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70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Heat Shock 70kDa Protein 1A (HSPA1A)

HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70

  • 70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對70kDa熱休克蛋白1A(HSPA1A)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關(guān)產(chǎn)品

編號 適用物種:Bos taurus; Bovine (Cattle,牛) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPB081Bo01 70kDa熱休克蛋白1A(HSPA1A)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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LAB081Bo71 70kDa熱休克蛋白1A(HSPA1A)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAB081Bo21 70kDa熱休克蛋白1A(HSPA1A)單克隆抗體 WB; IHC; ICC; IP.
SEB081Bo 70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB081Bo 70kDa熱休克蛋白1A(HSPA1A)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSB081Bo01 70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Journal of Neuroinflammation Identification of potential biomarkers of gold nanoparticle toxicity in rat brains [PubMed: 22691312]
Mediators Inflamm.? Local Inflammation in Fracture Hematoma: Results from a Combined Trauma Model in Pigs [Pubmed:Pmc4324980]
Eur J Pharmacol. Differential modulatory effects of morphine on acute and chronic stress induced neurobehavioral and cellular markers in rats [Pubmed:24508522]
Pharmacol Rep. Effect of propranolol on IL-10, visfatin, Hsp70, iNOS, TLR2, and survivin in amelioration of tumor progression and survival in Solid Ehrlich Carcinoma-bearing mice [Pubmed:25443743]
Advances in Medical Sciences A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics [pubmed:28822267]
Archives of Medical Research Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young?… [Pubmed:29379533]
metabolic brain disease Curcumin attenuates cytoplasmic/endoplasmic reticulum stress, apoptosis and cholinergic dysfunction in diabetic rat hippocampus [Pubmed: 32172517]
EXPERIMENTAL CELL RESEARCH Antagomirs targeting miR-142–5p attenuate pilocarpine-induced status epilepticus in mice [Pubmed: 32439493]
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