β-內(nèi)啡肽(bEP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Beta-Endorphin (bEP)
b-EP
- 編號KSA806Hu11
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, cerebrospinal fluid and other biological fluids
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍12.35-1,000pg/mL
- 可適靈敏度4.55pg/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 6804 ¥ 11340 ¥ 20412 ¥ 39690 ¥ 68040
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特異性
試劑盒中的抗體對β-內(nèi)啡肽(bEP)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
CPA806Hu21 | β-內(nèi)啡肽(bEP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAA806Hu01 | β-內(nèi)啡肽(bEP)多克隆抗體 | WB; IHC; ICC; IP. |
LAA806Hu71 | β-內(nèi)啡肽(bEP)多克隆抗體(生物素標記) | WB; IHC; ICC. |
CEA806Hu | β-內(nèi)啡肽(bEP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CCA806Hu | β-內(nèi)啡肽(bEP)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA806Hu | β-內(nèi)啡肽(bEP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA806Hu11 | β-內(nèi)啡肽(bEP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
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Journal of Pediatric Neurosciences | Plasma and cerebrospinal fluid beta-endorphin levels show a strong association in children with cerebral malaria [] |
Chinese Journal of Physiology | Blood dopamine level enhanced by caffeine in men after treadmill running [Pubmed: 31793465] |
Free Radical Biology and Medicine | Blue light (λ= 453 nm) nitric oxide dependently induces β-endorphin production of human skin keratinocytes in-vitro and increases systemic β-endorphin levels in?… [Pubmed: 31553937] |
?Journal of Sexual Medicine | Between pleasure and pain: a pilot study on the biological mechanisms associated with BDSM interactions in dominants and submissives [Pubmed: 32044259] |
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EUROPEAN NEUROPSYCHOPHARMACOLOGY | Serum β-endorphin levels are associated with addiction to suicidal behavior: a pilot study [Pubmed: 32855024] |
J Burn Care Res | Efficacy of Acupuncture on Pain Mechanisms, Inflammatory Responses, and Wound Healing in the Acute Phase of Major Burns: An Experimental Study on Rats [34309681] |
European Journal of Integrative Medicine | Effects of transcutaneous electrical nerve stimulation applied at different frequencies during labor on hormone levels, labor pain perception, and anxiety: a randomized?… [] |
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