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α1-抗胰蛋白酶(a1AT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Alpha-1-Antitrypsin (a1AT)

SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1

  • α1-抗胰蛋白酶(a1AT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • α1-抗胰蛋白酶(a1AT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對α1-抗胰蛋白酶(a1AT)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關產(chǎn)品

編號 適用物種:Rattus norvegicus (Rat,大鼠) 應用(僅供研究使用,不用于臨床診斷!)
RPB697Ra01 α1-抗胰蛋白酶(a1AT)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB697Ra01 α1-抗胰蛋白酶(a1AT)多克隆抗體 WB; IHC; ICC; IP.
LAB697Ra71 α1-抗胰蛋白酶(a1AT)多克隆抗體(生物素標記) WB; IHC; ICC.
MAB697Ra21 α1-抗胰蛋白酶(a1AT)單克隆抗體 WB.
MEB697Ra α1-抗胰蛋白酶(a1AT)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
SEB697Ra α1-抗胰蛋白酶(a1AT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB697Ra α1-抗胰蛋白酶(a1AT)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSB697Ra01 α1-抗胰蛋白酶(a1AT)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
Journal of Biotechnology Expression and characterization of recombinant human alpha-antitrypsin in transgenic rice seed [PubMed: 23376844]
Sci Rep Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis [PubMed: 26634430]
PLoS One Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study [PubMed: 25955845]
Respir Care Diagnostic Utility of Biomarkers in COPD [PubMed: 26106205]
Acta?Anaesthesiologica Scandinavica Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approach [Pubmed:29315472]
Journal of Clinical Medicine Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients [Pubmed: 30486327]
Journal of Proteomics Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection [Pubmed: 31706945]
Diagnostics Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848]
Pediatrics International Prenatal diagnosis of maternal serum from mothers carrying β‐thalassemic fetus [34559910]
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