肝細(xì)胞生長(zhǎng)因子(HGF)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Hepatocyte Growth Factor (HGF)
HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen
- 編號(hào)KSA047Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍31.2-2000pg/mL
- 可適靈敏度12.3pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 4914 ¥ 8190 ¥ 14742 ¥ 28665 ¥ 49140
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)肝細(xì)胞生長(zhǎng)因子(HGF)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA047Hu02 | 肝細(xì)胞生長(zhǎng)因子(HGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA047Hu03 | 肝細(xì)胞生長(zhǎng)因子(HGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA047Hu01 | 肝細(xì)胞生長(zhǎng)因子(HGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA047Hu01 | 肝細(xì)胞生長(zhǎng)因子(HGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA047Hu02 | 肝細(xì)胞生長(zhǎng)因子(HGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA047Hu01 | 肝細(xì)胞生長(zhǎng)因子(HGF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA047Hu02 | 肝細(xì)胞生長(zhǎng)因子(HGF)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA047Hu03 | 肝細(xì)胞生長(zhǎng)因子(HGF)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA047Hu71 | 肝細(xì)胞生長(zhǎng)因子(HGF)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA047Hu53 | 肝細(xì)胞生長(zhǎng)因子(HGF)多克隆抗體(別藻藍(lán)蛋白標(biāo)記) | WB; IHC; ICC; IF. |
| LAA047Hu81 | 肝細(xì)胞生長(zhǎng)因子(HGF)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAA047Hu22 | 肝細(xì)胞生長(zhǎng)因子(HGF)單克隆抗體 | WB; IHC |
| RAA047Hu21 | 肝細(xì)胞生長(zhǎng)因子(HGF)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MAA047Hu23 | 肝細(xì)胞生長(zhǎng)因子(HGF)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA047Hu | 肝細(xì)胞生長(zhǎng)因子(HGF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA047Hu | 肝細(xì)胞生長(zhǎng)因子(HGF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA047Hu | 肝細(xì)胞生長(zhǎng)因子(HGF)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA047Hu | 肝細(xì)胞生長(zhǎng)因子(HGF)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA047Hu01 | 肝細(xì)胞生長(zhǎng)因子(HGF)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Gene Therapy and Molecular Biology | Sphingosine kinase-1 protects transplanted mesenchymal stem cells and improves the performance of the infarcted heart [LWW: 01000] |
| Journal of Translational Medicine | Low temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinoma [BioMed: 14795876] |
| Digestive Diseases and Sciences | Gene Transfer of c-met Confers Protection Against d-Galactosamine/Lipopolysaccharide-Induced Acute Liver Failure [SpringerLink: k22823l138x2344x] |
| Biomarker insights | Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children [PubMed: PMC3694825] |
| Biomarker insights | Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) [PubMed: PMC3762604] |
| BMC cell biology | BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause [PubMed: PMC3640998] |
| Cell Journal (Yakhteh) | Effect of Mesenchymal Stem Cells on Doxorubicin-Induced Fibrosis [PubMed: PMC3584430] |
| Gastroenterol Res Pract | Proinflammatory Cytokines (IL-1α, IL-6) and Hepatocyte Growth Factor in Patients with Alcoholic Liver Cirrhosis [PubMed: 26448742] |
| Tumor Biology | Hypoxia-induced secretion of IL-11 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151] |
| Cytotherapy | Cell type–dependent variation in paracrine potency determines therapeutic efficacy against neonatal hyperoxic lung injury [PubMed: 25863963] |
| Biomedicine & Pharmacotherapy | Silymarin-loaded Eudragit? RS100 nanoparticles improved the ability of silymarin to resolve hepatic fibrosis in bile duct ligated rats [Pubmed:27261582] |
| Can J Physiol Pharmacol.? | Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis [Pubmed:27186801] |
| Genet Mol Res | Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064] |
| Changes in HGF levels after a progressive test before and after Tabata HIIT program [profile:Pawel_Wolanski3] | |
| Stem Cell Research & Therapy | Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats [10.1186/s13287-017-0739-3] |
| Oncology?Letters | Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c?Met/PI3K/AKT in vitro [10.3892/ol.2017.7301] |
| Stem Cell Research & Therapy | Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure. [pubmed:28679425] |
| Nature?Communications | Pulmonary pericytes regulate lung morphogenesis [Pubmed:29934496] |
| Experimental And Molecular?Medicine | Interleukin-1 receptor antagonist-mediated neuroprotection by umbilical cord-derived mesenchymal stromal cells following transplantation into a rodent stroke?… [Pubmed:29650950] |
| Oncology?Letters | Carcinoma associated fibroblasts derived from oral squamous cell carcinoma promote lymphangiogenesis via c-Met/PI3K/AKT in vitro [Pubmed:29375714] |
| Bulletin?of Experimental?Biology?and?Medicine | Dynamics of Expression of Cytokine Genes and Macrophage Content in the Lungs and Kidneys after Subtotal Hepatectomy in Rats [Pubmed:29796817] |
| Cell Biology International | Inherent control of hepatocyte proliferationInherent control of hepatocyte proliferation after subtotal liver resection [Pubmed: 31297922] |
| Journal of stem cells & regenerative medicine | What is the Best Degree of Hyaluronic Acid Crosslinking in Increasing Growth Factors Level of Platelet-Rich Fibrin Lysate? [Pubmed: 31239605] |
| Open Journal of Veterinary Medicine | Efficacy of autologous bone marrow mononuclear cell transplantation in dogs with chronic spinal cord injury [Pubmed: 32821665] |
| Stem Cell Reviews and Reports | Prospects on the Potential In Vitro Regenerative Features of Mechanically Treated-Adipose Tissue for Osteoarthritis Care [33469783] |
| Molecular Biology Reports | Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling?… [Pubmed:35585377] |
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