欧美综合自拍亚洲综合网_欧美黑人巨大videos异族_可乐操中文字幕_亚洲成av人福利专区网站_国产强奸视频在播放_免费无码国产在线53_最近最新的免費中文字幕_抖音免费视频素材_亚洲精品熟女一二区_国产无套粉嫩白浆在线麻豆

腦鈉素(BNP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Brain Natriuretic Peptide (BNP) ,etc. by FLIA (Flow Luminescence Immunoassay)

GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32

（注：?jiǎn)未位鞙y(cè)多因子不超過(guò)8個(gè)指標(biāo) ）

  • 腦鈉素(BNP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 腦鈉素(BNP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測(cè)腦鈉素(BNP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的腦鈉素(BNP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 93-102 97
EDTA plasma(n=5) 80-101 79
heparin plasma(n=5) 87-97 93

精密度

精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的腦鈉素(BNP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中腦鈉素(BNP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)含量的測(cè)定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 89-102% 78-101% 78-99% 98-105%
EDTA plasma(n=5) 97-104% 92-105% 89-96% 94-101%
heparin plasma(n=5) 90-104% 78-99% 97-104% 82-92%

穩(wěn)定性

經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
    37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測(cè)溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測(cè)溶液B100μL，37°C振動(dòng)孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實(shí)驗(yàn)原理

將腦鈉素(BNP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的腦鈉素(BNP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的腦鈉素(BNP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的腦鈉素(BNP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈(zèng)品

