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胰高血糖素樣肽1(GLP1)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Glucagon Like Peptide 1 (GLP1) ,etc. by FLIA (Flow Luminescence Immunoassay)

（注：單次混測多因子不超過8個(gè)指標(biāo) ）

  • 胰高血糖素樣肽1(GLP1)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 胰高血糖素樣肽1(GLP1)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒對檢測胰高血糖素樣肽1(GLP1)等多因子檢測試劑盒(流式熒光發(fā)光法)具有較高的靈敏度。
經(jīng)檢測人、小鼠、大鼠、兔、犬、牛之間有100%的交叉反映。

回收率

分別于定值血清及血漿樣本中加入一定量的胰高血糖素樣肽1(GLP1)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 91-101 98
EDTA plasma(n=5) 84-93 89
heparin plasma(n=5) 92-105 101

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的胰高血糖素樣肽1(GLP1)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中胰高血糖素樣肽1(GLP1)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 80-91% 78-101% 95-103% 96-105%
EDTA plasma(n=5) 91-98% 98-105% 86-97% 95-105%
heparin plasma(n=5) 93-101% 98-105% 78-101% 91-98%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠、檢測溶液A）標(biāo)準(zhǔn)品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
    37°C酶標(biāo)板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動(dòng)孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實(shí)驗(yàn)原理

將胰高血糖素樣肽1(GLP1)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠、標(biāo)記VEGFA，標(biāo)記的胰高血糖素樣肽1(GLP1)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標(biāo)記的胰高血糖素樣肽1(GLP1)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結(jié)合，然后將未結(jié)合物洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的胰高血糖素樣肽1(GLP1)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負(fù)相關(guān)。

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相關(guān)產(chǎn)品

編號 適用物種：Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Oryctolagus cuniculus (Rabbit，兔), Canis familiaris; Canine (Dog，犬), Bos taurus; Bovine (Cattle，牛) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
CPA804Mi22 胰高血糖素樣肽1(GLP1)卵白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
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PAA804Mi01 胰高血糖素樣肽1(GLP1)多克隆抗體 WB; IHC; ICC; IP.
MAA804Mi21 胰高血糖素樣肽1(GLP1)單克隆抗體 WB; IHC; ICC; IP.
MAA804Mi22 胰高血糖素樣肽1(GLP1)單克隆抗體 WB; IHC; ICC; IP.
MAA804Mi24 胰高血糖素樣肽1(GLP1)單克隆抗體 WB; IHC; ICC; IP.
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CEA804Mi 胰高血糖素樣肽1(GLP1)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA804Mi 胰高血糖素樣肽1(GLP1)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
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