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胱天蛋白酶8(CASP8)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Caspase 8 (CASP8)

CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog

  • 胱天蛋白酶8(CASP8)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 胱天蛋白酶8(CASP8)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

試劑盒中的抗體對胱天蛋白酶8(CASP8)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關產(chǎn)品

編號 適用物種:Mus musculus (Mouse,小鼠) 應用(僅供研究使用,不用于臨床診斷!)
RPA853Mu01 胱天蛋白酶8(CASP8)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA853Mu02 胱天蛋白酶8(CASP8)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA853Mu01 胱天蛋白酶8(CASP8)多克隆抗體 WB; IHC; ICC; IP.
PAA853Mu02 胱天蛋白酶8(CASP8)多克隆抗體 WB; IHC; ICC; IP.
LAA853Mu81 胱天蛋白酶8(CASP8)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
LAA853Mu71 胱天蛋白酶8(CASP8)多克隆抗體(生物素標記) WB; IHC; ICC.
SEA853Mu 胱天蛋白酶8(CASP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA853Mu 胱天蛋白酶8(CASP8)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA853Mu01 胱天蛋白酶8(CASP8)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 自己動手制作ELISA試劑盒的主要材料

參考文獻

雜志 參考文獻
Molecular Neurobiology Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations [Pubmed:25502462]
Journal of Ethnopharmacology Viscum articulatum Burm. f. aqueous extract exerts antiproliferative effect and induces cell cycle arrest and apoptosis in leukemia cells [Pubmed:29555410]
Clinical?And Experimental Pharmacology And Physiology? Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway [Pubmed:29266336]
Environmental Toxicology Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice [Pubmed: 31087429]
Environ Toxicol Pharmacol. Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats [Pubmed: 31421311]
Journal of Biochemical and Molecular Toxicology Tranilast attenuates methotrexate‐induced renal and hepatic toxicities: Role of apoptosis‐induced tissue proliferation [Pubmed: 32045101]
life sciences Nifuroxazide attenuates experimentally-induced hepatic encephalopathy and the associated hyperammonemia and cJNk/caspase-8/TRAIL activation in rats [Pubmed: 32259601]
Odontology Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells [Pubmed: 33206337]
Int Immunopharmacol Cardio-protective impact of gabapentin against doxorubicin-induced myocardial toxicity in rats; emphasis on modulation of inflammatory-apoptotic signaling [Pubmed: 33199237]
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY Chemo-preventive effect of crocin against experimentally-induced hepatocarcinogenesis via regulation of apoptotic and Nrf2 signaling pathways [Pubmed: 32942000]
Environ Sci Pollut Res Int Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low? dose of?… [Pubmed: 32915453]
J Biochem Mol Toxicol Tranilast abrogates cisplatin?\induced testicular and epididymal injuries: An insight into its modulatory impact on apoptosis/proliferation [34047436]
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