抗γ-氨基丁酸抗體(Anti-gABA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Anti-Gamma-Aminobutyric Acid Antibody (Anti-gABA)
4-Aminobutyric Acid
- 編號(hào)AEA900Ge
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法間接法
- 反應(yīng)時(shí)長(zhǎng)2h, 30min
- 檢測(cè)范圍3.12-200ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于1.18ng/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 3780 ¥ 5400 ¥ 24300 ¥ 45900 ¥ 378000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)抗γ-氨基丁酸抗體(Anti-gABA)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的抗γ-氨基丁酸抗體(Anti-gABA)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 95-105 | 101 |
| EDTA plasma(n=5) | 84-91 | 87 |
| heparin plasma(n=5) | 80-96 | 90 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的抗γ-氨基丁酸抗體(Anti-gABA)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中抗γ-氨基丁酸抗體(Anti-gABA)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-103% | 81-93% | 94-102% | 80-101% |
| EDTA plasma(n=5) | 91-102% | 92-99% | 80-95% | 92-101% |
| heparin plasma(n=5) | 86-93% | 92-101% | 96-103% | 87-103% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100μL，37°C孵育1小時(shí)；
4. 洗板5次；
5. 加TMB底物90µL，37°C孵育10-20分鐘；
6. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將抗原包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的抗γ-氨基丁酸抗體(Anti-gABA)與連接于固相載體上的抗原結(jié)合，然后加入HRP標(biāo)記的二抗，徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的抗γ-氨基丁酸抗體(Anti-gABA)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CPA900Ge11 | γ-氨基丁酸(gABA)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA900Ge21 | γ-氨基丁酸(gABA)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA900Ge41 | γ-氨基丁酸(gABA)人血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA900Ge01 | γ-氨基丁酸(gABA)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| PAA900Ge02 | γ-氨基丁酸(gABA)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA900Ge71 | γ-氨基丁酸(gABA)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA900Ge81 | γ-氨基丁酸(gABA)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| CEA900Ge | γ-氨基丁酸(gABA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA900Ge | 抗γ-氨基丁酸抗體(Anti-gABA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| LMA900Ge | γ-氨基丁酸(gABA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA900Ge11 | γ-氨基丁酸(gABA)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Toxicology and Industrial Health | Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellum [PubMed: 21444355] |
| Neurosci Lett | Expression of the neuron-specific potassium chloride cotransporter KCC2 in adult rat cochlear. [PubMed: 18577424] |
| Biomark Insights | Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism [PubMed: PMC3623607] |
| Biomarker insights | Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children [PubMed: PMC3694825] |
| Biomarker insights | Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) [PubMed: PMC3762604] |
| Scientific Reports | Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension [pubmed:27917928] |
| Journal of Neuroscience | Sex differences in the glutamate signaling pathway in juvenile rats [Pubmed:28861894] |
| Scientific Reports | A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzyme [Pubmed: 30341396] |
| Neurochemical?Research | Aucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of Neurotransmission [Pubmed: 30666488] |
| Neuroscience?Letters | Differentiation of human glioblastoma U87 cells into cholinergic neuron [Pubmed: 30928478] |
| Neurochemical Journal | Glutamate Signaling Defects in Propionic Acid Orally Administered to Juvenile Rats as an Experimental Animal Model of Autism [] |
| Effect of gamma radiation on combination therapy of certain antiepileptic drugs in rats. [] | |
| Experimental and Therapeutic Medicine | Antiepileptic effects of exogenous β?hydroxybutyrate on kainic acid?induced epilepsy [Pubmed: 33101467] |
| Exp Anim | Dementia model mice exhibited improvements of neuropsychiatric symptoms as well as cognitive dysfunction with neural cell transplantation [33828024] |
| Neurogenetics | Age-dependent neurological phenotypes in a mouse model of PRRT2-related diseases [34101060] |
| Sci Rep | Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission [34475508] |
| J Food Biochem | Adaptogenic potential of ginsenosides against domoic acid‐induced toxicity by regulating neuronal stress and kinate receptors: Ex vivo and in silico studies [Pubmed:35102569] |
