膽固醇(CH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Cholesterol (CH)
- 編號KSB701Ge11
- 物種Pan-species (General,通用) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluid.
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.123-10ng/mL
- 可適靈敏度0.055ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 6854 ¥ 11424 ¥ 20563 ¥ 39984 ¥ 68544
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特異性
試劑盒中的抗體對膽固醇(CH)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Pan-species (General,通用) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
CPB701Ge11 | 膽固醇(CH)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPB701Ge21 | 膽固醇(CH)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAB701Ge01 | 膽固醇(CH)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
LAB701Ge71 | 膽固醇(CH)多克隆抗體(生物素標記) | IHC; ICC; IP. |
CEB701Ge | 膽固醇(CH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB701Ge | 膽固醇(CH)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB701Ge11 | 膽固醇(CH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Journal of?Cell?Science | Nuclear phosphatidylinositol 4, 5-bisphosphate islets contribute to efficient RNA polymerase II-dependent transcription [Pubmed:29507116] |
Journal of Diabetes Research | Regulation and Mechanism of miR-518d through the PPARα-Mediated NF-κB Pathway in the Development of Gestational Diabetes Mellitus [Pubmed: 33123597] |
Vascul Pharmacol | Immune responses against oxidized LDL as possible targets for prevention of atherosclerosis in systemic lupus erythematosus [33857652] |
Evid Based Complement Alternat Med | Effect of Acupoint Embedding on Serum Leptin and Hypothalamus Leptin Receptor Expression in Rats with Simple Obesity [34659432] |
Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313] |
Antioxidants | Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice [Pubmed:35740008] |
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