皮質(zhì)酮(Cort)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)
Wide-range ELISA Kit for Corticosterone (Cort)
Corticosrone
- 編號WEA540Ge
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長3h
- 檢測范圍12.35-1,000ng/mL
- 靈敏度最小可檢測劑量小于等于5.13ng/mL.
- 樣本類型serum, plasma, urine and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3175 ¥ 4536 ¥ 20412 ¥ 38556 ¥ 317520
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測皮質(zhì)酮(Cort)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的皮質(zhì)酮(Cort)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-99 | 90 |
| EDTA plasma(n=5) | 81-92 | 88 |
| heparin plasma(n=5) | 87-97 | 93 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的皮質(zhì)酮(Cort)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中皮質(zhì)酮(Cort)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-96% | 81-102% | 88-95% | 87-102% |
| EDTA plasma(n=5) | 90-101% | 78-93% | 78-97% | 93-102% |
| heparin plasma(n=5) | 84-96% | 98-105% | 81-104% | 89-103% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質(zhì)水平。將皮質(zhì)酮(Cort)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本)，待測抗原與生物素標記抗原對特異性抗體進行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負相關(guān)。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Pan-species (General，通用) | 應用(僅供研究使用，不用于臨床診斷！) |
| CPA540Ge11 | 皮質(zhì)酮(Cort)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA540Ge21 | 皮質(zhì)酮(Cort)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA540Ge31 | 皮質(zhì)酮(Cort)鑰孔血藍蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA540Ge01 | 皮質(zhì)酮(Cort)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| PAA540Ge02 | 皮質(zhì)酮(Cort)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA540Ge71 | 皮質(zhì)酮(Cort)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAA540Ge81 | 皮質(zhì)酮(Cort)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAA540Ge28 | 皮質(zhì)酮(Cort)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA540Ge21 | 皮質(zhì)酮(Cort)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA540Ge22 | 皮質(zhì)酮(Cort)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA540Ge72 | 皮質(zhì)酮(Cort)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| CEA540Ge | 皮質(zhì)酮(Cort)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEA540Ge | 皮質(zhì)酮(Cort)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA540Ge | 皮質(zhì)酮(Cort)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA540Ge | 皮質(zhì)酮(Cort)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA540Ge | 皮質(zhì)酮(Cort)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA540Ge11 | 皮質(zhì)酮(Cort)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Respir Res. | Intracerebroventricular injection of leukotriene B4 attenuates antigen-induced asthmatic response via BLT1 receptor stimulating HPA-axis in sensitized rats. [PubMed: 20403205] |
| Natural Product Sciences | Effects of Gypenosides on Acute Stress in Mice [Papersearch: Source] |
| Neuropeptides.? | Role of adrenomedullin in the cerebrospinal fluid-contacting nucleus in the modulation of immobilization stress [Pubmed:25911494] |
| EXPERIMENTAL & CLINICAL CARDIOLOGY | Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in Mice [Com:Source] |
| Journal of Pharmacological Sciences | Organ-Specific Activation of the Gastric Branch of the Efferent Vagus Nerve by Ghrelin in Urethane-Anesthetized Rats [Pubmed:24366191] |
| Toxicology Letters | Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats [Pubmed:24440345] |
| Stress. | Restraint stress alters immune parameters and induces oxidative stress in the mouse uterus during embryo implantation [Pubmed:25238024] |
| Int J Clin Exp Med | Diabetes alters the blood glucose response to ketamine in streptozotocin-diabetic rats [PubMed: 26379948] |
| Pakistan Veterinary Journal | Trivalent Chromium Attenuated Corticosterone Secretion and Actions in Adrenocorticotropic Hormone-Stimulated Rats [In_Press: 15-058.Pdf] |
| BMC | Ameliorating effects of gypenosides on chronic stress-induced anxiety disorders in mice [PubMed: 26370834] |
| Behav Brain Res | Rats with differential self-grooming expression in the elevated plus-maze do not differ in anxiety-related behaviors [PubMed: 26142783] |
| Archives of Physiology and Biochemistry | Improvement of oral contraceptive-induced glucose dysreCavia (Guinea pig )lation and dyslipidemia by valproic acid is independent of circulating corticosterone [Pubmed:26855320] |
| Archives of Physiology and Biochemistry | Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder [Pubmed:26934364] |
| Neuroscience Letters | Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depression [Pubmed:26773866] |
| Neural Regeneration Research | Moxibustion?upreCavia (Guinea pig )lates?hippocampal?progranulin?expression. [Pubmed:27212922] |
| Environmental Science and Pollution Research | Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2/HO-1 pathway [Pubmed:27102619] |
| Eur Neuropsychopharmacol | Apelin-13 exerts antidepressant-like and recognition memory improving activities in stressed rats [Pubmed:26853763] |
| experimental brain research | Dopamine D2-like receptors modulate freezing response, but not the activation of HPA axis, during the expression of conditioned fear. [pubmed:27766352] |
| brain research bulletin | Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and oxidative stress in mouse hypothalamus. [pubmed:28161195] |
| Neurochemical?Research? | Effects?of (-)-Sesamin?on?Chronic?Stress-Induced?Anxiety?Disorders?in?Mice [pubmed:27995494] |
