via the PRR-ERK1/2-NOX4 Pathway [] |
Tropical Journal of Pharmaceutical Research | Methylbenzoxime as a therapeutic agent for glucocorticoid-induced osteoporosis in rats. [] |
Scientific Reports | Low fibrosis biomarker levels predict cardiac resynchronization therapy response [Pubmed: 30988339] |
Experimental Lung Research | High amplitude stretching of ATII cells and fibroblasts results in profibrotic effects [Pubmed: 31290711] |
BMC Nephrology | Serum sclerostin levels are positively related to bone mineral density in peritoneal dialysis patients: a cross-sectional study [Pubmed: 31315601] |
Archives of Dermatological Research | Extracellular matrix deposition by adipose-derived stem cells and fibroblasts: a comparative study [Pubmed: 31616972] |
Drug Delivery and Translational Research | A multifunctional dexamethasone-delivery implant fabricated using atmospheric plasma and its effects on apoptosis, osteogenesis and inflammation [Pubmed: 31898081] |
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Pantoea agglomerans chronic exposure induces epithelial-mesenchymal transition in human lung epithelial cells and mice lungs [Pubmed: 32146192] |
Molecular Metabolism | SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α [Pubmed: 32029232] |
ACS Applied Materials & Interfaces | ZIF-8 Modified Multifunctional Bone-Adhesive Hydrogels Promoting Angiogenesis and Osteogenesis for Bone Regeneration [Pubmed: 32814397] |
in vitro cellular & developmental biology-animal | Distinct differences in hypoxic responses between human oral mucosa and skin fibroblasts in a 3D collagen matrix [Pubmed: 32588253] |
Molecular Medicine | Circulating fibrocytes traffic to the lung in murine acute lung injury and predict outcomes in human acute respiratory distress syndrome: a pilot study [Pubmed: 32460694] |
Predictors of myocardial fibrosis and loss of epicardial adipose tissue volume in the long-term period after myocardial infarction [] | |
ACS Biomaterials Science & Engineering | Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction [] |
Ассоциации толщины эпикардиального жира и циркулирующих маркеров фиброза миокарда у пациентов с инфарктом миокарда [] | |
Ann Med Surg | Trauma magnitude of the meta-epyphyseal cancellous affects the incidence of deep vein thrombosis. A prospective cohort study on the dynamic of collagen I ?- [33717478] |
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | Uncarboxylated osteocalcin reverses the high glucose?induced inhibition of the osteogenic differentiation of MC3T3E1 cells via the GPRC6A/cAMP/PKA/AMPK signaling pathway [33786607] |
PLoS One | Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies [33999928] |
Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells [] | |
Russian Journal of Cardiology | Early diagnosis of myocardial fibrosis in patients with epicardial obesity [] |
Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction [] | |
Parasit Vectors | Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate?… [34857049] |
Pharmacol Res | Esomeprazole Alleviates Fibrosis in Systemic Sclerosis by Modulating AhR/Smad2/3 Signaling [Pubmed:34995795] |
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