欧美综合自拍亚洲综合网_欧美黑人巨大videos异族_可乐操中文字幕_亚洲成av人福利专区网站_国产强奸视频在播放_免费无码国产在线53_最近最新的免費中文字幕_抖音免费视频素材_亚洲精品熟女一二区_国产无套粉嫩白浆在线麻豆

血管緊張素1-7(Ang1-7)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Angiotensin 1-7 (Ang1-7) ,etc. by FLIA (Flow Luminescence Immunoassay)

（注：單次混測多因子不超過8個指標 ）

  • 血管緊張素1-7(Ang1-7)等多因子檢測試劑盒(流式熒光發(fā)光法) 產品包裝(模擬)
  • 血管緊張素1-7(Ang1-7)等多因子檢測試劑盒(流式熒光發(fā)光法) 產品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測血管緊張素1-7(Ang1-7)等多因子檢測試劑盒(流式熒光發(fā)光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的血管緊張素1-7(Ang1-7)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 99-105 102
EDTA plasma(n=5) 80-95 86
heparin plasma(n=5) 98-105 101

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內加入適量的血管緊張素1-7(Ang1-7)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管緊張素1-7(Ang1-7)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 79-90% 88-101% 85-97% 79-101%
EDTA plasma(n=5) 78-89% 84-98% 85-103% 90-98%
heparin plasma(n=5) 87-96% 85-99% 79-101% 78-101%

穩(wěn)定性

經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠、檢測溶液A）標準品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
    37°C酶標板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將血管緊張素1-7(Ang1-7)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠、標記VEGFA，標記的血管緊張素1-7(Ang1-7)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的血管緊張素1-7(Ang1-7)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結合，然后將未結合物洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的血管緊張素1-7(Ang1-7)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關。

贈品

相關產品

編號 適用物種：Rattus norvegicus (Rat，大鼠) 應用(僅供研究使用，不用于臨床診斷！)
PAS085Ra01 血管緊張素1-7(Ang1-7)多克隆抗體 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAS085Ra21 血管緊張素1-7(Ang1-7)單克隆抗體 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
CES085Ra 血管緊張素1-7(Ang1-7)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMS085Ra 血管緊張素1-7(Ang1-7)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSS085Ra11 血管緊張素1-7(Ang1-7)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
J Stroke Cerebrovasc Dis Improvement of Plasma Biomarkers after Switching Stroke Patients from Other Angiotensin II Type I Receptor Blockers to Olmesartan [PubMed: 25891757]
Cellular Physiology and Biochemistry Angiotensin-(1-7) in Paraventricular Nucleus Contributes to the Enhanced Cardiac Sympathetic Afferent Reflex and Sympathetic Activity in Chronic Heart Failure Rats [pubmed:28848201]
Frontiers in Physiology Hydrogen Sulfide Attenuates Atherosclerosis in a Partially Ligated Carotid Artery Mouse model via Regulating Angiotensin Converting Enzyme 2 Expression [pubmed:29066981]
Molecular Medicine Reports The ACE2-Ang (1-7)-Mas receptor axis attenuates cardiac remodeling and fibrosis in post-myocardial infarction. [pubmed:28656296]
Journal of Clinical Pharmacy and Therapeutics Relationship between genetic variants of ACE2 gene and circulating levels of ACE2 and its metabolites. [pubmed:28895159]
Molecular Medicine Reports Effects of IQP, VEP and Spirulina platensis hydrolysates on the local kidney renin angiotensin system in spontaneously hypertensive rats [pubmed:28944898]
Open Journal of Endocrine and Metabolic Diseases Paper Submission A Study of the Correlation between Angiotensin (1-7) and the Histopathological Changes in the Penises of Experimentally Diabetic Rats [2-1980272_83198.htm]
Journal of the American Society of Hypertension Mesenchymal stem cell–derived microvesicles alleviate pulmonary arterial hypertension by regulating renin-angiotensin system [Pubmed:29752040]
Innate?Immunity? Lipoxin A attenuates LPS-induced acute lung injury via activation of the ACE2-Ang-(1-7)-Mas axis [Pubmed:29969931]
Evidence-based?Complementary and Alternative?Medicine? Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects [Pubmed:29977313]
European Journal of Obstetrics & Gynecology and Reproductive Biology The impact of ovulation inducing drugs on carcinogenesis []
European Journal of Obstetrics & Gynecology and Reproductive Biology Changes of angiotensin II (1–8) and angiotensin (1–7) levels in early and late-onset preeclampsia []
Journals of Gerontology Series A-Biological Sciences and Medical Sciences Therapeutic Delivery of Ang (1-7) Via Genetically Modified Probiotic: A Dosing Study. [Pubmed: 31586210]
journal of the european academy of dermatology and venereology Significance of the angiotensin I/angiotensin II/angiotensin‐(1‐7) axis in the pathogenesis of systemic sclerosis [Pubmed: 31746507]
Medicine Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study [Pubmed: 32756181]
Dikkat Eksikli?i Hiperaktivite Bozuklu?u Tan?s? Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin D?nü?türücü Enzim (ACE), NADPH Oksidaz Enzim Serum?… []
Amino Acids Angiotensin-(1¨C7) attenuates collagen-induced arthritis via inhibiting oxidative stress in rats [33398523]
Autoimmunity The prevalence and role of functional autoantibodies to angiotensin-converting-enzyme-2 in patients with systemic sclerosis [33910447]
researchsquare Ang-(1-7) Protects Skeletal Muscle Function in Aged Mice []
Peptides Angiotensin-(1-7) protects against sepsis-associated left ventricular dysfunction induced by lipopolysaccharide [34298021]
BMC Musculoskeletal Disorders Ang-(1–7) protects skeletal muscle function in aged mice [34548056]
J Cardiovasc Pharmacol Resveratrol Confers Protection Against Ischemia/Reperfusion Injury by Increase of Angiotensin (1-7) Expression in a Rat Model of Myocardial Hypertrophy [34232225]
留言咨詢