相關(guān)產(chǎn)品

編號(hào) 適用物種：Mus musculus (Mouse，小鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA541Mu01 腦鈉素(BNP)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA541Mu02 腦鈉素(BNP)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA541Mu21 腦鈉素(BNP)卵白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
PAA541Mu02 腦鈉素(BNP)多克隆抗體 WB; IHC; ICC; IP.
PAA541Mu01 腦鈉素(BNP)多克隆抗體 WB; IHC; ICC; IP.
PAA541Mu08 腦鈉素(BNP)多克隆抗體 WB; IHC; ICC; IP.
PAA541Mu03 腦鈉素(BNP)多克隆抗體 WB; IHC; ICC; IP.
LAA541Mu81 腦鈉素(BNP)多克隆抗體(異硫氰酸熒光素標(biāo)記) Applications: WB; IHC; ICC; IF.
LAA541Mu71 腦鈉素(BNP)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAA541Mu21 腦鈉素(BNP)單克隆抗體 WB; IHC; ICC; IP.
SEA541Mu 腦鈉素(BNP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA541Mu 腦鈉素(BNP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA541Mu 腦鈉素(BNP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA541Mu 腦鈉素(BNP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA541Mu11 腦鈉素(BNP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".
KSA541Mu01 腦鈉素(BNP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Heart and Circulatory Physiology B-type natriuretic peptide, vascular endothelial growth factor, endothelin-1, and nitric oxide synthase in chronic mountain sickness [APS: source]
PLoS One Renalase deficiency in heart failure model of rats_a potential mechanism underlying circulating norepinephrine accumulation. [PubMed: 21297953]
International Journal of Toxicology Cardiac Troponin T and NT-proBNP as Biomarkers of Early Myocardial Damage in Amitriptyline-Induced Cardiovascular Toxicity in Rats [Pubmed: 24055824]
JACC ELEVATED ST2 SERUM LEVELS A BIOMARKER FOR CARDIOMYOPATHY IN DUCHENNE MUSCULAR DYSTROPHY [Onlinejacc:Source]
J Urol.? Why does Adenotonsillectomy Not Correct Enuresis in All Children with Sleep Disordered Breathing? [Pubmed:24679871]
Life Sci Cardioprotective effects of baicalein on heart failure via modulation of Ca2 [PubMed: 26706290]
Vlaams Diergeneeskundig Tijdschrift Equine BNP measurement using a porcine BNP enzyme-linked immunoassay: a pilot study [Publication: 5950761]
Physiological Research Renal Sympathetic Denervation Improves Cardiac Dysfunction [PubMed: 25804096]
Life Sci Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitro [Pubmed:26706290]
J Ethnopharmacol Danhong injection attenuates isoproterenol-induced cardiac hypertrophy by reCavia (Guinea pig )lating p38 and NF-κb pathway [Pubmed:26970569]
Echocardiography. Improvement in the prediction of exercise-induced elevation of left ventricular filling pressure in patients with normal left ventricular ejection fraction. [pubmed:27779336]
SpringerPlus The effect of long-term amiodarone administration on myocardial fibrosis and evolution of left ventricular remodeling in a porcine model of ischemic … [pubmed:27652141]
Heart Lung Circ. Efficacy and Safety of Renal Sympathetic Denervation on Dogs with Pressure Overload-Induced Heart Failure. [pubmed:27555054]
Clinica?Chimica?Acta? Plasma concentration of fibronectin is decreased in patients with hypertrophic cardiomyopathy [pubmed:27693530]
Scientific Reports Paracrine cross-talk between skeletal muscle and macrophages in exercise by PGC-1α-controlled BNP. [pubmed:28091624]
Molecular Medicine Report Intermedin improves cardiac function and sympathetic neural remodeling in a rat model of post myocardial infarction heart failure [pubmed:28627670]
PLoS One Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy [pubmed:29240788]
Acta Physiologica Beneficial Effects of Renal Denervation on Cardiac Angiogenesis in Rats with Prolonged Pressure Overload [pubmed:27575955]
International Immunopharmacology Metformin attenuated endotoxin-induced acute myocarditis via activating AMPK [pubmed:28410530]
Drug Delivery Ginsenoside Rg3 micelles mitigate doxorubicin-induced cardiotoxicity and enhance its anticancer efficacy. [pubmed:29063791]
Science?of the?Total?Environment Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity and [Pubmed:29727991]
Scientific Reports An essential role for Wnt/β-catenin signaling in mediating hypertensive heart disease [Pubmed:29895976]
International Journal of Cardiology Cardioprotective effect of substance P in a porcine model of acute myocardial infarction [Pubmed:29884290]
International?Journal?of?Cardiology High-intensity interval training enhances mitochondrial bioenergetics of platelets in patients with heart failure [Pubmed:30072155]
Evidence-based?Complementary and Alternative?Medicine? Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects [Pubmed:29977313]
Molecular?Medicine?Reports? Hydrogen?rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats [Pubmed:29956781]
Pediatric Cardiology Determinants of Increased Aortic Diameters in Young Normotensive Patients With Turner Syndrome Without Structural Heart Disease [Pubmed:29392348]
BMC?Pharmacology and Toxicology Enoxaparin attenuates doxorubicin induced cardiotoxicity in rats via interfering with oxidative stress, inflammation and apoptosis [Pubmed:29321061]
中國(guó)病理生理雜志 和肽素水平對(duì)大鼠心腎綜合征的早期預(yù)測(cè)價(jià)值 [filename:ZBLS201802013]
FASEB?Journal Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure [Pubmed: 30230922]
Toxicology Mechanisms and?Methods Long-term exposure to crotonaldehyde causes heart and kidney dysfunction through induction of inflammatory and oxidative damage in male Wistar rats [Pubmed: 30426860]
Van Veterinary Journal Van Veterinary Journal []
Kidney?International Wnt/β-catenin signaling mediates both heart and kidney injury in type 2 cardiorenal syndrome [Pubmed: 30770217]
Journal?of?Cellular?Physiology Inflammatory cytokines associated with cancer growth induce mitochondria and cytoskeleton alterations in cardiomyocytes [Pubmed: 30982981]
Cardiology Circulating Connective Tissue Growth Factor Is Associated with Diastolic Dysfunction in Patients with Diastolic Heart Failure [Pubmed: 31466059]
BIOMEDICINE & PHARMACOTHERAPY Pharmacodynamic and pharmacokinetic effects of S086, a novel angiotensin receptor neprilysin inhibitor [Pubmed: 32570118]
biochimica et biophysica acta-molecular basis of disease Empagliflozin improves left ventricular diastolic function of db/db mice [Pubmed: 32353614]
Dapagliflozin: a Sodium-glucose Cotransporter 2 Inhibitor, Attenuates Angiotensin II-induced Cardiac Fibrotic Remodeling by Regulating TGF|?1/Smad ?- [34116674]
Radiation-induced heart energy metabolism dysfunction leads to heart fibrosis []
Oxid Med Cell Longev κ Opioid Receptor Agonist Inhibits Myocardial Injury in Heart Failure Rats through Activating Nrf2/HO-1 Pathway and Regulating Ca2+-SERCA2a [34373768]
Mol Med Rep Radiation?induced dysfunction of energy metabolism in the heart results in the fibrosis of cardiac tissues [34633055]
viruses Coxsackievirus A2 Leads to Heart Injury in a Neonatal Mouse Model [34452454]
Peptides Osteocrin alleviates cardiac hypertrophy via attenuating oxidative stress [Pubmed:35218795]
留言咨詢