凝溶膠蛋白(GSN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Gelsolin (GSN)
GS; AGEL; ADF; Brevin; Gelsolin Amyloidosis,Finnish Type; Actin-depolymerizing factor
- 編號KSA372Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍62.5-4000pg/mL
- 可適靈敏度28.2pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 6048 ¥ 10080 ¥ 18144 ¥ 35280 ¥ 60480
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特異性
試劑盒中的抗體對凝溶膠蛋白(GS)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA372Hu02 | 凝溶膠蛋白(GSN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA372Hu05 | 凝溶膠蛋白(GSN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA372Hu61 | 凝溶膠蛋白(GSN)活性蛋白 | Cell?culture;?Activity?Assays. |
RPA372Hu06 | 凝溶膠蛋白(GSN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA372Hu03 | 凝溶膠蛋白(GSN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA372Hu04 | 凝溶膠蛋白(GSN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA372Hu01 | 凝溶膠蛋白(GSN)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPA372Hu61 | 凝溶膠蛋白(GSN)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
CPA372Hu21 | 凝溶膠蛋白(GSN)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAA372Hu05 | 凝溶膠蛋白(GSN)多克隆抗體 | WB; IHC; ICC; IP. |
PAA372Hu04 | 凝溶膠蛋白(GSN)多克隆抗體 | WB; IHC; ICC; IP. |
PAA372Hu06 | 凝溶膠蛋白(GSN)多克隆抗體 | WB; IHC; ICC; IP. |
PAA372Hu03 | 凝溶膠蛋白(GSN)多克隆抗體 | WB; IHC; ICC; IP. |
PAA372Hu02 | 凝溶膠蛋白(GSN)多克隆抗體 | WB; IHC; ICC; IP. |
PAA372Hu01 | 凝溶膠蛋白(GSN)多克隆抗體 | WB; IHC; ICC; IP. |
PAA372Hu08 | 凝溶膠蛋白(GSN)多克隆抗體 | WB; IHC; ICC; IP. |
LAA372Hu71 | 凝溶膠蛋白(GSN)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAA372Hu27 | 凝溶膠蛋白(GSN)單克隆抗體 | WB; IHC; ICC; IP. |
MAA372Hu29 | 凝溶膠蛋白(GSN)單克隆抗體 | WB; IHC; ICC; IP. |
MAA372Hu22 | 凝溶膠蛋白(GSN)單克隆抗體 | WB; IHC; ICC; IP. |
MAA372Hu24 | 凝溶膠蛋白(GSN)單克隆抗體 | WB; IHC |
MAA372Hu21 | 凝溶膠蛋白(GSN)單克隆抗體 | WB; IHC; ICC; IP. |
MAA372Hu23 | 凝溶膠蛋白(GSN)單克隆抗體 | WB; IHC; ICC; IP. |
MAA372Hu25 | 凝溶膠蛋白(GSN)單克隆抗體 | WB; IHC; ICC; IP. |
MAA372Hu26 | 凝溶膠蛋白(GSN)單克隆抗體 | WB; IHC; ICC; IP. |
SEA372Hu | 凝溶膠蛋白(GSN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
WEA372Hu | 凝溶膠蛋白(GSN)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA372Hu | 凝溶膠蛋白(GSN)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA372Hu | 凝溶膠蛋白(GSN)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
PSA372Hu01 | 凝溶膠蛋白(GSN)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA372Hu01 | 凝溶膠蛋白(GSN)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Neurochmical Research | Gelsolin in Cerebrospinal Fluid as a Potential Biomarker of Epilepsy [SpringerLink: 3585r8h46q7r0171] |
Journal of Neuroinflammation | Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injury [BioMed: 17422094] |
Biomedical Engineering and Informatics | Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancer [IEEE: 6098563] |
European Journal of Obstetrics & Gynecology and Reproductive Biology | Investigation of the actin scavenging system in pre-eclampsia [Pubmed: 24239294] |
The Annals of Thoracic Surgery | Proteomic Study Reveals Plasma Protein Changes in Congenital Heart Diseases [Pubmed: 24565402] |
BMC Anesthesiol.? | The role of plasma gelsolin in cardiopulmonary bypass induced acute lung injury in infants and young children: a pilot study [Pubmed:Pmc4132929] |
Cell Biochemistry and Biophysics | Gelsolin: Role of a Functional Protein in Mitigating Radiation Injury [Pubmed:25164111] |
Neurosci Lett | Plasma gelsolin and matrix metalloproteinase 3 as potential biomarkers for Alzheimer disease [PubMed: 25864780] |
Proteomics Clinical Applications | Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients [Pubmed:26680509] |
PROTEOMICS?-?Clinical?Applications | Comparative mass spectrometric and immunoassay-based?proteome?analysis?in?serum?ofDuchenne muscular dystrophy?patients. [pubmed:26680509] |
Medicina Intensiva | Serum gelsolin levels in aneurismal subarachnoid hemorrhage: Preliminary results. [pubmed:28365030] |
Journal of?Alzheimers?Disease | Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods [Pubmed:30040720] |
World?Journal of Pediatrics | Plasma gelsolin level predicts acute kidney injury after cardiopulmonary bypass in infants and young children [Pubmed:29427164] |
Biomedicines | Production and Secretion of Gelsolin by Both Human Macrophage-and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various?… [Pubmed:35327525] |
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