Ⅲ型前膠原氨基端原肽(PIIINP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Procollagen III N-Terminal Propeptide (PIIINP)
P3NP; N-Propeptide Of Type III Procollagen; Procollagen III Amino Terminal Propeptide
- 編號KSA573Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍3.12-200ng/mL
- 可適靈敏度1.37ng/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7344 ¥ 12240 ¥ 22032 ¥ 42840 ¥ 73440
- 欲了解實際交易價格和更多情況,請與當?shù)亟?jīng)銷商聯(lián)系!
特異性
試劑盒中的抗體對Ⅲ型前膠原氨基端原肽(PIIINP)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
RPA573Hu02 | Ⅲ型前膠原氨基端原肽(PIIINP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA573Hu62 | Ⅲ型前膠原氨基端原肽(PIIINP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA573Hu63 | Ⅲ型前膠原氨基端原肽(PIIINP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA573Hu01 | Ⅲ型前膠原氨基端原肽(PIIINP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA573Hu61 | Ⅲ型前膠原氨基端原肽(PIIINP)活性蛋白 | Cell?culture;?Activity?Assays. |
EPA573Hu61 | Ⅲ型前膠原氨基端原肽(PIIINP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA573Hu21 | Ⅲ型前膠原氨基端原肽(PIIINP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
SPA573Hu02 | Ⅲ型前膠原氨基端原肽(PIIINP)合成多肽 | Immunogen; Blocking Peptide. |
CPA573Hu22 | Ⅲ型前膠原氨基端原肽(PIIINP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA573Hu31 | Ⅲ型前膠原氨基端原肽(PIIINP)鑰孔血藍蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
SPA573Hu01 | Ⅲ型前膠原氨基端原肽(PIIINP)合成多肽 | Immunogen; Blocking Peptide. |
SPA573Hu71 | Ⅲ型前膠原氨基端原肽(PIIINP)合成多肽 | Immunogen; Blocking Peptide. |
PAA573Hu01 | Ⅲ型前膠原氨基端原肽(PIIINP)多克隆抗體 | WB; IHC; ICC; IP. |
PAA573Hu02 | Ⅲ型前膠原氨基端原肽(PIIINP)多克隆抗體 | WB; IHC; ICC; IP. |
PAA573Hu09 | Ⅲ型前膠原氨基端原肽(PIIINP)多克隆抗體 | WB; IHC; ICC; IP. |
PAA573Hu08 | Ⅲ型前膠原氨基端原肽(PIIINP)多克隆抗體 | WB; IHC; ICC; IP. |
LAA573Hu71 | Ⅲ型前膠原氨基端原肽(PIIINP)多克隆抗體(生物素標記) | WB; IHC; ICC. |
MAA573Hu23 | Ⅲ型前膠原氨基端原肽(PIIINP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA573Hu24 | Ⅲ型前膠原氨基端原肽(PIIINP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA573Hu27 | Ⅲ型前膠原氨基端原肽(PIIINP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA573Hu28 | Ⅲ型前膠原氨基端原肽(PIIINP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA573Hu26 | Ⅲ型前膠原氨基端原肽(PIIINP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA573Hu29 | Ⅲ型前膠原氨基端原肽(PIIINP)單克隆抗體 | WB |
MAA573Hu25 | Ⅲ型前膠原氨基端原肽(PIIINP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA573Hu21 | Ⅲ型前膠原氨基端原肽(PIIINP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA573Hu22 | Ⅲ型前膠原氨基端原肽(PIIINP)單克隆抗體 | WB; IHC; ICC; IP. |
FAA573Hu04 | 抗Ⅲ型前膠原氨基端原肽(PIIINP)單克隆抗體 | Flow cytometry. |
FAA573Hu08 | 抗Ⅲ型前膠原氨基端原肽(PIIINP)單克隆抗體 | Flow cytometry. |
FAA573Hu06 | 抗Ⅲ型前膠原氨基端原肽(PIIINP)單克隆抗體 | Flow cytometry. |
FAA573Hu02 | 抗Ⅲ型前膠原氨基端原肽(PIIINP)單克隆抗體 | Flow cytometry. |
LAA573Hu94 | Ⅲ型前膠原氨基端原肽(PIIINP)單克隆抗體(辣根過氧化物酶標記) | IHC; ICC |
HEA573Hu | Ⅲ型前膠原氨基端原肽(PIIINP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEA573Hu | Ⅲ型前膠原氨基端原肽(PIIINP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEA573Hu | Ⅲ型前膠原氨基端原肽(PIIINP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA573Hu | Ⅲ型前膠原氨基端原肽(PIIINP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
PSA573Hu01 | Ⅲ型前膠原氨基端原肽(PIIINP)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA573Hu11 | Ⅲ型前膠原氨基端原肽(PIIINP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
KSA573Hu01 | Ⅲ型前膠原氨基端原肽(PIIINP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
雜志 | 參考文獻 |
Molecular Biology Reports | Characterization of the specific and sustained GH1 expression induced by rAAV2/1 in normal adult male rats [PubMed: 20204528] |
Obesity Surgery | A Biomarker Panel for Non-alcoholic Steatohepatitis (NASH) and NASH-Related Fibrosis [PubMed: 20532833] |
