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β-內(nèi)啡肽(bEP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Beta-Endorphin (bEP)

b-EP

  • β-內(nèi)啡肽(bEP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • β-內(nèi)啡肽(bEP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對β-內(nèi)啡肽(bEP)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號 適用物種:Mus musculus (Mouse,小鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
CPA806Mu21 β-內(nèi)啡肽(bEP)卵白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
CEA806Mu β-內(nèi)啡肽(bEP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA806Mu β-內(nèi)啡肽(bEP)檢測試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA806Mu β-內(nèi)啡肽(bEP)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA806Mu11 β-內(nèi)啡肽(bEP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
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Brain Behav. Cerebrospinal fluid and plasma b-endorphin levels in children with cerebral malaria. [pubmed:28413714]
Journal of Cosmet Dermatol. Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties [DOI: 10.1111/jocd.12349]
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Journal of Pediatric Neurosciences Plasma and cerebrospinal fluid beta-endorphin levels show a strong association in children with cerebral malaria []
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Free Radical Biology and Medicine Blue light (λ= 453 nm) nitric oxide dependently induces β-endorphin production of human skin keratinocytes in-vitro and increases systemic β-endorphin levels in?… [Pubmed: 31553937]
?Journal of Sexual Medicine Between pleasure and pain: a pilot study on the biological mechanisms associated with BDSM interactions in dominants and submissives [Pubmed: 32044259]
JOURNAL OF AFFECTIVE DISORDERS Pain Sensitivity and Plasma Beta-Endorphin in Adolescent Non-Suicidal Self-Injury [Pubmed: 32961416]
EUROPEAN NEUROPSYCHOPHARMACOLOGY Serum β-endorphin levels are associated with addiction to suicidal behavior: a pilot study [Pubmed: 32855024]
J Burn Care Res Efficacy of Acupuncture on Pain Mechanisms, Inflammatory Responses, and Wound Healing in the Acute Phase of Major Burns: An Experimental Study on Rats [34309681]
European Journal of Integrative Medicine Effects of transcutaneous electrical nerve stimulation applied at different frequencies during labor on hormone levels, labor pain perception, and anxiety: a randomized?… []
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