趨化素(CHEM)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Chemerin (CHEM)
TIG2; RARRES2; Tazarotene-Induced Gene 2; Retinoic Acid Receptor Responder 2,Tazarotene Induced; RAR-responsive protein TIG2
- 編號KSA945Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.069ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 6804 ¥ 11340 ¥ 20412 ¥ 39690 ¥ 68040
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特異性
試劑盒中的抗體對趨化素(CHEM)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
RPA945Hu01 | 趨化素(CHEM)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA945Hu01 | 趨化素(CHEM)多克隆抗體 | WB; IHC; ICC; IP. |
MAA945Hu21 | 趨化素(CHEM)單克隆抗體 | WB; IHC; ICC; IP. |
MAA945Hu23 | 趨化素(CHEM)單克隆抗體 | WB; IHC; ICC; IP. |
MAA945Hu22 | 趨化素(CHEM)單克隆抗體 | WB; IHC |
LAA945Hu54 | 趨化素(CHEM)單克隆抗體(別藻藍蛋白標記) | WB; IHC; ICC; IF. |
SEA945Hu | 趨化素(CHEM)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA945Hu | 趨化素(CHEM)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA945Hu01 | 趨化素(CHEM)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Scandinavian Journal of Gastroenterology | Serum chemerin and vaspin in non-alcoholic fatty liver disease [PubMed: 20095887] |
Gastroenterology & Hepatology | Chemerin, vaspin and insulin resistance in chronic hepatitis C [Wiley: source] |
Placenta | Expression and regulation of chemerin during rat pregnancy [ScienceDirect: S0143400412000902] |
Acta Pharmacologica Sinica | Chronic ethanol consumption increases the levels of chemerin in the serum and adipose tissue of humans and rats [Nature: 201211] |
RAED dergisi | Ankilozan spondilitli hastalarda adipositokin düzeyleri ve insülin direnci [Raeddergisi: Source] |
Journal of Cellular and Molecular Medicine | Association of chemerin with oxidative stress, inflammation and classical adipokines in non-diabetic obese patients [Pubmed: 24702860] |
Clinical Endocrinology | Strong correlations between circulating chemerin levels and lipoprotein subfractions in nondiabetic obese and nonobese subjects [Pubmed: 24303851] |
Oral Dis. | Impact of OSA on Biological Markers in Morbid Obesity and Metabolic Syndrome [Pubmed:24106991] |
Endocrine | Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsia [Pubmed:24840719] |
The Clinical Respiratory Journal | Relationship between plasma chemerin levels and disease severity in COPD patients [Pubmed:24865134] |
Gynecol Endocrinol. | Elevated serum chemerin in Chinese women with hyperandrogenic PCOS [Pubmed:24927079] |
Endocrine. | Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats. [Pubmed:25471281] |
Obesity Surgery | Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats [Pubmed:25697126] |
Dermatologica Sinica | Chemerin and calprotectin levels correlate with disease activity and inflammation markers in psoriasis vulgaris [Sciencedirect:S1027811714000470] |
Dis Markers | Serum Chemerin Levels in relation to Osteoporosis and Bone Mineral Density: A Case-Control Study [PubMed: 26170530] |
Med Sci Monit | Association of Serum Chemerin Levels with Acute Ischemic Stroke and Carotid Artery Atherosclerosis in a Chinese Population [PubMed: 26471865] |
Endocrine | Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats [PubMed: 25471281] |
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids | Effects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytes [Pubmed:26721419] |
Medicine (Baltimore). | Association?of?chemerin?levels?and?bone?mineral?density?in?Chinese?obese?postmenopausalwomen. [pubmed:27583869] |
Oxidative?Medicine?and?Cellular?Longevity | Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague?… [Pubmed:29849871] |
Current?Eye?Research | Chemerin is associated with diabetic retinopathy in type 2 diabetes [Pubmed:25848840] |
Journal of?Oral?Rehabilitation | Expression of chemerin in the synovial fluid of patients with temporomandibular joint disorders [Pubmed:29331039] |
International?Journal?of?Cancer | Epigenetic regulation of Amphiregulin and Epiregulin in colorectal cancer [Pubmed: 30252132] |
Experimental Gerontology | The decline of whole-body glucose metabolism in ovariectomized rats [Pubmed: 30292771] |
Pancreatology | Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-κB signal [Doi: 10.1016/j.pan.2019.02.005] |
Hypertens Pregnancy | Protective effect of vitamin D supplementation in a rat modal of preeclampsia: a possible implication of chemerin [Pubmed: 30922121] |
Pancreatology | Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-kB signal [Pubmed: 30833212] |
Digestion | Association between High Levels of Circulating Chemerin and Colorectal Adenoma in Men [Pubmed: 31269491] |
Vitamin D Supplementation Reduces Serum Chemerin Level in Gestational Diabetes Mellitus Rat Model [] | |
Aging Medicine | Plasma levels of adiponectin and chemerin are associated with early stage of atherosclerosis in older adults with type 2 diabetes mellitus [] |
Eur J Nutr | The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren [33575861] |
Eur J Pediatr | Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren [33834274] |
Indian Journal of Forensic Medicine & Toxicology | Altered Serum Marker of Adipokines Profile in Breast Cancer Women [] |
Int J Womens Health | Association of High Serum Chemerin with Bone Mineral Density Loss and Osteoporotic Fracture in Elderly Chinese Women [Pubmed:35140527] |
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