Liver Biopsy | Comparison of Five Liver Fibrosis Indexes with Serum Levels of Laminin and N Terminal Peptide of Procollagen Type III in Chronic Hepatitis Patients [Intechopen: 18785] |
Trials | Is there benefit in optimising heart failure treatment in over-80 year-old patients? (HF-80 study): study protocol for a randomized controlled trial. [Trials: 1745-6215] |
American Journal of Hypertension | Aliskiren and Perindopril Reduce the Levels of Transforming Growth Factor-β in Patients With Non-Diabetic Kidney Disease [Nature: 201214] |
Arthritis Research and Therapy | Raised circulating tenascin-C in rheumatoid arthritis. [PubMed: 23193984] |
American Heart Journal | Serum markers of deranged myocardial collagen turnover: their relation to malignant ventricular arrhythmias in cardioverter-defibrillator recipients with heart failure. [PubMed: 23067911] |
hepatology research | Diagnostic potential of serum direct markers and non-invasive fibrosis models in patients with chronic hepatitis B. [PubMed: 22734888] |
Digestive and Liver Disease | Effects of coffee consumption in chronic hepatitis C: a randomized controlled trial [Pubmed: 23238034] |
Journal of Infectious Diseases | Procollagen III N-terminal propeptide and desmosine are released by matrix destruction in pulmonary tuberculosis [Pubmed: 23922364] |
International Urogynecology Journal | Prevalence, etiology and risk factors of pelvic organ prolapse in premenopausal primiparous women [Springer: Source] |
Journal of Clinical and Experimental Hepatology | Protective Effects of Norursodeoxycholic Acid Versus Ursodeoxycholic Acid on Thioacetamide-induced Rat Liver Fibrosis [ScienceDirect: S0973688314000073] |
Der Urologe | Retroperitoneale Fibrose [Springer:Source] |
Ultrasound in Obstetrics & Gynecology | The status of the pelvic floor in young primiparous women [Wiley:Source] |
PLoS One. | Clinical significance of markers of collagen metabolism in rheumatic mitral valve disease [Pubmed:24603967] |
Clin Chim Acta. | Serum and urinary procollagen III aminoterminal propeptide as a biomarker of obstructive nephropathy in children [Pubmed:24768785] |
BMC Gastroenterol. | Fast food diet with CCl4 micro-dose induced hepatic-fibrosis-a novel animal model [Pubmed:24884574] |
Toxicology. | DNMT3A silencing RASSF1A promotes cardiac fibrosis through upregulation of ERK1/2 [Pubmed:24945829] |
Southeast Asian J Trop Med Public Health. | Correlation of HBsAg titers with serum fibrotic maker in patients with chronic hepatitis B infection. [Pubmed:24974647] |
The American Journal of Cardiology | Relation of burden of myocardial fibrosis to malignant ventricular arrhythmias and outcomes in Fabry disease [Pubmed:25073565] |
Ann Rheum Dis. | B-cell depletion attenuates serological biomarkers of fibrosis and myofibroblast activation in IgG4-related disease [Pubmed:25143523] |
Scientific Reports | Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with Hypertension [PubMed: 25747153] |
Int J Mol Sci | The Multi-Biomarker Approach for Heart Failure in Patients with Hypertension [PubMed: 25984599] |
Viral Hepatitis Journal | The Relationship of the Degree of Hepatic Fibrosis with Hyaluronic Acid, Type 4 Collagen, and Procollagen Type 3 N-Terminal Peptide Levels in Patients with Chronic Viral Hepatitis [Openview: 8A0B8C97D72C61741371F33508760B75] |
International Journal of Experimental Pathology | Pathological characterization and morphometric analysis of hepatic lesions in SHRSP5/Dmcr, an experimental non-alcoholic steatohepatitis model, induced by high-fat and high-cholesterol diet [doi:10.1111] |
Heart Rhythm.? | Prognostic value of collagen turnover biomarkers in cardiac resynchronization therapy: A subanalysis of the TRUST CRT randomized trial population [Pubmed:26776557] |
