血管緊張素Ⅱ(AngII)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Angiotensin II (AngII)
Ang-II; Angiotensin-2
- 編號KSA005Ra11
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍24.69-2000pg/mL
- 可適靈敏度9.22pg/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對血管緊張素Ⅱ(AngII)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| MAA005Ra21 | 血管緊張素Ⅱ(AngII)單克隆抗體 | WB; IHC; ICC; IP. |
| CEA005Ra | 血管緊張素Ⅱ(AngII)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA005Ra | 血管緊張素Ⅱ(AngII)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA005Ra11 | 血管緊張素Ⅱ(AngII)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Nature Medicine | Angiotensin-Converting Enzyme 2 Overexpression Remarkably Ameliorated Glomerular Injury in a Rat Model of Diabetic Nephropathy: A Comparison with ACE Inhibition [MolMed: 10_11_liu] |
| The European Journal of Clinical Investigation | Mesangial medium with IgA1 from IgA nephropathy inhibits nephrin expression in mouse podocytes [Wiley: source] |
| Immunopharmacology and Inflammation | Evidence for the complementary and synergistic effects of the three-alkaloid combination regimen containing berberine, hypaconitine and skimmianine on the ulcerative colitis rats induced by trinitrobenzene-sulfonic acid [ScienceDirect: S0188440910001049] |
| Nephrology | Effect of aggregated immunoglobulin A1 from immunoglobulin A nephropathy patients on nephrin expression in podocytes [Wiley: source] |
| PLoS One | Angiotensin II and Angiotensin-(1-7) in Paraventricular Nucleus Modulate Cardiac Sympathetic Afferent Reflex in Renovascular Hypertensive Rats [PubMed: PMC3527547] |
| BioMed Research International | Heterogeneous Downregulation of Angiotensin II AT1-A and AT1-B Receptors in Arterioles in STZ-Induced Diabetic Rat Kidneys [Hindawi: 947506] |
| 34 | Biglcan is beneficial in angiotensin II induced heart failure by preventing cardiac inflammation and remodeling improving LV function and mortality by preventing transdifferentiation of myofibroblasts [Oxfordjournals: Source] |
| Pflügers Archiv-European Journal of Physiology | SOD1 gene transfer into paraventricular nucleus attenuates hypertension and sympathetic activity in spontaneously hypertensive rats [Pubmed: 23114721] |
| Eur J Pharmacol. | Angiotensin II and vasopressin are involved in the defense system against anaphylactic hypotension in anesthetized rats [Pubmed:24650734] |
| J Mol Cell Cardiol. | Increased LDL electronegativity in chronic kidney disease disrupts calcium homeostasis resulting in cardiac dysfunction [Pubmed:25871829] |
| Mol Med Rep. | High glucose induces activation of the local renin?angiotensin system in glomerular endothelial cells [Pubmed:24337709] |
| Mol Med Rep. | Angiotensin II promotes the progression of human gastric cancer [Pubmed:24424956] |
| PLOS ONE | Intermedin in the Paraventricular Nucleus Attenuates Cardiac Sympathetic Afferent Reflex in Chronic Heart Failure Rats [Pubmed:24709972] |
| Journal of Vascular Surgery | Cathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice [Pubmed:25037606] |
| Biochim Biophys Acta. | Cathepsin G activity lowers plasma LDL and reduces atherosclerosis [Pubmed:25092171] |
| Acta Physiologica | Superoxide anions in the paraventricular nucleus mediate cardiac sympathetic afferent reflex in insulin resistance rats [Pubmed:25307720] |
| Neuroscience Letters | Administration of exogenous 1,25(OH)2D3 normalizes overactivation of the central renin-angiotensin system in 1α(OH)ase knockout mice [Pubmed:25576706] |
| 34 | A background Ca2+?entry pathway mediated by TRPC1/TRPC4 is critical for development of pathological cardiac remodelling [PubMed: 26069213] |
| Life Sci | Cardioprotective effects of baicalein on heart failure via modulation of Ca2 [PubMed: 26706290] |
| Physiological Research | Renal Sympathetic Denervation Improves Cardiac Dysfunction [PubMed: 25804096] |
| Nutrition | Effects of Rubus occidentalis extract on blood pressure in subjects with prehypertension: randomized, double-blinded, placebo controlled clinical trial [PubMed: 26740254] |
| J Am Soc Hypertens | Angiotensin-(1–7) enhances the effects of angiotensin II on the cardiac sympathetic afferent reflex and sympathetic activity in rostral ventrolateral medulla in renovascular hypertensive rats [PubMed: 26428223] |
| PLoS One. | Up ReCavia (Guinea pig )lation of cystathione γ lyase and Hydrogen Sulphide in the Myocardium Inhibits the Progression of Isoproterenol–Caffeine Induced Left Ventricular Hypertrophy in Wistar Kyoto Rats [Pubmed:26963622] |
| Nutrition | Effects of Rubus occidentalis extract on blood pressure in patients with prehypertension: Randomized, double-blinded, placebo-controlled clinical trial [Pubmed:26740254] |
