氧化低密度脂蛋白(OxLDL)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Oxidized Low Density Lipoprotein (OxLDL)
OL
- 編號CEA527Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競爭抑制
- 反應(yīng)時(shí)長2h
- 檢測范圍6.17-500ng/mL
- 靈敏度最小可檢測劑量小于等于2.59ng/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 3192 ¥ 4560 ¥ 20520 ¥ 38760 ¥ 319200
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測氧化低密度脂蛋白(OxLDL)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的氧化低密度脂蛋白(OxLDL)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 87-101 | 94 |
| EDTA plasma(n=5) | 85-99 | 90 |
| heparin plasma(n=5) | 79-101 | 98 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的氧化低密度脂蛋白(OxLDL)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中氧化低密度脂蛋白(OxLDL)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-98% | 96-105% | 91-101% | 80-99% |
| EDTA plasma(n=5) | 94-103% | 95-103% | 90-103% | 84-95% |
| heparin plasma(n=5) | 87-98% | 78-95% | 79-89% | 96-103% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時(shí)。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將氧化低密度脂蛋白(OxLDL)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計(jì)算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| SEA527Ra | 氧化低密度脂蛋白(OxLDL)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA527Ra | 氧化低密度脂蛋白(OxLDL)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA527Ra | 氧化低密度脂蛋白(OxLDL)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA527Ra | 氧化低密度脂蛋白(OxLDL)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA527Ra01 | 氧化低密度脂蛋白(OxLDL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Ethnopharmacology | Total panax notoginsenosides prevent atherosclerosis in apolipoprotein E-knockout mice: Role of downregulation of CD40 and MMP-9 expression [ScienceDirect: S0378874109005091] |
| Autonomic Neuroscience | Chemical sympathectomy induces arterial accumulation of native and oxidized LDL in hypercholesterolemic rats [ScienceDirect: S1566070211004152] |
| Cell Biochemistry and Biophysics | Plasma Paraoxonase-1, Oxidized Low-Density Lipoprotein and Lipid Peroxidation Levels in Gout Patients [SpringerLink: u466114p03434m6t] |
| Cardiovascular Research | Endothelial-specific Nox2 overexpression increases vascular superoxide and macrophage recruitment in ApoE-/- mice [PubMed: PMC3307381] |
| Atherosclerosis | 10-Dehydrogingerdione raises HDL-cholesterol through a CETP inhibition and wards off oxidation and inflammation in dyslipidemic rabbits. [Pubmed: 24267247] |
| PloS one | Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL. [NCBI: PMC3896367] |
| Atherosclerosis.? | Insulin-like growth factor I reduces lipid oxidation and foam cell formation via downregulation of 12/15-lipoxygenase [Pubmed:25549319] |
| J Surg Arts | THE FUNCTION OF SIALIC ACID AS A RADICAL SCAVENGER IN EXPERIMENTAL HYPOTHYROIDISM WITH AND WITHOUT HYPERLIPIDEMIA. [Cerrahisanatlardergisi:Source] |
| PLoS One. | Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL [Pubmed:24465540] |
| Saudi Med J | Increased systemic low-grade inflammation in high altitude native rats mediated by adrenergic receptors [Pubmed:24888651] |
| Vascular Medicine | Anti‐Inflammatory Immune Skewing Is Atheroprotective: Apoe?/?FcγRIIb?/? Mice Develop Fibrous Carotid Plaques [Pubmed:25516435] |
| Journal of Functional Foods | Oral administration of the β-glucan produced by?Aureobasidium pullulans?ameliorates development of atherosclerosis in apolipoprotein E deficient mice [Science: Article] |
| Acta Pharmacologica Sinica | Sirt1 is involved in decreased bone formation in aged apolipoprotein E-deficient mice [PubMed: 26592520] |
| Cancer Science | Suppressive effects of the NADPH oxidase inhibitor apocynin on intestinal tumorigenesis in obese KK‐Ay and Apc mutant Min mice [PubMed: 26310859] |
| BMJ Open Sport Exerc Med | Athletes with higher VO2max present reduced oxLDL after a marathon race [Content: 1] |
| Biochim Biophys Acta | PPARβ/δ ameliorates fructose-induced insulin resistance in adipocytes by preventing Nrf2 activation [PubMed: 25728706] |
| PLoS One | Ezetimibe Attenuates Atherosclerosis Associated with Lipid Reduction and Inflammation Inhibition [PubMed: 26555472] |