BMJ Open Sport Exerc Med | Procollagen markers in microdialysate can predict patient outcome after Achilles tendon rupture [content:2] |
Inflammation Research | Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy [pubmed:27516211] |
Heart & Vessels | Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [pubmed:28004175] |
american journal of physiology-renal physiology | Extracellular microRNA signature in chronic kidney disease. [pubmed:28077372] |
Cellular?Physiology and Biochemistry | Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy [pubmed:27889753] |
PLoS One. | Procollagen?C-Proteinase?Enhancer?1?(PCPE-1) as a?Plasma?Marker?of?Muscleand?Liver Fibrosis?in?Mice. [pubmed:27458976] |
International?Journal?of?Cardiology | 5-methoxytryptophan?is a?potential?marker?for?post-myocardial?infarction?heart?failure - apreliminary?approach?to?clinical?utility. [pubmed:27526355] |
Transplant Proc | Urinary Type III Procollagen Is Associated With Chronic Allograft Dysfunction and Predicts Graft Survival. [pubmed:28219585] |
Knee Surg Sports Traumatol Arthrosc | Achilles tendon rupture healing is enhanced by intermittent pneumatic compression upregulating collagen type I synthesis [pubmed:28668970] |
Antiviral Therapy | Association of HIV infection with biomarkers of kidney injury and fibrosis in the Multicenter AIDS Cohort Study [pubmed:28054933] |
Molecular?Medicine?Reports | Profilin?1 contributes to cardiac injury induced by advanced glycation end?products in rats [pubmed:28901418] |
Biomarkers Medicial | Relationship of biomarkers of extracellular matrix with myocardial function in Type 2 diabetes mellitus [pubmed:28685602] |
Heart and Vessels | Left ventricular reverse remodeling is not related to biopsy?detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [10.1007/s00380-016-0930-y] |
Clinical Infectious Diseases | Matrix Degradation in Human Immunodeficiency Virus Type 1–Associated Tuberculosis and Tuberculosis Immune Reconstitution Inflammatory Syndrome: A Prospective Observational Study [10.1093/cid/cix231] |
Cytokine | 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis. [pubmed:28460256] |
Advanced Healthcare Materials | Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events. [pubmed:29120858] |
American Journal of?Kidney?Diseases | Urinary Biomarkers of Kidney Tubular Damage and Risk of Cardiovascular Disease and Mortality in Elders [Pubmed:29602632] |
Angiology | The Association of Biomarkers of Inflammation and Extracellular Matrix Degradation With the Risk of Abdominal Aortic Aneurysm: The ARIC Study [article:10.1007] |
Hernia | Circulating matrix metalloproteinases and procollagen propeptides in inguinal hernia [Pubmed:29484522] |
Cardiovascular Journal of Africa | Assessment of myocardial fibrosis by late gadolinium enhancement imaging and biomarkers of collagen metabolism in chronic rheumatic mitral regurgitation. [Pubmed:29443354] |
Turk Kardiyol Dern Ars.? | Association between reverse electrical remodeling and cardiac fibrosis markers in patients with cardiac resynchronization therapy [Pubmed:29512624] |
Кардиоваскулярная терапия и профилактика | N-терминальный пропептид проколлагена III типа в качестве возможного сывороточного маркера фиброза миокарда у больных сахарным диабетом 2?… [:] |
Clinical?Infectious?Diseases | Improvement in Hepatic Fibrosis Biomarkers Associated with Chemokine Receptor Inactivation through Mutation or Therapeutic Blockade [Pubmed: 30239650] |
Anatolian Journal of Cardiology | microRNA-29a inhibits cardiac fibrosis in Sprague-Dawley rats by downregulating the expression of DNMT3A. [Pubmed: 30297596] |