| Journal of Atherosclerosis and Thrombosis | Involvement of Angiotensin II Type 1 and 2 Receptors in Gelatinase ReCavia (Guinea pig )lation in Human Carotid Atheroma in vitro [Pubmed:26947595] |
| Mol Neurobiol | A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats [Pubmed:26867655] |
| Life Sci | Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitro [Pubmed:26706290] |
| PLoS One. | Paradoxical Sleep Deprivation Causes Cardiac Dysfunction and the Impairment Is Attenuated by Resistance Training. [pubmed:27880816] |
| Oxidative Medicine and Cellular Longevity | Epigallocatechin-3-gallate Attenuates Renal Damage by Suppressing Oxidative Stress in Diabetic db/db Mice [pubmed:27698952] |
| Journal of the Renin-Angiotensin-Aldosterone System | Angiotensin type 2 receptor null mice express reduced levels of renal angiotensin converting enzyme-2/angiotensin (1-7)/Mas receptor and exhibit greater high-fat diet-induced kidney injury [1470320316661871.abstract] |
| Microvascular?Research | Pleiotropic?effects?of?survivin?in?vascular endothelial cells. [pubmed:27373518] |
| PLOS?ONE | Reduced?Expression?of the?Extracellular?Calcium-Sensing?Receptor?(CaSR) Is?Associated?withActivation?of the?Renin-Angiotensin?System?(RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension. [pubmed:27391973] |
| journal of pharmacological sciences | β-Aminoisobutyric?acid?ameliorates?the?renal?fibrosis?in?mouse?obstructed?kidneys?via?inhibitionof?renal?fibroblast?activation?and?fibrosis. [pubmed:28433566] |
| Experimental?Animals | Ginsenoside?Rb1?improves?cardiac?function?and?remodeling?in?heart?failure. [pubmed:28367863] |
| Oncotarget | Effects of renal denervation on monocrotaline induced pulmonary remodeling [pubmed:28187460] |
| The American Journal of Pathology | The Renin-Angiotensin System Regulates Neurodegeneration in a Mouse Model of Optic Neuritis [pubmed:28919108] |
| PLoS One | Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy [pubmed:29240788] |
| Frontiers in Physiology | Hydrogen Sulfide Attenuates Atherosclerosis in a Partially Ligated Carotid Artery Mouse model via Regulating Angiotensin Converting Enzyme 2 Expression [pubmed:29066981] |
| Neurotherapeutics | Brain Renin–Angiotensin System Blockade Attenuates Methamphetamine-Induced Hyperlocomotion and Neurotoxicity [Pubmed:29464572] |
| International Journal of Cardiology | ALDH2 protects against alcoholic cardiomyopathy through a mechanism involving the p38 MAPK/CREB pathway and local renin-angiotensin system inhibition in?… [Pubmed:29506687] |
| Neuroscience | Angiotensin II Type 1a Receptors in the Subfornical Organ Modulate Neuroinflammation in the Hypothalamic Paraventricular Nucleus in Heart Failure Rats [Pubmed:29684507] |
| American Journal of Physiology-Renal?Physiology | Role of (Pro) Renin Receptor in Albumin Overload-Induced Nephropathy in Rats [Pubmed:29846109] |
| Journal of the American Society of Hypertension | Mesenchymal stem cell–derived microvesicles alleviate pulmonary arterial hypertension by regulating renin-angiotensin system [Pubmed:29752040] |
| Evidence-based?Complementary and Alternative?Medicine? | Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects [Pubmed:29977313] |
| Brazilian Journal of Medical and Biological Research | Renovascular hypertension increases serum TNF and CX3CL1 in experimental Trypanosoma cruzi infection [Pubmed:29590257] |
| American Journal of Physiology-Heart?and Circulatory Physiology | Sex Differences in the Central and Peripheral Manifestations of Ischemia-induced Heart Failure in Rats [Pubmed: 30289294] |
| Toxicology Mechanisms and?Methods | Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats [Pubmed: 30426860] |
| Journal of?Clinical?and Aesthetic Dermatology | Detection of Angiotensin II and AT1 Receptor Concentrations in Keloid and Hypertrophic Scar. [] |
| International Journal of Ophthalmology | Serum microRNA-221 as a biomarker for diabetic retinopathy in patients associated with type 2 diabetes [Pubmed: 30588418] |
| JOURNAL OF?STRENGTH?AND CONDITIONING RESEARCH | ACE Gene Plays a Key Role in Reducing Blood Pressure in The Hyperintensive Elderly After Resistance Training [Pubmed: 30908459] |
| The?American?Journal?of?the?Medical?Sciences | T lymphocyte-based renin angiotensin system in obesity [] |
| Journal of Alzheimers Disease | Cerebrospinal Fluid Changes in the Renin-Angiotensin System in Alzheimer's Disease [Pubmed: 31594235] |
| Andrologia | Seminal levels of angiotensin II and angiotensin II type 2 receptor expression on spermatozoa in varicocele patients: Relation to fertility status [Pubmed: 31989676] |