| PLoS One | Pulsatile Hyperglycaemia Induces Vascular Oxidative Stress and GLUT 1 Expression More Potently than Sustained Hyperglycaemia in Rats on High Fat Diet [Pubmed:26790104] |
| Diabetology & Metabolic Syndrome | Oxidative DNA damage and oxidized low density lipoprotein in Type II diabetes mellitus among patients with Helicobacter pylori infection [pmc:PMC4855822] |
| J Am Heart Assoc. | Transcriptional Profiling of Foam Cells Reveals Induction of Cavia (Guinea pig )anylate‐Binding Proteins Following Western Diet Acceleration of Atherosclerosis in the Absence of Global Changes in Inflammation [pmc:PMC4859273] |
| Journal of Applied Pharmaceutical Science | Organic Arsenical Exposure Stimulates Atherosclerosis through Oxidative Stress Increase and Adhesion Molecule Expression [admin:php] |
| Journal of Nutritional Biochemistry | Dietary rose hip exerts anti-atherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice [S0955286316301887] |
| Journal of?Basic?and?Clinical?Physiology and Pharmacology | Effects of altered thyroid states on oxidative stress parameters in rats [pubmed:27824612] |
| Journal of Functional Foods | Atherosclerosis-related inflammation and oxidative stress are improved by rice bran enzymatic extract [S1756464616302511] |
| Microvascular Research | Effects of insulin analogs as an add-on to metformin on cutaneous microcirculation in type 2 diabetic patients [pubmed:28954218] |
| Nutrients | Anti-Atherogenic Activity of Polyphenol-Rich Extract from Bee Pollen [pubmed:29258230] |
| Biochemical and Biophysical Research Communications | Oxidized low-density lipoprotein promotes osteoclast differentiation from CD68 positive mononuclear cells by regulating HMGB1 release [pubmed:29146189] |
| Journal of Surgical Research | THE FUNCTION OF SIALIC ACID AS A RADICAL SCAVENGER IN EXPERIMENTAL HYPOTHYROIDISM WITH AND WITHOUT HYPERLIPIDEMIA [10.14717/jsurgarts.2014.163] |
| biorxiv | Significant metabolic improvement by a water extract of olives: animal and human evidence [10.1101/151860] |
| Journal of nutritional biochemistry | Dietary rose hip exerts antiatherosclerotic effects and increases nitric oxide-mediated dilation in ApoE-null mice. [pubmed:28399420] |
| Nutrients | Yirui Capsules Alleviate Atherosclerosis by Improving the Lipid Profile and Reducing Inflammation in Apolipoprotein E-Deficient Mice [Pubmed:29382111] |
| Nutrition?&?Metabolism | Cold-pressed minke whale oil reduces circulating LDL/VLDL-cholesterol, lipid oxidation and atherogenesis in apolipoprotein E-deficient mice fed a Western?… [Pubmed:29755576] |
| Journal of Functional Foods | Saponins of sea cucumber attenuate atherosclerosis in ApoE?/? mice via lipid-lowering and anti-inflammatory properties [10.1016:j.jff.2018.07.046] |
| Journal of Agricultural and?Food?Chemistry | Neuroprotective effect of hydroxytyrosol in experimental diabetic retinopathy: relationship with cardiovascular biomarkers [Pubmed:29278909] |
| Molecules | Protective Effect of Polyphenol-Rich Extract from Bee Pollen in a High-Fat Diet [Pubmed:29614743] |
| Lipids in Health and Disease | Lipingshu capsule improves atherosclerosis associated with lipid regulation and inflammation inhibition in apolipoprotein E–deficient mice [Pubmed:30064511] |
| Diabetes Metabolism?Research?and Reviews? | Diabetic dyslipidaemia is associated with alterations in eNOS, caveolin‐1, and endothelial dysfunction in streptozotocin treated rats [Pubmed:29471582] |
| Diabetes & Metabolic Syndrome: Clinical Research & Reviews | Beyond LDL-c: The importance of serum oxidized LDL in predicting risk for type 2 diabetes in the middle-aged Asian Indians [Doi: 10.1016/j.dsx.2018.08.036] |
| 中國藥理學(xué)通報(bào) | 三七總皂苷對動(dòng)脈粥樣硬化小鼠的治療作用 [] |
| Biomedicine & Pharmacotherapy | Effects of lycopene on metabolism of glycolipid in type 2 diabetic rats [Pubmed: 30551463] |
| Journal of Ethnopharmacology | Anti-diabetic properties of different fractions of Korean red ginseng [] |
| Chinese Pharmacological Bulletin中國藥理學(xué)通報(bào) | 地昔帕明對兔動(dòng)脈斑塊細(xì)胞凋亡及內(nèi)質(zhì)網(wǎng)應(yīng)激的影響 [] |
| Document Version [] | |
| MEDICINE | Screening of schizophrenia associated miRNAs and the regulation of miR-320a-3p on integrin β1 [Pubmed: 30813134] |
