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胰蛋白酶原激活肽(TAP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Trypsinogen Activation Peptide (TAP)

  • 胰蛋白酶原激活肽(TAP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 胰蛋白酶原激活肽(TAP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)胰蛋白酶原激活肽(TAP)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào) 適用物種:Rattus norvegicus (Rat,大鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
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LMA634Ra 胰蛋白酶原激活肽(TAP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA634Ra11 胰蛋白酶原激活肽(TAP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Diabetologia Diabetes aggravates acute pancreatitis and inhibits pancreas regeneration in mice [SpringerLink: x4l01435t34452jr]
Molecular Biology Reports Prior peritoneal lavage with hot 0.9% saline induces HSP70 expression and protects against cerulein-induced acute pancreatitis in rats [Pubmed: 23096089]
Journal of the Pancreas Case report of mannose-binding lectin (MBL) deficiency and postoperative sepsis and coagulopathy in a patient following total pancreatectomy for chronic pancreatitis. [Pubmed:25262717]
Journal of Gastroenterology A small molecule inhibitor of NFκB blocks ER stress and the NLRP3 inflammasome and prevents progression of pancreatitis [pubmed:27418337]
Indian Journal of Pharmaceutical Sciences Effects of Calcium Channel Blockers on Trypsinogen Activation and Severity of Cerulein-induced Acute Pancreatitis in Rats [IJPS:Source]
Tropical Journal of Pharmaceutical Research Investigation of non-pharmaceutical cures for acute pancreatitis induced by cerulein in rats []
Pancreas The Regulatory Effect of the Kinase Inhibitor PD98059 on Autophagic Flux During Trypsinogen Activation in Pancreatic Acinar Cells [Pubmed: 32011537]
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