| Molecular Medicine Reports | BPA disrupts 17?estradiol?mediated hepatic protection against ischemia/reperfusion injury in rat liver by upregulating the Ang II/AT1R signaling pathway [Pubmed: 32319667] |
| MOLECULAR MEDICINE REPORTS | The sympathetic transmitter norepinephrine inhibits VSMC proliferation induced by TGFβ by suppressing the expression of the TGFβ receptor ALK5 in aorta?… [Pubmed: 32319652] |
| Cell Mol Neurobiol | Predator Scent-Induced Sensitization of Hypertension and Anxiety-like Behaviors [Pubmed: 33201417] |
| biochemistry biophysical reports | Effect of repetitive potassium iodide on thyroid and cardiovascular functions in elderly rats [Pubmed: 33024842] |
| bioscience reports | Angiotensin II enhances the proliferation of Natural Killer/T-cell lymphoma cells via activating PI3K/Akt signaling pathway [Pubmed: 32969473] |
| AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY | A (pro) renin receptor decoy peptide PRO20 protects against adriamycin-induced nephropathy by targeting intrarenal renin-angiotensin system [Pubmed: 32865014] |
| Acta Endocrinologica-Bucharest | THE ROLE OF HIGH FOLLICULAR LEVELS OF ANGIOTENSIN II AND VASCULAR ENDOTHELIAL GROWTH FACTOR IN ANTICIPATING THE?… [Pubmed: 32685035] |
| JOURNAL OF PROTEOME RESEARCH | Proteomics and informatics for understanding phases and identifying biomarkers in COVID-19 disease [Pubmed: 32657586] |
| Scientific Reports | Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats [Pubmed: 32616735] |
| BIOMEDICINE & PHARMACOTHERAPY | Semen Brassicae reduces thoracic aortic remodeling, inflammation, and oxidative damage in spontaneously hypertensive rats [Pubmed: 32570115] |
| CLINICAL AND EXPERIMENTAL HYPERTENSION | Hyperinsulinemia rather than insulin resistance itself induces blood pressure elevation in high fat diet-fed rats [Pubmed: 32349626] |
| Evidence-Based Complementary and Alternative Medicine | Tianma Gouteng Decoction Exerts Cardiovascular Protection by Upregulating OPG and TRAIL in Spontaneously Hypertensive Rats [Pubmed: 33133215] |
| Oxidative Medicine and Cellular Longevity | Sinapic Acid Attenuates Cardiovascular Disorders in Rats by Modulating Reactive Oxygen Species and Angiotensin Receptor Expression [Pubmed: 32765804] |
| Nutrition | Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats [] |
| Dikkat Eksikli?i Hiperaktivite Bozuklu?u Tan?s? Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin D?nü?türücü Enzim (ACE), NADPH Oksidaz Enzim Serum?… [] | |
| Physiol Behav | Low-load resistance training with blood flow restriction prevent renal function decline: The role of the redox balance, angiotensin 1-7 and vasopressin [33340514] |
| ACTA PHARMACOLOGICA SINICA | The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice [33303990] |
| Int J Hypertens | The Effects of Renal Nerve Denervation on Blood Pressure and Target Organs in Different Hypertensive Rat Models [33884205] |
| Physiol Rep | (Pro) renin receptor antagonist PRO20 attenuates nephrectomy?\induced nephropathy in rats via inhibition of intrarenal RAS and Wnt/|??\catenin signaling [34057312] |
| Redox Biology | Renovascular effects of inorganic nitrate following ischemia-reperfusion of the kidney [33360353] |
| Basic and Clinical Neuroscience | Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease [33995926] |
| researchsquare | Ang-(1-7) Protects Skeletal Muscle Function in Aged Mice [] |
| Molecular Medicine Reports | Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats [34278476] |
| BMC Musculoskeletal Disorders | Ang-(1–7) protects skeletal muscle function in aged mice [34548056] |
| J Am Heart Assoc | Maternal Angiotensin II–Induced Hypertension Sensitizes Postweaning High‐Fat Diet–Elicited Hypertensive Response Through Increased Brain Reactivity in Rat?… [34482712] |
| Biomed Pharmacother | Tianma Gouteng Decoction regulates oxidative stress and inflammation in AngII-induced hypertensive mice via transcription factor EB to exert anti?… [34736077] |
| J Am Heart Assoc | Loss of the Protective Effect of Estrogen Contributes to Maternal Gestational Hypertension‐Induced Hypertensive Response Sensitization Elicited by Postweaning?… [Pubmed:35014859] |
| J Gerontol A Biol Sci Med Sci | Dysregulation of ACE-1 in normal aging and the early stages of Alzheimer's disease [Pubmed:35396835] |
| Biomedicine & Pharmacotherapy | Corynoline protects ang II-induced hypertensive heart failure by increasing PPARα and Inhibiting NF-κB pathway [Pubmed:35658238] |
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