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補(bǔ)體因子D(CFD)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Complement Factor D (CFD)

CF-D; DF; PFD; ADN; Adipsin; Properdin Factor D; C3 convertase activator

  • 補(bǔ)體因子D(CFD)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 補(bǔ)體因子D(CFD)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)補(bǔ)體因子D(CFD)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。

配套試劑

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參考文獻(xiàn)

雜志 參考文獻(xiàn)
Journal of?Diabetes?Investigation Relationship between serum adipsin and the first phase of glucose‐stimulated insulin secretion in individuals with different glucose tolerance [Pubmed:29432659]
Endocrine?Practice CORRELATION OF INCREASED SERUM ADIPSIN WITH INCREASED CARDIOVASCULAR RISKS IN ADULT PATIENTS WITH GROWTH HORMONE DEFICIENCY [Pubmed: 30657359]
BMC Endocrine Disorders Association between plasma adipsin level and mild cognitive impairment in Chinese patients with type 2 diabetes: a cross-sectional study [Pubmed: 31651303]
Scientific Reports HFD-induced hepatic lipid accumulation and inflammation are decreased in Factor D deficient mouse [Pubmed: 33067533]
Biomedicine & Pharmacotherapy Body fat distribution and circulating adipsin are related to metabolic risks in adult patients with newly diagnosed growth hormone deficiency and improve after?… [Pubmed: 33254428]
Nephrol Dial Transplant Association between thrombotic microangiopathy and activated alternative complement pathway in malignant nephrosclerosis [33367879]
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