α1-抗胰蛋白酶(a1AT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Alpha-1-Antitrypsin (a1AT)
SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1
- 編號KSB697Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍62.5-4,000ng/mL
- 可適靈敏度29.2ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
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特異性
試劑盒中的抗體對α1-抗胰蛋白酶(a1AT)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
RPB697Hu01 | α1-抗胰蛋白酶(a1AT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB697Hu01 | α1-抗胰蛋白酶(a1AT)多克隆抗體 | WB; IHC; ICC; IP. |
LAB697Hu71 | α1-抗胰蛋白酶(a1AT)多克隆抗體(生物素標記) | WB; IHC; ICC. |
MAB697Hu24 | α1-抗胰蛋白酶(a1AT)單克隆抗體 | WB; IHC; ICC; IP. |
MAB697Hu25 | α1-抗胰蛋白酶(a1AT)單克隆抗體 | WB; IHC; ICC; IP. |
MAB697Hu23 | α1-抗胰蛋白酶(a1AT)單克隆抗體 | WB; IHC; ICC; IP. |
MAB697Hu29 | α1-抗胰蛋白酶(a1AT)單克隆抗體 | WB; IHC; ICC; IP. |
MAB697Hu27 | α1-抗胰蛋白酶(a1AT)單克隆抗體 | WB; IHC; ICC; IP. |
MAB697Hu26 | α1-抗胰蛋白酶(a1AT)單克隆抗體 | WB; IHC; ICC; IP. |
MAB697Hu21 | α1-抗胰蛋白酶(a1AT)單克隆抗體 | WB; IHC; ICC/IF. |
MAB697Hu22 | α1-抗胰蛋白酶(a1AT)單克隆抗體 | WB; IHC; ICC; IP. |
FAB697Hu02 | 抗α1-抗胰蛋白酶(a1AT)單克隆抗體 | Flow cytometry. |
MEB697Hu | α1-抗胰蛋白酶(a1AT)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEB697Hu | α1-抗胰蛋白酶(a1AT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCB697Hu | α1-抗胰蛋白酶(a1AT)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMB697Hu | α1-抗胰蛋白酶(a1AT)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB697Hu01 | α1-抗胰蛋白酶(a1AT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Journal of Biotechnology | Expression and characterization of recombinant human alpha-antitrypsin in transgenic rice seed [PubMed: 23376844] |
Sci Rep | Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis [PubMed: 26634430] |
PLoS One | Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study [PubMed: 25955845] |
Respir Care | Diagnostic Utility of Biomarkers in COPD [PubMed: 26106205] |
Acta?Anaesthesiologica Scandinavica | Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approach [Pubmed:29315472] |
Journal of Clinical Medicine | Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients [Pubmed: 30486327] |
Journal of Proteomics | Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection [Pubmed: 31706945] |
Diagnostics | Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848] |
Pediatrics International | Prenatal diagnosis of maternal serum from mothers carrying β‐thalassemic fetus [34559910] |
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