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組胺(HA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Histamine (HA)

Histamin

  • 組胺(HA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 組胺(HA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對Histamine (HA)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號 適用物種:Pan-species (General,通用) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
CPA927Ge41 組胺(HA)人血清白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
CPA927Ge11 組胺(HA)牛血清白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
CPA927Ge21 組胺(HA)卵白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
PAA927Ge01 組胺(HA)多克隆抗體 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA927Ge71 組胺(HA)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
LAA927Ge81 組胺(HA)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
MAA927Ge21 組胺(HA)單克隆抗體 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
CEA927Ge 組胺(HA)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA927Ge 組胺(HA)檢測試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA927Ge 組胺(HA)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA927Ge11 組胺(HA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It(ELISA Kit) Yourself"

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Allergy & Clinical Immunology PEST-domain-enriched tyrosine phosphatase and glucocorticoids as regulators of anaphylaxis in mice [Pubmed: 21981059]
Asian Pacific Journal of Allergy and Immunology Comparison of sensitization between β-lactoglobulin and its hydrolysates [Digital: 875]
J Chromatogr A Integrative analysis of proteomics and metabolomics of anaphylactoid reaction induced by Xuesaitong injection [PubMed: 26372445]
Neurogastroenterology & Motility Expression of inducible nitric oxide synthase in mast cells contributes to the reCavia (Guinea pig )lation of inflammatory cytokines in irritable bowel syndrome with diarrhea [Pubmed:26940641]
Italian Journal of Animal Science Induction of immune responses and allergic reactions in piglets by injecting glycinin [doi:1828051X.2016.1144488]
Int J Clin Exp Pathol Effects of Dectin-1 on the mast cells in allergic conjunctivitis and its underlying mechanism [files:ijcep0019305.pdf]
PLoS One. Proteomics Study on Nonallergic Hypersensitivity Induced by Compound 4880 and Ovalbumin [pmc:PMC4734762]
Int Immunopharmacol. Evaluation of anaphylactoid constituents in vitro and in vivo. [pubmed:27984711]
Journal of Cosmet Dermatol. Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties [DOI: 10.1111/jocd.12349]
INTERNATIONAL JOURNAL OF MOLECULAR?SCIENCES? Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model [Pubmed: 30781605]
Royal?Society?Open?Science Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats []
International?Journal?of?Pharmaceutical?Sciences?and?Research MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL []
Allergy Artemisia annua sublingual immunotherapy for seasonal allergic rhinitis: a randomized controlled trial [Pubmed: 32030780]
MOLECULAR IMMUNOLOGY Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection [Pubmed: 32302806]
bioscience reports Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767]
Nature Communications A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapy [Pubmed: 32332752]
CCR3?shRNA promotes apoptosis and inhibits chemotaxis and degranulation of mouse mast cells [Pubmed: 32742345]
ANALYTICA CHIMICA ACTA Multiplexed fluorescence immunoassay combined with magnetic separation using upconversion nanoparticles as multicolor labels for the simultaneous detection of?… [Pubmed: 32892931]
Annals of Tropical Medicine & Public Health Study of some hematological and immunological parameters associated with the infection of intestinal parasites in the holy city of Kerbala, Iraq []
pharmacol res& perspect Pharmacokinetics and safety of TCMCB07, a melanocortin-4 antagonist peptide in dogs [34014033]
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