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α-生育酚(TCPa)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Alpha-Tocopherol (TCPa)

VE; Vitamin E; α-TCP; α-Tocopherol

  • α-生育酚(TCPa)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • α-生育酚(TCPa)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)α-生育酚(TCPa)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過(guò)夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開(kāi)封,在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào) 適用物種:Pan-species (General,通用) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
CPA922Ge11 α-生育酚(TCPa)牛血清白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
CPA922Ge21 α-生育酚(TCPa)卵白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
PAA922Ge01 α-生育酚(TCPa)多克隆抗體 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA922Ge71 α-生育酚(TCPa)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
CEA922Ge α-生育酚(TCPa)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA922Ge α-生育酚(TCPa)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA922Ge α-生育酚(TCPa)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA922Ge11 α-生育酚(TCPa)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
International Journal of Advanced Research Evaluation of enzymatic and non- enzymatic antioxidant status in seminal plasma of Iraqi Infertile Men [Com:Source]
Journal of Physiology and Pharmacology Effect of vitamin E on cerebral cortical oxidative stress and brain-derived neurotrophic factor gene expression induced by hypoxia and exercise in rats. [PubMed: 25903950]
Mycotoxin Res? The?potential?effects?of?antioxidant?feed?additives?in?mitigating?the?adverse?effects?of?cornnaturally?contaminated?with?Fusarium?mycotoxins?on?antioxidant?systems?in the intestinal mucosa, plasma, and liver in weaned pigs. [Pubmed:27021614]
The Journal of Nutritional Biochemistry Vitamin E and caloric restriction promote hepatic homeostasis through expression of connexin 26, N-cad, E-cad and cholesterol metabolism genes [pubmed:27816814]
Plant Physiol Biochem Ultrasonication of in vitro potato single node explants: Activation and recovery of antioxidant defence system and growth responses [pubmed:29102903]
PLoS One Oxidative stress in lung cancer patients is associated with altered serum markers of lipid metabolism [Pubmed: 30973911]
Prooxidant effect of α-tocopherol in pancreatic tumors. []
Vitamin A, C, D, E and B12 Levels in Leprosy: A Case Control Study []
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