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鈣網(wǎng)蛋白(CALR)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Calreticulin (CALR)

CRT; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60

  • 鈣網(wǎng)蛋白(CALR)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 鈣網(wǎng)蛋白(CALR)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對鈣網(wǎng)蛋白(CRT)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

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參考文獻(xiàn)

雜志 參考文獻(xiàn)
Journal of Investigative Dermatology Oxidative Stress-Induced Calreticulin Expression and Translocation: New Insights into the Destruction of Melanocytes [Pubmed: 23771121]
International Journal of Molecular Sciences The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC [Mdpi: Source]
The Journal of Maternal-Fetal & Neonatal Medicine Amniotic fluid calreticulin in pregnancies complicated by the preterm prelabor rupture of membranes [Pubmed:26953684]
journal of maternal-fetal & neonatal medicine Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes. [pubmed:28152632]
Talanta Investigation of the interaction between calreticulin and immunoglobulin G by capillary electrophoresis and computer modeling [Doi: 10.1016/j.talanta.2018.11.098]
Sci Adv Thermostable ionizable lipid-like nanoparticle (iLAND) for RNAi treatment of hyperlipidemia [Pubmed:35171673]
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