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內皮糖蛋白(ENG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Endoglin (ENG)

CD105; END; HHT1; ORW; ORW1; Osler-Rendu-Weber Syndrome 1

  • 內皮糖蛋白(ENG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產品包裝(模擬)
  • 內皮糖蛋白(ENG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

試劑盒中的抗體對內皮糖蛋白(ENG)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

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參考文獻

雜志 參考文獻
Clin Rev Allergy Immunol Endothelial Dysfunction and Nailfold Videocapillaroscopy Pattern as Predictors of Digital Ulcers in Systemic Sclerosis: a Cohort Study and Review of the Literature [PubMed: 26142066]
Angiologia e Cirurgia Vascular Peripheral vasculopathy in Raynaud phenomenon: Vascular disease biomarkers [science:S1646706X16300052]
Clinical Rheumatology Impaired angiogenesis as a feature of digital ulcers in systemic sclerosis [Pubmed:26920752]
Advanced?Biomedical?Research Diagnostic and prognostic significance of serum soluble endoglin levels in preeclampsia and eclampsia [pubmed:27563629]
Annals of agricultural and environmental medicine Relationships between serum selenium and zinc concentrations versus profibrotic and proangiogenic cytokines (FGF-19 and endoglin) in patients with alcoholic liver cirrhosis. [pubmed:28954507]
ResearchSquare The predictive role of PlGF/sENG ratio in fetal growth restriction of early beginning associated with pre-eclampsia []
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