Contemporary?Clinical?Trials | Methods to improve the noninvasive diagnosis and assessment of disease severity in children with suspected nonalcoholic fatty liver disease (NAFLD): Study design [Doi: 10.1016/j.cct.2018.10.012] |
Arthritis?Research &?Therapy | Association of serum levels of fibrosis-related biomarkers with disease activity in patients with IgG4-related disease [Pubmed: 30547825] |
American Journal of Respiratory and?Critical?Care?Medicine | Identification and Modulation of Microenvironment is Crucial for Effective MSC Therapy in Acute Lung Injury [Pubmed: 30521764] |
Direktor: Prof. Dr. med. Andreas Mügge [] | |
Scientific Reports | Low fibrosis biomarker levels predict cardiac resynchronization therapy response [Pubmed: 30988339] |
American Journal of Translational Research | Development and validation of an in vitro 3D model of NASH with severe fibrotic phenotype [Pubmed: 30972180] |
Food and Chemical Toxicology | Gum Acacia mitigates diclofenac nephrotoxicity by targeting monocyte chemoattractant protein-1, complement receptor-1 and pro-apoptotic pathways [Pubmed: 31042592] |
Digestive diseases and sciences | Validation and Refinement of Noninvasive Methods to Assess Hepatic Fibrosis: Magnetic Resonance Elastography Versus Enhanced Liver Fibrosis Index [Pubmed: 31468264] |
Journal of Diabetes Research | Type 2 Diabetes Mellitus and Chronic Heart Failure with Midrange and Preserved Ejection Fraction: A Focus on Serum Biomarkers of Fibrosis [Pubmed: 33224989] |
BMC GASTROENTEROLOGY | Changes in hepatic fibrosis and vitamin D levels after viral hepatitis C eradication using direct-acting antiviral therapy [Pubmed: 33069226] |
european heart journal | A randomized controlled trial of dapagliflozin on left ventricular hypertrophy in people with type two diabetes: the DAPA-LVH trial [Pubmed: 32578850] |
Ассоциации толщины эпикардиального жира и циркулирующих маркеров фиброза миокарда у пациентов с инфарктом миокарда [] | |
J Am Geriatr Soc | Association of Non?\Steroidal Anti?\Inflammatory Drugs with Kidney Health in Ambulatory Older Adults [33305369] |
Aging (Albany NY) | Prognostic potential of cardiac structural and functional parameters and N-terminal propeptide of type III procollagen in predicting cardiac fibrosis one year after??- [33431713] |
PeerJ | Effect of vitamin D supplementation in patients with chronic hepatitis C after direct-acting antiviral treatment: a randomized, double-blind, placebo-controlled trial [33614272] |
Open Forum Infectious Diseases | Soluble CD163 Identifies Those at Risk for Increased Hepatic Inflammation & Fibrosis [34104667] |
European Heart Journal | A randomized controlled trial of dapagliflozin on left ventricular hypertrophy in people with type two diabetes [32578850] |
Nutrients | The Effect of Daily Methylsulfonylmethane (MSM) Consumption on High-Density Lipoprotein Cholesterol in Healthy Overweight and Obese Adults: A Randomized?… [34684621] |
J Diabetes Res | Association between Markers of Fibrosis and Heart Failure Incidence in Patients with Type 2 Diabetes Mellitus [34778465] |
Scandinavian Cardiovascular Journal | Dual integrin αvβ3 and αvβ5 blockade attenuates cardiac dysfunction by reducing fibrosis in a rat model of doxorubicin-induced cardiomyopathy [34296634] |
J. Clin. Med | Simple Predictors for Cardiac Fibrosis in Patients with Type 2 Diabetes Mellitus: The Role of Circulating Biomarkers and Pulse Wave Velocity [Pubmed:35628969] |
Biomolecules | Increase in Serum MMP-9 and TIMP-1 Concentrations during Alcohol Intoxication in Adolescents—A Preliminary Study [Pubmed:35625637] |
留言咨詢