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10kDa干擾素γ誘導(dǎo)蛋白(IP10)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Interferon Gamma Induced Protein 10kDa (IP10)

CXCL10; C7; IFI10; INP10; SCYB10; Crg2; GIP10; Mob1; Chemokine C-X-C Motif Ligand 10; Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 10

  • 10kDa干擾素γ誘導(dǎo)蛋白(IP10)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 10kDa干擾素γ誘導(dǎo)蛋白(IP10)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)10kDa干擾素γ誘導(dǎo)蛋白(IP10)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過(guò)夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開(kāi)封,在4度保存時(shí)間為1個(gè)月。

配套試劑

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參考文獻(xiàn)

雜志 參考文獻(xiàn)
20 Melatonin inhibits type 1 interferon signaling of toll-like receptor 4 via heme oxygenase-1 induction in hepatic ischemia/reperfusion. [PubMed: 22288937]
BMC Pregnancy and Childbirth Maternal circulating leukocytes display early chemotactic responsiveness during late gestation [PubMed: PMC3561147]
Rheumatology International Serum levels of selected chemokines in systemic lupus erythematosus patients [Pubmed: 22461186]
PLOS ONE The Immunosuppressant Protosappanin A Diminished Recipient T Cell Migration into Allograft via Inhibition of IP-10 in Rat Heart Transplant [Pubmed:24798458]
Front Pharmacol The G-Protein-Coupled Bile Acid Receptor Gpbar1 (TGR5) Inhibits Gastric Inflammation Through Antagonizing NF-κB Signaling Pathway [PubMed: 26696888]
Cytokine Hypoxia/ischemia promotes CXCL10 expression in cardiac microvascular endothelial cells by NFkB activation [Pubmed:26891076]
Journal of Experimental & Clinical Cancer Research LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4. [pubmed:28641579]
Journal of Geriatric?Oncology Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors [Pubmed: 30952517]
Hepatology Integrated Omics Reveals Tollip as an Aggravator and Therapeutic Target for Hepatic Ischemia‐Reperfusion Injury in Mice [Pubmed: 31077413]
Molecular Medicine Reports IL?17A promotes CXCR2?dependent angiogenesis in a mouse model of liver cancer [Pubmed: 31173199]
JOURNAL OF MOLECULAR?NEUROSCIENCE Inflammatory Responses of Astrocytes Are Independent from Lipocalin 2 [Pubmed: 32959226]
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