降鈣素原(PCT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Procalcitonin (PCT)
Pro-Calcitonin
- 編號KSA689Ga01
- 物種Chicken (Gallus，雞) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍31.2-2000pg/mL
- 可適靈敏度12.7pg/mL
- 應(yīng)用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 7434 ¥ 12390 ¥ 22302 ¥ 43365 ¥ 74340
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對降鈣素原(PCT)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Chicken (Gallus，雞) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA689Ga01 | 降鈣素原(PCT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA689Ga01 | 降鈣素原(PCT)多克隆抗體 | WB; IHC; ICC; IP. |
| KSA689Ga01 | 降鈣素原(PCT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Scandinavian Journal of Infectious Diseases | Evaluation of neutrophilic CD64, interleukin 10 and procalcitonin as diagnostic markers of early- and late-onset neonatal sepsis [PubMed: 20085423] |
| International Journal of Radiation Oncology ? Biology ? Physics | Effects of Berberine Against Radiation-Induced Intestinal Injury in Mice [PubMed: 20637981] |
| Ulus Travma Acil Cerrahi Derg | Diagnostic and prognostic value of procalcitonin and phosphorus in acute mesenteric ischemia [PubMed: 21935794] |
| Egyptian Journal of Bronchology | Serum procalcitonin and its relation to the outcome of ventilator-associated pneumoniain children [Essbronchology: Source] |
| University of Waikato | The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis [Waikato:Source] |
| Egyptian Journal of Medical Microbiology | Evaluation of the Diagnostic Utility of Fc Gamma Receptor Type 1 (nCD64) and Procalcitonin in Comparison with Conventional Methods for Diagnosis of Early Onset Neonatal Sepsis [Ejmm:Source] |
| Nigerian Medical Journal | Efficacy of measuring procalcitonin levels in determination of prognosis and early diagnosis of bacterial resistance in sepsis [Pubmed:25657488] |
| Wiley online library | The use and value of procalcitonin in solid organ transplantation [PubMed: 25996831] |
| Balkan Med J | Diagnostic Value of Procalcitonin Levels in Acute Mesenteric Ischemia [PubMed: 26185718] |
| The Journal of Clinical Endocrinology & Metabolism | Macrocalcitonin is a novel pitfall in the routine of serum calcitonin immunoassay [PubMed: 26647152] |
| Journal of Human Hypertension | Procalcitonin and Pentraxin-3: Current biomarkers in inflammation in white coat hypertension [PubMed: 26040439] |
| Medical Science Monitor | Variation?of?Circulating?Inflammatory?Mediators?in?Staphylococcus?aureus?and?Escherichia?coli?BloodstreamInfection. [Pubmed:26772168] |
| International Immunopharmacology | Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery [Pubmed:26802602] |
| Pancreatology. | Early prediction of intestinal mucosal barrier function impairment by elevated serum procalcitonin in rats with severe acute pancreatitis. [Pubmed:26804005] |
| Acta?Medica | Risk Factors?of?Acute?Pancreatitis?in?Oral?Double Balloon Enteroscopy. [pubmed:27638962] |
| The Clinical Respiratory Journal | Changes in inflammatory mediators as a result of intermittent hypoxia in obstructive sleep apnea syndrome [pubmed:28960823] |
| SHOCK | INTERLEUKIN-1 RECEPTOR 2: A NEW BIOMARKER FOR SEPSIS DIAGNOSIS AND GRAM-NEGATIVE/GRAM-POSITIVE BACTERIAL DIFFERENTIATION [pubmed:27984536] |
| Cellular?Physiology and Biochemistry | Hepatic Macrophages are the Cell Source of Hepatic Procalcitonin in Acute Liver Failure [Pubmed:29913443] |
| Archives of Medical Science | Inflammatory mediators in the diagnosis and treatment of acute pancreatitis: pentraxin-3, procalcitonin and myeloperoxidase [Pubmed:29593801] |
| Journal of Thoracic Disease | Expression pattern of soluble triggering receptor expressed on myeloid cells-1 in mice with Acinetobacter baumannii colonization and infection in the lung [Pubmed:29707313] |
| Asian?Journal of Psychiatry | Procalcitonin and C-reactive protein as peripheral inflammatory markers in antipsychotic drug-free schizophrenia patients [Pubmed:29723719] |
| European Review for Medical and Pharmacological Sciences | The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia. [Pubmed: 31298341] |
| Medicina | Modestly Elevated Serum Procalcitonin Levels in Patients with Rheumatoid Arthritis Free of Active Infection [Pubmed: 33080909] |
| MEDIATORS OF INFLAMMATION | Reevaluation of Lung Injury in TNF-Induced Shock: The Role of the Acid Sphingomyelinase [Pubmed: 32410851] |
| Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes [] | |
| Journal of Immunology Research | Moesin Is a Novel Biomarker of Endothelial Injury in Sepsis [33628853] |
| Journal of Medical Biochemistry | Circulating nuclear factor-kappa B mediates cancer-associated inflammation in human breast and colon cancer [33776564] |
| Surg Endosc | Original contribution: sleeve gastrectomy reduces soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) levels in patients with morbid obesity [Pubmed:35044516] |
| Bone Res | Procalcitonin is expressed in osteoblasts and limits bone resorption through inhibition of macrophage migration during intermittent PTH treatment [Pubmed:35087025] |
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