異檸檬酸脫氫酶1(IDH1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Isocitrate Dehydrogenase 1, Soluble (IDH1)
PICD; IDP; Oxalosuccinate decarboxylase; NADP(+)-specific ICDH; Cytosolic NADP-isocitrate dehydrogenase
- 編號KSH839Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.781-50ng/mL
- 可適靈敏度0.37ng/mL
- 應用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
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特異性
試劑盒中的抗體對異檸檬酸脫氫酶1(IDH1)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
RPH839Hu01 | 異檸檬酸脫氫酶1(IDH1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAH839Hu01 | 異檸檬酸脫氫酶1(IDH1)多克隆抗體 | WB,IHC |
FAH839Hu01 | 抗異檸檬酸脫氫酶1(IDH1)多克隆抗體 | Flow cytometry. |
MAH839Hu22 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體 | WB; IHC |
MAH839Hu23 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體 | WB; IHC; ICC; IP. |
MAH839Hu25 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體 | WB; IHC; ICC; IP. |
MAH839Hu27 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體 | WB; IHC; ICC; IP. |
MAH839Hu29 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體 | WB; IHC; ICC; IP. |
MAH839Hu24 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體 | WB; IHC |
MAH839Hu26 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體 | WB; IHC |
MAH839Hu21 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體 | WB; IHC; ICC; IP. |
RAH839Hu21 | 異檸檬酸脫氫酶1(IDH1)重組抗體 | WB; IFC; IHC; IP. |
FAH839Hu02 | 抗異檸檬酸脫氫酶1(IDH1)單克隆抗體 | Flow cytometry. |
FAH839Hu04 | 抗異檸檬酸脫氫酶1(IDH1)單克隆抗體 | Flow cytometry. |
FAH839Hu06 | 抗異檸檬酸脫氫酶1(IDH1)單克隆抗體 | Flow cytometry. |
FAH839Hu08 | 抗異檸檬酸脫氫酶1(IDH1)單克隆抗體 | Flow cytometry. |
LAH839Hu76 | 異檸檬酸脫氫酶1(IDH1)單克隆抗體(生物素標記) | WB; IHC; ICC. |
IEH839Hu | 異檸檬酸脫氫酶1(IDH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEH839Hu | 異檸檬酸脫氫酶1(IDH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCH839Hu | 異檸檬酸脫氫酶1(IDH1)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMH839Hu | 異檸檬酸脫氫酶1(IDH1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
PSH839Hu01 | 異檸檬酸脫氫酶1(IDH1)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSH839Hu01 | 異檸檬酸脫氫酶1(IDH1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
Molecular & Cellular Proteomics | Identification of IDH1 as a Potential Diagnostic and Prognostic Biomarker for Non-small-cell Lung Cancer by Proteomic Analysis [Mcponline: 008821] |
Molecular & Cellular Proteomics | Identification of Isocitrate Dehydrogenase 1 as a Potential Diagnostic and Prognostic Biomarker for Non-small Cell Lung Cancer by Proteomic Analysis [Pubmed: 22064513] |
Clinical Cancer Research | Isocitrate dehydrogenase 1 is a novel plasma biomarker for the diagnosis of non-small cell lung cancer [Pubmed: 24046070] |
Onco Targets Ther | The effects of PGC-1α on the proliferation and energy metabolism of malignant endometrial cancer cells [PubMed: 25914546] |
Oncogene | Metformin sensitizes endometrial cancer cells to chemotherapy through IDH1-induced Nrf2 expression via an epigenetic mechanism [Pubmed:29921847] |
Molecular Oncology | IDH 2 is a novel diagnostic and prognostic serum biomarker for non‐small‐cell lung cancer [Pubmed:29465809] |
Cancers | Serological Biomarkers for Early Detection of Hepatocellular Carcinoma: A Focus on Autoantibodies against Tumor-Associated Antigens Encoded by Cancer Driver … [Pubmed: 32443439] |
International Journal of Cancer | Proteome profiling of enzalutamide‐resistant cell lines and serum analysis identified ALCAM as marker of resistance in castration‐resistant prostate cancer [Pubmed:35689436] |
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