谷胱甘肽(GSH)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Glutathione (GSH)
L-Reduced Glutathione
- 編號(hào)KSA294Ge11
- 物種Pan-species (General,通用) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標(biāo)記的競(jìng)爭(zhēng)物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競(jìng)爭(zhēng)抑制酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍1.23-100ug/mL
- 可適靈敏度0.48ug/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 8597 ¥ 14328 ¥ 25790 ¥ 50148 ¥ 85968
- 欲了解實(shí)際交易價(jià)格和更多情況,請(qǐng)與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
特異性
試劑盒中的抗體對(duì)谷胱甘肽(GSH)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過(guò)夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Pan-species (General,通用) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
SPA294Ge01 | 谷胱甘肽(GSH)合成多肽 | Immunogen; Blocking Peptide. |
CPA294Ge11 | 谷胱甘肽(GSH)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA294Ge21 | 谷胱甘肽(GSH)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA294Ge41 | 谷胱甘肽(GSH)人血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAA294Ge01 | 谷胱甘肽(GSH)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
LAA294Ge71 | 谷胱甘肽(GSH)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAA294Ge22 | 谷胱甘肽(GSH)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA294Ge21 | 谷胱甘肽(GSH)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
CEA294Ge | 谷胱甘肽(GSH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA294Ge | 谷胱甘肽(GSH)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA294Ge11 | 谷胱甘肽(GSH)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
European Journal of Clinical Nutrition (2012) 66 | Evidence for augmented oxidative stress in the subjects with type 1 diabetes and their siblings: a possible preventive role for antioxidants [PubMed: 22781023] |
International Journal of Medical Sciences | Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma [PubMed: PMC3619117] |
Reproduction | Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis [Pubmed:25376627] |
Iran J Basic Med Sci | Effects of mild hypothermia therapy on the levels of glutathione in rabbit blood and cerebrospinal fluid after cardiopulmonary resuscitation [PubMed: 25810895] |
Bulletin of the Veterinary Institute in Pulawy | Oxidative Stability of Ostrich Meat Related to Duration of Linseed and Lucerne Supplementation to the Bird's Diet [View: J] |
Journal of Allergy and Clinical Immunology | Prenatal maternal distress affects atopic dermatitis in offspring mediated by oxidative stress [Pubmed:27016803] |
Oxidative Medicine and Cellular Longevity | Hydrogen sulfide protects against chronic unpredictable mild stress-induced oxidative stress in hippocampus by upreCavia (Guinea pig )lation of BDNF-TrkB pathway [journals:2153745.pdf] |
Institutional repository of Vilnius University | Rapid opioid detoxification with an implementation of the new method of gradually increasing dosage of naltrexone induction [object:elaba:15733325] |
Molecular Medicine Reports | Toxicity study of oxalicumone A, derived from a marine-derived fungus Penicillium oxalicum, in cultured renal epithelial cells [pubmed:28260084] |
Molecular?Medicine?Reports | Hepatocellular toxicity of oxalicumone A via oxidative stress injury and mitochondrial dysfunction in healthy human liver cells [pubmed:29115483] |
Cancer Medicine | Metabolomic and transcriptomic profiling of hepatocellular carcinomas in Hras12V transgenic mice [pubmed:28941178] |
Free?radical?research | Circulating cell-free DNA of methylated insulin-like growth factor-binding protein 7 predicts a poor prognosis in hepatitis B virus-associated hepatocellular carcinoma?… [Pubmed:29463155] |
Biomed?Research International | Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway [Pubmed:29770336] |
Molecular?Medicine?Reports | Epigallocatechingallate attenuates myocardial injury in a mouse model of heart failure through TGF?β1/Smad3 signaling pathway [Pubmed:29620209] |
Experimental and Therapeutic?Medicine | Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO?1 signaling pathway [Pubmed:30116359] |
Journal of Integrative Medicine | Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats [Doi: 10.1016/j.joim.2018.12.002] |
EXPERIMENTAL ANIMALS | Protective effects of sulforaphane on diabetic retinopathy: activation of the Nrf2 pathway and inhibition of NLRP3 inflammasome formation [Pubmed: 30606939] |
Acta?Biomaterialia | Porous Se@ SiO2 nanosphere-coated catheter accelerates prostatic urethra wound healing by modulating macrophage polarization through reactive oxygen species?… [Pubmed: 30753941] |
Molecular Medicine Reports | MicroRNA?21 contributes to the puerarin?induced cardioprotection via suppression of apoptosis and oxidative stress in a cell model of ischemia/reperfusion injury [Pubmed: 31115556] |
Earth and Environmental Science | Germinated-soy milk as a healthy diet to induce high antioxidant enzymes in breast milk [] |
Frontiers in Neuroscience | Hydrogen Sulfide Prevents Sleep Deprivation-Induced Hippocampal Damage by Upregulation of Sirt1 in the Hippocampus [Pubmed: 32218719] |
Oxidative Medicine and Cellular Longevity | Dexmedetomidine Attenuates Orthotopic Liver Transplantation-Induced Acute Gut Injury via α2-Adrenergic Receptor-Dependent Suppression of Oxidative Stress [Pubmed: 31827710] |
Stem Cells International | Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2?… [Pubmed: 32300366] |
Experimental and Therapeutic Medicine | Antiepileptic effects of exogenous β?hydroxybutyrate on kainic acid?induced epilepsy [Pubmed: 33101467] |
BIOLOGICAL TRACE ELEMENT RESEARCH | Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle [Pubmed: 32583225] |
Toxicology | Formaldehyde induces ferroptosis in hippocampal neuronal cells by upregulation of the Warburg effect [33259821] |
CELLULAR AND MOLECULAR LIFE SCIENCES | Glutamine deficiency promotes stemness and chemoresistance in tumor cells through DRP1-induced mitochondrial fragmentation [33895866] |
Exp Ther Med | Naringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic rats [34093766] |
Experimental and Therapeutic Medicine | MicroRNA-145-5p aggravates cell apoptosis and oxidative stress in tongue squamous cell carcinoma [33732346] |
chemosphere | Oxidative stress-and mitochondrial dysfunction-mediated cytotoxicity by silica nanoparticle in lung epithelial cells from metabolomic perspective [33662726] |
Molecular Medicine Reports | Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats [34278476] |
J Healthc Eng | κ-Opioid Receptor Agonist Ameliorates Postoperative Neurocognitive Disorder by Activating the Ca2+/CaMKII/CREB Pathway [34608407] |
Cell Death Discov | Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl rats [Pubmed:35022384] |
J Dairy Sci | Intake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver disease [Pubmed:35086715] |
Antioxidants | Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice [Pubmed:35740008] |
J Inflamm Res | The κ-Opioid Receptor Agonist U50488H Ameliorates Neuropathic Pain Through the Ca2+/CaMKII/CREB Pathway in Rats [Pubmed:35645576] |
留言咨詢