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D-二聚體(D2D)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)

Wide-range ELISA Kit for D-Dimer (D2D)

D 2 Dimer

  • D-二聚體(D2D)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍) 產(chǎn)品包裝(模擬)
  • D-二聚體(D2D)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍) 產(chǎn)品包裝(模擬)
  • D-二聚體(D2D)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍) 實驗結(jié)果圖
  • WEA506Mu.jpg 標準曲線圖
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測D-二聚體(D2D)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的D-二聚體(D2D)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
sodium citrate plasma(n=5) 93-101 98

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的D-二聚體(D2D)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中D-二聚體(D2D)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
sodium citrate plasma(n=5) 83-94% 94-102% 95-103% 84-103%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）50µL，
    加入50µL檢測液A(臨用前配制);
    37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。

實驗原理

本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質(zhì)水平。將D-二聚體(D2D)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本)，待測抗原與生物素標記抗原對特異性抗體進行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負相關(guān)。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。

相關(guān)產(chǎn)品

編號 適用物種：Mus musculus (Mouse，小鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
CEA506Mu D-二聚體(D2D)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
WEA506Mu D-二聚體(D2D)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA506Mu D-二聚體(D2D)檢測試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA506Mu D-二聚體(D2D)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA506Mu11 D-二聚體(D2D)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
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Medical Science Monitor Impact of β-Adrenoceptor Blockade on Systemic Inflammation and Coagulation Disturbances in Rats with Acute Traumatic Coagulopathy [Pubmed:25676919]
Blood Intraperitoneal administration of activated protein C prevents postsurgical adhesion band formation. [PubMed: 25575539]
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