| Journal of Molecular Histology | Role of?serotonin in?the?intestinal mucosal epithelium barrier in?weaning mice undergoing stress-induced diarrhea [pubmed:29260435] |
| Psychosomatic Medicine | The Role of Psychological Stress on Heart Autophagy in Mice With Heart Failure [pubmed:28691995] |
| Scientific Reports COMMUNICATIONS | Environmental perturbation of the circadian clock during pregnancy leads to transgenerational mood disorder-like behaviors in mice [pubmed:28974783] |
| BMC Gastroenterology | Role of serotonin on the intestinal mucosal immune response to stress-induced diarrhea in weaning mice [pubmed:28633646] |
| biorxiv | Anxiety-like but not despair-like behaviors are further aggravated by chronic mild stress in the early stages of APPswe/PS1dE9 transgenic mice [doi.org/10.1101/202283] |
| Frontiers in Molecular Neuroscience | Rosiglitazone Exerts an Anti-depressive Effect in Unpredictable Chronic Mild-Stress-Induced Depressive Mice by Maintaining Essential Neuron Autophagy and Inhibiting Excessive Astrocytic Apoptosis. [pubmed:28959186] |
| Biochemical Pharmacology | Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in na?ve and Aβ-treated female rats [Pubmed:30028991] |
| Toxicological?Sciences | Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons [10.1093:toxsci] |
| Nutrition | Multigenerational effects of dietary macronutrients intake on the metabolic phenotype of male Wistar rats [10.1016:j.nut.2018.06.008] |
| Behavioural?Brain?Research | Depressive-and anxiety-like phenotypes in young adult APPSwe/PS1dE9 transgenic mice with insensitivity to chronic mild stress [Pubmed:30012417] |
| Stress | Blockade of AT1 type receptors for angiotensin II prevents cardiac microvascular fibrosis induced by chronic stress in Sprague–Dawley rats [Pubmed:29676198] |
| Inflammation | Differential Effects of Brain Death on Rat Microcirculation and Intestinal Inflammation: Female Male [Pubmed:29737476] |
| Respiratory physiology & neurobiology | Curcumin attenuates chronic intermittent hypoxia-induced brain injuries by inhibiting AQP4 and p38 MAPK pathway [Pubmed:29758366] |
| Nature and?Science?of?Sleep | Chronic treatment with dexamethasone alters clock gene expression and melatonin synthesis in rat pineal gland at night [Pubmed:30046256] |
| Journal?of?Pharmacy?and?Pharmacology | Tannins from fruits attenuates GABA antagonist‐induced anxiety‐like behaviour modulation of neurotransmitters [Pubmed: 30198561] |
| Bulletin?of Experimental?Biology?and?Medicine | Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in?… [Pubmed: 30353332] |
| Brain,?Behavior, and?Immunity | HMGB1-Mediated Differential Response on Hippocampal Neurotransmitter Disorder and Neuroinflammation in Adolescent Male and Female Mice following Cold Exposure [Pubmed: 30476565] |
| Heliyon | Establishment and evaluation of a novel mouse model of peri/postmenopausal depression [Doi: 10.1016/j.heliyon.2019.e01195] |
| Scientific Reports | Mice lacking Casp1, Ifngr and Nos2 genes exhibit altered depressive-and anxiety-like behaviour, and gut microbiome composition [Pubmed: 31015500] |
| Food & Function | Antidepressant-like effects of dietary gardenia blue pigment derived from genipin and tyrosine [Doi: 10.1039/C9FO00480G] |
| Neuroscience Letters | Synaptic structure and alterations in the hippocampus in neonatal rats exposed to lipopolysaccharide [Pubmed: 31288048] |
| FASEB Journal | Contribution of DNA methylation in chronic stress–induced cardiac remodeling and arrhythmias in mice [Pubmed: 31431066] |
| Cells | MicroRNA-99a is a Potential Target for Regulating Hypothalamic Synaptic Plasticity in the Peri/Postmenopausal Depression Model [Pubmed: 31540304] |
| endocrine journal | Mice with nucleus tractus solitarius injury induced by chronic restraint stress present impaired ability to raise blood glucose and glucagon levels when blood glucose?… [Pubmed: 32249244] |
| INTERNATIONAL IMMUNOPHARMACOLOGY | GABA-mediated activated microglia induce neuroinflammation in the hippocampus of mice following cold exposure through the NLRP3 inflammasome and?… [Pubmed: 33166810] |
| neurochemical research | Anxiolytic and Anti?depressive Like Effects of Translocator Protein (18 kDa) Ligand YL?IPA08 in a Rat Model of Postpartum Depression [Pubmed: 32383026] |
| BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488] |
| 博士論文 | Neurobiological determinants of depressive-like symptoms in rodents [] |
| Behavioral Ecology and Sociobiology? | Exposure to eagle owl feces induces anti-predator behavior, physiology, and hypothalamic gene responses in a subterranean rodent, the plateau zokor (Eospalax?… [] |
| Ther Adv Chronic Dis | Empagliflozin improves chronic hypercortisolism-induced abnormal myocardial structure and cardiac function in mice [33294147] |
| International Journal of?Nanomedicine | Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ?- [33762821] |
| CRYOBIOLOGY | O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress [33651993] |
| NEUROSCIENCE RESEARCH | Sleep disturbance induces depressive behaviors and neuroinflammation by altering the circadian oscillations of clock genes in rats [33785408] |
| Biomed Res Int | A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating?… [Pubmed:35281603] |
| Ecotoxicol Environ Saf | Monochromatic blue light not green light exposure is associated with continuous light-induced hepatic steatosis in high fat diet fed-mice via oxidative stress [Pubmed:35588616] |
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