| Pharmacological Research | Ethanol extracts of Danlou tablet attenuate atherosclerosis via inhibiting inflammation and promoting lipid effluent [Pubmed: 31181336] |
| Oxidative Medicine and Cellular Longevity | Combined Exercise Training Performed by Elderly Women Reduces Redox Indexes and Proinflammatory Cytokines Related to Atherogenesis [] |
| Nature Communications | ER-residential Nogo-B accelerates NAFLD-associated HCC mediated by metabolic reprogramming of oxLDL lipophagy [Pubmed: 31358770] |
| Clinical and Experimental Medicine | Senescence-associated miR-34a and miR-126 in middle-aged Indians with type 2 diabetes [Pubmed: 31732824] |
| Steroids | Serum 25 (OH) D concentration and its association with inflammation and oxidative stress in the middle-aged Indian healthy and diabetic subjects [Pubmed: 31672627] |
| ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Zygophyllum album saponins prevent atherogenic effect induced by deltamethrin via attenuating arterial accumulation of native and oxidized LDL in rats [Pubmed: 32105945] |
| JOURNAL OF CARDIOVASCULAR PHARMACOLOGY | Ginsenoside Rb1 Alleviates Oxidative Low-Density Lipoprotein–Induced Vascular Endothelium Senescence via the SIRT1/Beclin-1/Autophagy Axis [Pubmed: 31658172] |
| Journal of Diabetes Research | Middle-Aged Indians with Type 2 Diabetes Are at Higher Risk of Biological Ageing with Special Reference to Serum CDKN2A [Pubmed: 32280716] |
| Acta Biochim Pol | Dietary Lactobacillus plantarum LS/07 and inulin in the management of chronic disease risk factors [Pubmed: 33108141] |
| Ecotoxicol Environ Saf | Permethrin induced arterial retention of native and oxidized LDL in rats by promoting inflammation, oxidative stress and affecting LDL receptors, and collagen?… [Pubmed: 32911180] |
| Cell Death & Disease | Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells [Pubmed: 32796811] |
| Nutrients | Ginseng-Induced Changes to Blood Vessel Dilation and the Metabolome of Rats [Pubmed: 32727012] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | The Fungal Iron Chelator Desferricoprogen Inhibits Atherosclerotic Plaque Formation [Pubmed: 32635347] |
| Oxidative Medicine and Cellular Longevity | Improvement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based Proteomics [Pubmed: 32566106] |
| JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | The microvesicle/CD36 complex triggers a prothrombotic phenotype in patients with non‐valvular atrial fibrillation [Pubmed: 32510852] |
| Antioxidants | Nicotinamide Prevents Apolipoprotein B-Containing Lipoprotein Oxidation, Inflammation and Atherosclerosis in Apolipoprotein E-Deficient Mice [33233455] |
| MOLECULAR NUTRITION & FOOD RESEARCH | Digestive n?\6 Lipid Oxidation, a Key Trigger of Vascular Dysfunction and Atherosclerosis in the Western Diet: Protective Effects of Apple Polyphenols [33450108] |
| Scientific reports | Genetic deletion of Abcc6 disturbs cholesterol homeostasis in mice [33483533] |
| Scientific reports | Antibodies against apoB100 peptide 210 inhibit atherosclerosis in apoE-/-mice [33907226] |
| Journal of Food and Nutrition Research | Effect of Hibiscus sabdariffa Tea Extracts Intake on the Atherosclerosis Biomarkers in Adults [] |
| Journal of Food and Nutrition Research | Regulation of CD36 Gene Expression by Hibiscus Sabdariffa Tea Extracts to Affect the Atherosclerosis Biomarkers in Saudi Women [] |
| Eur J Nutr | The effects of long-term moderate exercise and Western-type diet on oxidative/nitrosative stress, serum lipids and cytokines in female Sprague Dawley rats [34319428] |
| Pediatr Gastroenterol Hepatol Nutr | The Relationship between Daily Fructose Consumption and Oxidized Low-Density Lipoprotein and Low-Density Lipoprotein Particle Size in Children with?… [34557400] |
| J Mol Cell Cardiol | ILF3 is responsible for hyperlipidemia-induced arteriosclerotic calcification by mediating BMP2 and STAT1 transcription [34343541] |
| J Basic Clin Physiol Pharmacol | Anti-atherosclerotic activity of aqueous extract of Ipomoea batatas (L.) leaves in high-fat diet-induced atherosclerosis model rats [Pubmed:34986543] |
| Oxid Med Cell Longev | DNA Methyltransferase 3b Accelerates the Process of Atherosclerosis [Pubmed:35422896] |
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