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親環(huán)素A(CYPA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Cyclophilin A (CYPA)

PPIA; CyP-A; CYPH; Peptidylprolyl Isomerase A; Peptidyl-Prolyl Isomerase A; Peptidyl-prolyl cis-trans isomerase A; Cyclosporin A-binding protein

  • 親環(huán)素A(CYPA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 親環(huán)素A(CYPA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對親環(huán)素A(CYPA)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

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編號 適用物種:Mus musculus (Mouse,小鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
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參考文獻(xiàn)

雜志 參考文獻(xiàn)
Atherosclerosis EMMPRIN and its ligand cyclophilin A regulate MT1-MMP, MMP-9 and M-CSF during foam cell formation [PubMed: 19758589]
Biomarkers Cyclophilin-A: a novel biomarker for untreated male essential hypertension [Pubmed: 24164594]
Cardiovascular Diabetology Plasma level of cyclophilin A is increased in patients with type 2 diabetes mellitus and suggests presence of vascular disease [Pubmed:24502618]
International Journal of Biological Sciences Decrease in Plasma Cyclophilin A Concentration at 1 Month after Myocardial Infarction Predicts Better Left Ventricular Performance and Synchronicity at 6 Months: A Pilot Study in Patients with ST Elevation Myocardial Infarction [Pubmed:25552928]
Medicine Cyclophilin A in Ruptured Intracranial Aneurysm [PubMed: 26426668]
Mol Cell Biochem PPIA rs6850: A> G single-nucleotide polymorphism is associated with raised plasma cyclophilin A levels in patients with coronary artery disease [PubMed: 26702934]
Medicine (Baltimore) Urinary Cyclophilin A as a New Marker for Diabetic Nephropathy: A Cross-Sectional Analysis of Diabetes Mellitus [PubMed: 26496315]
International Journal of Cardiology Cyclophilin?A?modulates?bone?marrow-derived?CD117(+)?cells?and?enhances?ischemia-induced?angiogenesisvia?the?SDF-1/CXCR4?axis. [Pubmed:27057951]
Clinica?Chimica?Acta Novel findings of secreted cyclophilin A in diabetic nephropathy and its association with renal protection of dipeptidyl peptidase 4 inhibitor [pubmed:27823952]
Cardiovascular?Diabetology Cyclophilin?A?enhances?macrophage?differentiation?and?lipid?uptake?in?high?glucose?conditions: a cellular mechanism for accelerated macro vascular disease in diabetes mellitus. [pubmed:27809851]
Clinical?Science Metformin attenuates effects of cyclophilin A on macrophages, reduces lipid uptake and secretion of cytokines by repressing decreased AMPK activity [Pubmed:29382697]
Journal of Molecular Histology Cyclophilin a increases CD68 cell infiltration in rat experimental periodontitis [Pubmed:29318411]
Free?Radical?Research Cyclophilin A and CD147 associate with progression of diabetic nephropathy [Pubmed: 30572748]
The Kaohsiung Journal of Medical Sciences Association of cyclophilin A level and pulse pressure in predicting recurrence of cerebral infarction [Pubmed: 31670477]
Cells. Cyclophilin A/EMMPRIN Axis Is Involved in Pro-Fibrotic Processes Associated with Thoracic Aortic Aneurysm of Marfan Syndrome Patients [Pubmed: 31936351]
JAMA Neurology APPENDIX A: Relationship between cyclophilin A levels and matrix metalloproteinase-9 activity in cerebrospinal fluid of cognitively normal apolipoprotein E4 carriers?… [Pubmed: 24030206]
Menoufia Medical Journal Urinary podocalyxin and cyclophilin A: markers for early detection of type 2 diabetic nephropathy []
Toxins Evaluation of the Therapeutic Effects of Protocatechuic Aldehyde in Diabetic Nephropathy [34437430]
Cell Death & Disease Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell death [Pubmed:35732625]
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