血管內(nèi)皮生長(zhǎng)因子121(VEGF121)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Vascular Endothelial Growth Factor 121 (VEGF121)
- 編號(hào)KSB851Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍15.6-1000pg/mL
- 可適靈敏度6.5pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 4806 ¥ 8010 ¥ 14418 ¥ 28035 ¥ 48060
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特異性
試劑盒中的抗體對(duì)血管內(nèi)皮生長(zhǎng)因子121(VEGF121)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過(guò)夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開(kāi)封,在4度保存時(shí)間為1個(gè)月。
配套試劑
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相關(guān)產(chǎn)品
編號(hào) | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB851Hu01 | 血管內(nèi)皮生長(zhǎng)因子121(VEGF121)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB851Hu02 | 血管內(nèi)皮生長(zhǎng)因子121(VEGF121)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB851Hu01 | 血管內(nèi)皮生長(zhǎng)因子121(VEGF121)活性蛋白 | Cell?culture;?Activity?Assays. |
PAB851Hu01 | 血管內(nèi)皮生長(zhǎng)因子121(VEGF121)多克隆抗體 | WB; IHC; ICC; IP. |
PAB851Hu02 | 血管內(nèi)皮生長(zhǎng)因子121(VEGF121)多克隆抗體 | WB; IHC; ICC; IP. |
LAB851Hu71 | 血管內(nèi)皮生長(zhǎng)因子121(VEGF121)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAB851Hu22 | 血管內(nèi)皮生長(zhǎng)因子121(VEGF121)單克隆抗體 | WB; IHC; ICC; IP. |
MAB851Hu23 | 血管內(nèi)皮生長(zhǎng)因子121(VEGF121)單克隆抗體 | WB; IHC; ICC; IP. |
MAB851Hu24 | 血管內(nèi)皮生長(zhǎng)因子121(VEGF121)單克隆抗體 | WB; IHC; ICC; IP. |
MAB851Hu25 | 血管內(nèi)皮生長(zhǎng)因子121(VEGF121)單克隆抗體 | WB; IHC; ICC; IP. |
MAB851Hu27 | 血管內(nèi)皮生長(zhǎng)因子121(VEGF121)單克隆抗體 | WB; IHC; ICC; IP. |
MAB851Hu21 | 血管內(nèi)皮生長(zhǎng)因子121(VEGF121)單克隆抗體 | WB; IHC; ICC; IP. |
SEB851Hu | 血管內(nèi)皮生長(zhǎng)因子121(VEGF121)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB851Hu | 血管內(nèi)皮生長(zhǎng)因子121(VEGF121)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
PSB851Hu01 | 血管內(nèi)皮生長(zhǎng)因子121(VEGF121)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSB851Hu01 | 血管內(nèi)皮生長(zhǎng)因子121(VEGF121)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Oncogene | Elevated PLGF contributes to small-cell lung cancer brain metastasis [PubMed: 22797069] |
BMC?Cancer | VEGF-121 plasma level as biomarker for response to anti-angiogenetic therapy in recurrent glioblastoma [Pubmed:29747600] |
JOURNAL OF CLINICAL NEUROSCIENCE | A pediatric case of anaplastic astrocytoma with a gliomatosis cerebri; the growth pattern and changes in serum VEGF-121 levels after bevacizumab treatment [Pubmed: 33222957] |
INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE | Associations between VEGF isoforms and impending retinopathy of prematurity [Pubmed: 32737903] |
international journal of oncology | Activin A triggers angiogenesis via regulation of VEGFA and its overexpression is associated with poor prognosis of oral squamous cell carcinoma [Pubmed: 32377747] |
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