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趨化素(CHEM)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Chemerin (CHEM)

TIG2; RARRES2; Tazarotene-Induced Gene 2; Retinoic Acid Receptor Responder 2,Tazarotene Induced; RAR-responsive protein TIG2

  • 趨化素(CHEM)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 趨化素(CHEM)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)趨化素(CHEM)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。

配套試劑

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編號(hào) 適用物種：Mus musculus (Mouse，小鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
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參考文獻(xiàn)

雜志 參考文獻(xiàn)
Scandinavian Journal of Gastroenterology Serum chemerin and vaspin in non-alcoholic fatty liver disease [PubMed: 20095887]
Gastroenterology & Hepatology Chemerin, vaspin and insulin resistance in chronic hepatitis C [Wiley: source]
Placenta Expression and regulation of chemerin during rat pregnancy [ScienceDirect: S0143400412000902]
Acta Pharmacologica Sinica Chronic ethanol consumption increases the levels of chemerin in the serum and adipose tissue of humans and rats [Nature: 201211]
RAED dergisi Ankilozan spondilitli hastalarda adipositokin düzeyleri ve insülin direnci [Raeddergisi: Source]
Journal of Cellular and Molecular Medicine Association of chemerin with oxidative stress, inflammation and classical adipokines in non-diabetic obese patients [Pubmed: 24702860]
Clinical Endocrinology Strong correlations between circulating chemerin levels and lipoprotein subfractions in nondiabetic obese and nonobese subjects [Pubmed: 24303851]
Oral Dis. Impact of OSA on Biological Markers in Morbid Obesity and Metabolic Syndrome [Pubmed:24106991]
Endocrine Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsia [Pubmed:24840719]
The Clinical Respiratory Journal Relationship between plasma chemerin levels and disease severity in COPD patients [Pubmed:24865134]
Gynecol Endocrinol. Elevated serum chemerin in Chinese women with hyperandrogenic PCOS [Pubmed:24927079]
Endocrine. Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats. [Pubmed:25471281]
Obesity Surgery Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats [Pubmed:25697126]
Dermatologica Sinica Chemerin and calprotectin levels correlate with disease activity and inflammation markers in psoriasis vulgaris [Sciencedirect:S1027811714000470]
Dis Markers Serum Chemerin Levels in relation to Osteoporosis and Bone Mineral Density: A Case-Control Study [PubMed: 26170530]
Med Sci Monit Association of Serum Chemerin Levels with Acute Ischemic Stroke and Carotid Artery Atherosclerosis in a Chinese Population [PubMed: 26471865]
Endocrine Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats [PubMed: 25471281]
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids Effects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytes [Pubmed:26721419]
Medicine (Baltimore). Association?of?chemerin?levels?and?bone?mineral?density?in?Chinese?obese?postmenopausalwomen. [pubmed:27583869]
Oxidative?Medicine?and?Cellular?Longevity Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague?… [Pubmed:29849871]
Current?Eye?Research Chemerin is associated with diabetic retinopathy in type 2 diabetes [Pubmed:25848840]
Journal of?Oral?Rehabilitation Expression of chemerin in the synovial fluid of patients with temporomandibular joint disorders [Pubmed:29331039]
International?Journal?of?Cancer Epigenetic regulation of Amphiregulin and Epiregulin in colorectal cancer [Pubmed: 30252132]
Experimental Gerontology The decline of whole-body glucose metabolism in ovariectomized rats [Pubmed: 30292771]
Pancreatology Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-κB signal [Doi: 10.1016/j.pan.2019.02.005]
Hypertens Pregnancy Protective effect of vitamin D supplementation in a rat modal of preeclampsia: a possible implication of chemerin [Pubmed: 30922121]
Pancreatology Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-kB signal [Pubmed: 30833212]
Digestion Association between High Levels of Circulating Chemerin and Colorectal Adenoma in Men [Pubmed: 31269491]
Vitamin D Supplementation Reduces Serum Chemerin Level in Gestational Diabetes Mellitus Rat Model []
Aging Medicine Plasma levels of adiponectin and chemerin are associated with early stage of atherosclerosis in older adults with type 2 diabetes mellitus []
Eur J Nutr The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren [33575861]
Eur J Pediatr Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren [33834274]
Indian Journal of Forensic Medicine & Toxicology Altered Serum Marker of Adipokines Profile in Breast Cancer Women []
Int J Womens Health Association of High Serum Chemerin with Bone Mineral Density Loss and Osteoporotic Fracture in Elderly Chinese Women [Pubmed:35140527]
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