免疫球蛋白A(IgA)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Immunoglobulin A (IgA)
- 編號(hào)KSA546Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍1.56-100ng/mL
- 可適靈敏度0.69ng/mL
- 應(yīng)用自己動(dòng)手制作ELISA試劑盒的主要材料
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 6048 ¥ 10080 ¥ 18144 ¥ 35280 ¥ 60480
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特異性
試劑盒中的抗體對(duì)免疫球蛋白A(IgA)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過(guò)夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開(kāi)封,在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
NPA546Hu01 | 免疫球蛋白A(IgA)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA546Hu05 | 免疫球蛋白A(IgA)多克隆抗體 | WB; IHC; ICC; IP. |
PAA546Hu01 | 免疫球蛋白A(IgA)多克隆抗體 | WB; IHC; ICC; IP. |
SAA546Hu09 | 兔抗人IgA多克隆抗體(辣根過(guò)氧化物酶標(biāo)記) | ELISA; WB; IHC; ICC. |
LAA546Hu71 | 免疫球蛋白A(IgA)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
SEA546Hu | 免疫球蛋白A(IgA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA546Hu | 免疫球蛋白A(IgA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
AEA546Hu | 抗免疫球蛋白A抗體(Anti-IgA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
SCA546Hu | 免疫球蛋白A(IgA)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA546Hu | 免疫球蛋白A(IgA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA546Hu01 | 免疫球蛋白A(IgA)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
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Poultry Science | Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88 [Pubmed: 24570422 ] |
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Food & Function | Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice [Pubmed:25308407] |
Pediatrics | Oropharyngeal colostrum administration in extremely premature infants: an RCT. [Pubmed:25624376] |
Front Microbiol | Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis [PubMed: 26217323] |
Food and Agricultural Immunology | Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers [10.108:09540105.2017.1311308 ] |
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SAGE?Open?Medicine | Altered immunoglobulins (A and G) in Ghanaian patients with type 2 diabetes [Pubmed:29623201] |
Nephrology Dialysis Transplantation | The gut flora modulates intestinal barrier integrity but not progression of chronic kidney disease in hyperoxaluria-related nephrocalcinosis [Pubmed: 31081025] |
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International Journal of Biological Macromolecules | Effects of polysaccharides from Yingshan Yunwu tea on meat quality, immune status and intestinal microflora in chickens [Pubmed: 32224178] |
Effect Long-Term Exposure to Different Doses of Lipopolysaccharides on the Intestinal Mucosal Immune Barrier [] | |
Italian Journal of Animal Science | Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the?… [] |
博士論文 | Women Scientist Scientist Mentor [] |
Acta Paediatr | MicroRNA profiling in Chinese children with Henoch?\Schonlein purpura and association between selected microRNAs and inflammatory biomarkers [33533510] |
Environ Pollut | Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure [34090073] |
J Ethnopharmacol | Monascus ruber fermented Panax ginseng ameliorates lipid metabolism disorders and modulate gut microbiota in rats fed a high-fat diet [34098018] |
Microbiology Spectrum | Dietary Supplementation of Inulin Ameliorates Subclinical Mastitis via Regulation of Rumen Microbial Community and Metabolites in Dairy Cows [34494854] |
Food Research International | Sulfated modification enhances the immunomodulatory effect of Cyclocarya paliurus polysaccharide on cyclophosphamide-induced immunosuppressed mice through?… [34865774] |
Food Funct | Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein Components [Pubmed:35438093] |
Food and Agricultural Immunology | The effect of the multi-strain probiotic preparation EM Bokashi? on selected parameters of the cellular immune response in pigs [] |
Journal of Functional Foods | Bovine lactoferricin ameliorates intestinal inflammation and mucosal barrier lesions in colitis through NF-κB/NLRP3 signaling pathways [] |
Journal of Dentistr Journal of Dentistry Indonesia | Salivary IgA Depression in Drug-Influenced Gingival Enlargement among Hypertensive Patients [] |
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