雄烯二酮(ASD)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Androstenedione (ASD)
4-Androstenedione; 4-Androstene-3,17-Dione
- 編號KSA456Ge11
- 物種Pan-species (General,通用) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競爭抑制酶聯免疫吸附試驗法測抗原
- 可檢測范圍123.5-10000pg/mL
- 可適靈敏度48.8pg/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 8597 ¥ 14328 ¥ 25790 ¥ 50148 ¥ 85968
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特異性
試劑盒中的抗體對雄烯二酮(ASD)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Pan-species (General,通用) | 應用(僅供研究使用,不用于臨床診斷!) |
CPA456Ge11 | 雄烯二酮(ASD)牛血清白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
CPA456Ge21 | 雄烯二酮(ASD)卵白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
PAA456Ge01 | 雄烯二酮(ASD)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
LAA456Ge71 | 雄烯二酮(ASD)多克隆抗體(生物素標記) | WB; IHC; ICC. |
MAA456Ge21 | 雄烯二酮(ASD)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA456Ge22 | 雄烯二酮(ASD)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
CEA456Ge | 雄烯二酮(ASD)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
IEA456Ge | 雄烯二酮(ASD)檢測試劑盒(酶聯免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA456Ge | 雄烯二酮(ASD)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA456Ge11 | 雄烯二酮(ASD)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Plos One | Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota [Pubmed:27093642] |
Scientific Reports | Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus [pubmed:27461373] |
33 | Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. [pubmed:28336389] |
Journal of Endocrinology | Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome [Pubmed: 29535146] |
International Journal of Biological Sciences | Melatonin Stimulates STAR Expression and Progesterone Production via Activation of the PI3K/AKT Pathway in Bovine Theca Cells [] |
Molecular reproduction and development | Evidence that downregulation of Wilms' tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries [Pubmed: 31490589] |
DOMESTIC ANIMAL ENDOCRINOLOGY | Wilms' tumor (WT1)(+/-KTS) variants decreases the progesterone secretion of bovine ovarian theca cells [Pubmed: 32739762] |
ANIMAL REPRODUCTION SCIENCE | A study on steroidogenic elaborations of stroma and their regulation in response to ovarian hormones in goats [33845412] |
Reprod Domest Anim | Extracellular Vesicles of Bovine Small Follicular Fluid Promote Ovarian Cortical Stromal Cell Proliferation and Steroidogenesis [34402549] |
Curr Oncol | Plasma Aromatase Activity Index, Gonadotropins and Estrone Are Associated with Frailty Syndrome in Post-Menopausal Women with Breast Cancer [Pubmed:35323344] |
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