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血管生成素樣蛋白4(ANGPTL4)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Angiopoietin Like Protein 4 (ANGPTL4)

ARP4; FIAF; HFARP; NL2; PGAR; Pp1158; Fasting-Induced Adipose Factor; Hepatic Angiopoietin-Related Protein; Hepatic Fibrinogen/Angiopoietin-Related Protein

  • 血管生成素樣蛋白4(ANGPTL4)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 血管生成素樣蛋白4(ANGPTL4)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對血管生成素樣蛋白4(ANGPTL4)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPB019Hu02 血管生成素樣蛋白4(ANGPTL4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB019Hu01 血管生成素樣蛋白4(ANGPTL4)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB019Hu01 血管生成素樣蛋白4(ANGPTL4)多克隆抗體 WB; IHC; ICC; IP.
MAB019Hu22 血管生成素樣蛋白4(ANGPTL4)單克隆抗體 WB; IHC; ICC; IP.
SEB019Hu 血管生成素樣蛋白4(ANGPTL4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB019Hu 血管生成素樣蛋白4(ANGPTL4)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSB019Hu01 血管生成素樣蛋白4(ANGPTL4)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
The Journal of Biological Chemistry Angiopoietin-like 4 (Angptl4) Protein Is a Physiological Mediator of Intracellular Lipolysis in Murine Adipocytes [PubMed: PMC3318686]
J Clin Endocrinol Metab. Decreased Concentrations of the Lipoprotein Lipase Inhibitor Angiopoietin-Like Protein 4 and Increased Serum Triacylglycerol Are Associated With Increased Neonatal Fat Mass in Pregnant Women With Gestational Diabetes Mellitus [Pubmed: 23744407]
PloS one Lack of Relationship between Cord Serum Angiopoietin-Like Protein 4 (ANGPTL4) and Lipolytic Activity in Human Neonates Born by Spontaneous Delivery [Pubmed: 24324678]
Journal of Hematology & Oncology Angptl4 is upregulated under inflammatory conditions in the bone marrow of mice, expands myeloid progenitors, and accelerates reconstitution of platelets after myelosuppressive therapy [PubMed: 26054961]
Gynecol Endocrinol Examination of angiopoietin-like protein 4, neuropeptide Y, omentin-1 levels of obese and non-obese patients with polycystic ovary syndrome [PubMed: 26291814]
Molecular Nutrition & Food research Interaction of Gut Microbiota and High‐sodium, Low‐potassium Diet in Altering Plasma Triglyceride Profiles Revealed by Lipidomics Analysis [Pubmed: 31675161]
ANNALS OF AGRICULTURAL AND ENVIRONMENTAL MEDICINE Analysis of novel serum markers of fibrosis and angiogenesis in patients with alcoholic liver cirrhosis []
Food Chem Toxicol Di (2-ethylhexyl) phthalate exposure exacerbates metabolic disorders in diet-induced obese mice [34303773]
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