瘦素(LEP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Leptin (LEP)
OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein
- 編號(hào)KSA084Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.156-10ng/mL
- 可適靈敏度0.058ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 5054 ¥ 8424 ¥ 15163 ¥ 29484 ¥ 50544
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特異性
試劑盒中的抗體對(duì)瘦素(LEP)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
APA084Hu01 | 瘦素(LEP)活性蛋白 | Cell?culture;?Activity?Assays. |
RPA084Hu01 | 瘦素(LEP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA084Hu01 | 瘦素(LEP)多克隆抗體 | WB; IHC; ICC; IP. |
LAA084Hu71 | 瘦素(LEP)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
LAA084Hu81 | 瘦素(LEP)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
MAA084Hu22 | 瘦素(LEP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA084Hu21 | 瘦素(LEP)單克隆抗體 | WB |
MAA084Hu23 | 瘦素(LEP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA084Hu24 | 瘦素(LEP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA084Hu25 | 瘦素(LEP)單克隆抗體 | WB; IHC; ICC; IP. |
HEA084Hu | 瘦素(LEP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEA084Hu | 瘦素(LEP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA084Hu | 瘦素(LEP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA084Hu | 瘦素(LEP)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA084Hu | 瘦素(LEP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA084Hu01 | 瘦素(LEP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
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