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血幼素(HJV)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Hemojuvelin (HJV)

HFE2; HFE2A; HJV; JH; RGMC; hemochromatosis type 2(Juvenile); Haemojuvelin; RGM domain family member C

  • 血幼素(HJV)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 血幼素(HJV)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)血幼素 (HJV)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào) 適用物種:Rattus norvegicus (Rat,大鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPB995Ra01 血幼素(HJV)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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LMB995Ra 血幼素(HJV)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
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參考文獻(xiàn)

雜志 參考文獻(xiàn)
PLoS One. Effect of Iron Overload and Iron Deficiency on Liver Hemojuvelin Protein [PubMed: PMC3356351]
Journal of Hepcidin Levels Are Increased in Patients with Acute Ischemic Stroke: Preliminary Report [PubMed: 25881778]
European Journal of Haematology Soluble hemojuvelin in transfused and untransfused thalassaemic subjects [doi:10.1111]
American Journal of Nephrology The secret war against counterfeit science [pubmed:28492262]
Critical?Care Urinary biomarkers predict advanced acute kidney injury after cardiovascular surgery [Pubmed:29699579]
Scientific Reports Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac Surgery [Pubmed:29386545]
Journal of Cerebral Blood Flow and Metabolism The functional role of hemojuvelin in acute ischemic stroke [Pubmed: 31307288]
Oxidative damage and iron dyshomeostasis in thalassemic patients []
J Trace Elem Med Biol Caffeine and alcohol-friends or foes of human iron stores? [Pubmed:35063815]
Arch Pathol Lab Med Urinary Biomarkers Can Predict Weaning From Acute Dialysis Therapy in Critically Ill Patients [Pubmed:35311928]
Arch Pathol Lab Med The Relationships among Plasma Fetuin-B, Thyroid Autoimmunity, and Fertilization Rate In Vitro Fertilization and Embryo Transfer [Pubmed:35340679]
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