白介素1α(IL1a)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Interleukin 1 Alpha (IL1a)
IL1-A; IL-1α; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1
- 編號(hào)KSA071Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍15.6-1,000pg/mL
- 可適靈敏度6.4pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 4752 ¥ 7920 ¥ 14256 ¥ 27720 ¥ 47520
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)白介素1α(IL1a)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA071Ra02 | 白介素1α(IL1a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA071Ra01 | 白介素1α(IL1a)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA071Ra02 | 白介素1α(IL1a)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA071Ra01 | 白介素1α(IL1a)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA071Ra71 | 白介素1α(IL1a)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA071Ra22 | 白介素1α(IL1a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA071Ra21 | 白介素1α(IL1a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA071Ra23 | 白介素1α(IL1a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA071Ra24 | 白介素1α(IL1a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA071Ra25 | 白介素1α(IL1a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA071Ra27 | 白介素1α(IL1a)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA071Ra29 | 白介素1α(IL1a)單克隆抗體 | WB; IHC; ICC; IP. |
| HEA071Ra | 白介素1α(IL1a)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA071Ra | 白介素1α(IL1a)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA071Ra | 白介素1α(IL1a)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA071Ra01 | 白介素1α(IL1a)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| The Veterinary Journal | Quantitative assessment of hsp70, IL-1β and TNF-α in the spinal cord of dogs with E40K SOD1-associated degenerative myelopathy [Pubmed: 24662024] |
| Int J Clin Exp Pathol | Expression of S100 family proteins in neonatal rats with sepsis and its significance [PubMed: 25973048] |
| Tumor Biology | Hypoxia-induced secretion of IL-17 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151] |
| Parasites?&?Vectors | Vaccination?of?rabbits?with?immunodominant?antigens?from?Sarcoptes?scabiei?induced?highlevels?of?humoral?responses?and?pro-inflammatory?cytokines but confers limited protection. [pubmed:27502394] |
| Cell Death Dis. | Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy [pubmed:28333148] |
| Journal of Cosmet Dermatol. | Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties [DOI: 10.1111/jocd.12349] |
| Scientific Reports | Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets [pubmed:28811665] |
| Arthritis Research and Therapy | Increased synovial lipodystrophy induced by high fat diet aggravates synovitis in experimental osteoarthritis. [pubmed:29191221] |
| International Journal of Molecular Sciences | Screening of circular RNAs and validation of circANKRD36 associated with inflammation in patients with type 2 diabetes mellitus [Pubmed:30066828] |
| Journal of Molecular Neuroscience | Neuroprotective effect of curcumin against cerebral ischemia-reperfusion via mediating autophagy and inflammation [Pubmed:29243061] |
| Journal of?Maternal-Fetal?&?Neonatal?Medicine | Amniotic fluid HIF1α and exosomal HIF1α in cervical insufficiency patients with physical examination-indicated cerclage [Pubmed:29357727] |
| Brazilian Dental Journal | Radiographic and Immunohistochemical Evaluation of Root Canal Treatment Using Different Irrigation Systems [Pubmed: 30970054] |
| The role of amniotic fluid exosome, hypoxia inducible fator-1α, and inflammatory cytokine in cervical insufficiency [] | |
| OPEN ACCESS MACEDONIAN JOURNAL OF MEDICAL SCIENCES | Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus [] |
| Polish Journal of Veterinary Sciences | Effect of postpartum administration of ketoprofen on proinflammatory cytokine concentration and their correlation with lipogenesis and ketogenesis in Holstein?… [Pubmed: 31560476] |
| Nanomedicine | The toxicity of alumina nanoparticles to immune system in mice [Pubmed: 32162999] |
| Theranostics | Biomechanically, structurally and functionally meticulously tailored polycaprolactone/silk fibroin scaffold for meniscus regeneration [Pubmed: 32308770] |
| STEM CELLS AND DEVELOPMENT | IL-1α regulates osteogenesis and osteoclastic activity of dental follicle cells via JNK and p38 MAPK pathways [Pubmed: 33107399] |
| FASEB JOURNAL | ANXA1 directs Schwann cells proliferation and migration to accelerate nerve regeneration through the FPR2/AMPK pathway [Pubmed: 32908567] |
| BIOLOGICAL TRACE ELEMENT RESEARCH | Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle [Pubmed: 32583225] |
| Cancer Lett | Photodynamic therapy induces human esophageal carcinoma cell pyroptosis by targeting the PKM2/caspase-8/caspase-3/GSDME axis [34256094] |
| Brain Res | Baicalin suppresses neuron autophagy and apoptosis by regulating astrocyte polarization in pentylenetetrazol-induced epileptic rats and PC12 cells [34780748] |
| Animals (Basel) | Inflammatory markers in uterine lavage fluids of pregnant, non-pregnant, and intrauterine device implanted mares on Days 10 and 15 post ovulation [34944269] |
| Animals (Basel) | Inflammatory Markers in Uterine Lavage Fluids of Pregnant, Non-Pregnant, and Intrauterine Device Implanted Mares on Days 10 and 15 Post Ovulation?… [Pubmed:34944269] |
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