羥脯氨酸(Hyp)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Hydroxyproline (Hyp)
(2S,4R)-4-Hydroxyproline; L-Hydroxyproline(C5H9O3N)
- 編號KSA621Ge11
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標(biāo)記的競爭物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍61.7-5000ng/mL
- 可適靈敏度24.1ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 8597 ¥ 14328 ¥ 25790 ¥ 50148 ¥ 85968
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對羥脯氨酸(Hyp)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CPA621Ge11 | 羥脯氨酸(Hyp)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA621Ge21 | 羥脯氨酸(Hyp)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA621Ge01 | 羥脯氨酸(Hyp)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEA621Ge | 羥脯氨酸(Hyp)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA621Ge | 羥脯氨酸(Hyp)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA621Ge | 羥脯氨酸(Hyp)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA621Ge11 | 羥脯氨酸(Hyp)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLoS One. | Spleen-Derived Interleukin-10 Downregulates the Severity of High-Fat Diet-Induced Non-Alcoholic Fatty Pancreas Disease [PubMed: PMC3532347] |
| Indian Journal of Virology | Prediction of fibrosis in hepatitis C patients: assessment using hydroxyproline and oxidative stress biomarkers [Springer: Source] |
| Journal of Functional Foods | Royal jelly affects collagen crosslinking in bone of ovariectomized rats [Sciencedirect:S1756464614000346] |
| J Surg Res | Tanshinone IIA ameliorates bleomycin-induced pulmonary fibrosis and inhibits transforming growth factor-beta-β–dependent epithelial to mesenchymal transition [PubMed: 25911951] |
| Life Sciences | Theophylline, an old drug with multi-faceted effects: Its potential benefits in immunological liver injury in rats [PubMed: 26165752] |
| Original Investigation | Effects of 4% Icodextrin on Experimental Spinal Epidural Fibrosis [pdf:JTNEPUB_15079_online.pdf] |
| Life?Sciences | Celecoxib?attenuates?depressive-like?behavior?associated?with?immunological?liver?injury?inC57BL/6?mice?through TNF-α and NF-κb?dependent?mechanisms. [pubmed:27580523] |
| Annals of Surgical Treatment and Research | Sustained-released mixture of vascular endothelial growth factor 165 and fibrin glue strengthens healing of ileal anastomoses in a rabbit model with … [doi.org:10.4174] |
| Journal of?Cellular?Biochemistry | Effects of panax notoginseng saponin on the pathological ultrastructure and serum IL‐6 and IL‐8 in pulmonary fibrosis in rabbits [Pubmed:29932250] |
| Canadian Journal of Physiology and Pharmacology | Renin–angiotensin system inhibition ameliorates CCl-induced liver fibrosis in mice through the inactivation of nuclear transcription factor kappa B [10.1139:cjpp-2017-0728] |
| Experimental and Therapeutic Medicine | Pirfenidone suppresses bleomycin?induced pulmonary fibrosis and periostin expression in rats [10.3892:etm.2018.6378] |
| Cell Death & Disease | Therapeutic effect of hepatocyte growth factor-overexpressing bone marrow-derived mesenchymal stem cells on CCl 4-induced hepatocirrhosis. [] |
| International wound journal | The effects of platelet‐rich‐plasma gel application to the colonic anastomosis in hyperthermic intraperitoneal chemotherapy: An experimental rat model [Pubmed: 31448554] |
| ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Pantoea agglomerans chronic exposure induces epithelial-mesenchymal transition in human lung epithelial cells and mice lungs [Pubmed: 32146192] |
| Annals of Surgical Treatment and Research | Is platelet-rich plasma improves the anastomotic healing in hyperthermic intraperitoneal chemotherapy with oxaliplatin: an experimental rat study [Pubmed: 32051817] |
| Arch Med Res | Protective Effects of Contractubex? on Stricture Formation after Experimental Corrosive Esophageal Burns in Rats [Pubmed: 32654881] |
| International Medical Journal? | Effect of Oral Channa striatus in Laparotomy Wound Healing of Malnourished Rats. [] |
| Dis Marker | Handgrip Strength and Vitamin D as Predictors of Liver Fibrosis and Malnutrition in Chronic Hepatitis C Patients [33884041] |
| PLoS One | Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies [33999928] |
| Front Cell Dev Biol | Curdione and Schisandrin C Synergistically Reverse Hepatic Fibrosis via Modulating the TGF-β Pathway and Inhibiting Oxidative Stress [34858986] |
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