高爾基磷酸化蛋白2(GOLPH2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Golgi Phosphoprotein 2 (GOLPH2)
GOLM1; GP73; C9orf155; Golgi Membrane Protein 1; Golgi Protein 73
- 編號KSB668Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.94-60ng/mL
- 可適靈敏度0.37ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對高爾基蛋白73(GP73)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB668Mu02 | 高爾基磷酸化蛋白2(GOLPH2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB668Mu01 | 高爾基磷酸化蛋白2(GOLPH2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB668Mu01 | 高爾基磷酸化蛋白2(GOLPH2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB668Mu02 | 高爾基磷酸化蛋白2(GOLPH2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB668Mu01 | 高爾基磷酸化蛋白2(GOLPH2)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| LAB668Mu81 | 高爾基磷酸化蛋白2(GOLPH2)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAB668Mu71 | 高爾基磷酸化蛋白2(GOLPH2)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEB668Mu | 高爾基磷酸化蛋白2(GOLPH2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB668Mu | 高爾基磷酸化蛋白2(GOLPH2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB668Mu01 | 高爾基磷酸化蛋白2(GOLPH2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Molecular & Cellular Proteomics | LAMC2: A promising new pancreatic cancer biomarker identified by proteomic analysis of pancreatic adenocarcinoma tissues [Mcponline: 023507] |
| Afro-Egypt J Infect Endem Dis | Diagnostic and Prognostic Validity of Serum Golgi Protein 73 in Egyptian Patients with Hepatocelluar Carcinoma [Ajied: Ajied_System_Files] |
| Journal of Applied Pharmaceutical Science | Diagnostic role of Golgi protein 73 and IL-17 in Egyptian cirrhotic rather than hepatocellular carcinoma patients. [handle:123456789] |
| Oncology Letters | Serum Golgi protein 73 is a prognostic rather than diagnostic marker in hepatocellular carcinoma [pubmed:29113278] |
| Scientific Reports | The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study [Pubmed: 32198440] |
| Hepatobiliary & Pancreatic Diseases International | Dynamic expression of hepatic GP73 mRNA and protein and circulating GP73 during hepatocytes malignant transformation [Pubmed: 32171652] |
| Biomolecules | Deoxycholic Acid Upregulates Serum Golgi Protein 73 through Activating NF-|êB Pathway and Destroying Golgi Structure in Liver Disease. Biomolecules 2021, 11??- [33540642] |
| ADVANCED RESEARCHES | Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C [] |
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