粘蛋白5AC(MUC5AC)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Mucin 5 Subtype AC (MUC5AC)
TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein
- 編號(hào)KSA756Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍78-5,000pg/mL
- 可適靈敏度34pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 6804 ¥ 11340 ¥ 20412 ¥ 39690 ¥ 68040
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)粘蛋白5AC(MUC5AC)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA756Hu01 | 粘蛋白5AC(MUC5AC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA756Hu01 | 粘蛋白5AC(MUC5AC)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA756Hu81 | 粘蛋白5AC(MUC5AC)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAA756Hu71 | 粘蛋白5AC(MUC5AC)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA756Hu21 | 粘蛋白5AC(MUC5AC)單克隆抗體 | WB |
| MAA756Hu22 | 粘蛋白5AC(MUC5AC)單克隆抗體 | WB; IHC; ICC; IP. |
| LAA756Hu72 | 粘蛋白5AC(MUC5AC)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEA756Hu | 粘蛋白5AC(MUC5AC)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA756Hu | 粘蛋白5AC(MUC5AC)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA756Hu | 粘蛋白5AC(MUC5AC)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSA756Hu01 | 粘蛋白5AC(MUC5AC)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA756Hu01 | 粘蛋白5AC(MUC5AC)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Cell & Molecular Biology | Role of aquaporin 5 in antigen-induced airway inflammation and mucous hyperproduction in mice [PubMed: 20550619] |
| Mucosal Immunology | Homeostatic control of conjunctival mucosal goblet cells by NKT-derived IL-13 [Nature: 201082a] |
| European Journal of Pharmacology | Naringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs-AP-1 and IKKs-IκB-NF-κB signaling pathways [ScienceDirect: S0014299912005523] |
| PLoS One | Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation [PubMed: PMC3639905] |
| Respiratory Researh | Pyocyanin-induced mucin production is associated with redox modification of FOXA2 [PubMed: PMC3765780] |
| Surgery | A novel serum marker for biliary tract cancer: Diagnostic and prognostic values of quantitative evaluation of serum mucin 5AC (MUC5AC) [Pubmed: 24468034] |
| Biochemical and Biophysical Research Communications | The human cathelicidin LL-37 enhances airway mucus production in chronic obstructive pulmonary disease [Pubmed:24291709] |
| Journal of Proteomics | Proteomic analysis of cancer and mesothelial cells reveals an increase in Mucin 5AC during ovarian cancer and peritoneal interaction [Pubmed:24726482] |
| Experimental Lung Research | The human Cathelicidin LL-37 induces MUC5AC mucin production by airway epithelial cells via TACE-TGF-α-EGFR pathway [Pubmed:24901072] |
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| Respirology | Secreted mucins and airway bacterial colonization in non-CF bronchiectasis [PubMed: 26172851] |
| S | Isabela Casagrande Jeremias [Teses: Disponiveis] |
| American Thoracic Society International Conference Abstracts | Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsiveness [doi:10.1164] |
| Deletion Polymorphism and Chronic Obstructive Pulmonary Disease | ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial Cells [Pubmed:27075970] |
| Int J Clin Exp Med | The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghai [files:ijcem0020163.pdf] |
| Ann Am Thorac Soc | Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization [doi:10.1513] |
| Sci Rep | Impact of Cigarette Smoking on Tear Function and Correlation between Conjunctival Goblet Cells and Tear MUC5AC Concentration in Office Workers [Pubmed:27297822] |
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| J Virol.? | Mutating the CX3C Motif in the G Protein Should Make a Live Respiratory Syncytial Virus Vaccine Safer and More Effective. [pubmed:28275196] |
| Respiratory Physiology & Neurobiology | Peroxiredoxin 6 suppresses Muc5ac overproduction in LPS-induced airway inflammation through H2O2-EGFR-MAPK signaling pathway. [pubmed:27884794] |
| Journal of Immunology Research | Beneficial effects of prebiotic Saccharomyces cerevisiae mannan on allergic asthma mouse models [pubmed:28835901] |
| BASIC INVESTIGATION | Efficacy of Sodium Hyaluronate in Murine Diabetic Ocular Surface Diseases [pubmed:28644234] |
| International Immunopharmacology | Clarithromycin inhibits TNF-α-induced MUC5AC mucin gene expression via the MKP-1-p38MAPK-dependent pathway [pubmed:28550735] |
| Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой [10.20538/1682-0363-2017-2-146-158] | |
| Cellular Microbiology | An adherent mucus layer attenuates the genotoxic effect of colibactin. [pubmed:29156489] |
| Respiratory Research | IL-4-induced caveolin-1-containing lipid rafts aggregation contributes to MUC5AC synthesis in bronchial epithelial cells [pubmed:28931396] |
| Chemotherapy | Oxytetracycline Inhibits Mucus Secretion and Inflammation in Human Airway Epithelial Cells. [pubmed:28578323] |
| Allergy | Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polyps [Pubmed:29802623] |
| European?Journal of Pharmacology? | Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling [Pubmed:29382534] |
| Inflammation | Plantamajoside Inhibits Lipopolysaccharide-Induced MUC5AC Expression and Inflammation through Suppressing the PI3K/Akt and NF-κB Signaling Pathways in?… [Pubmed:29349683] |
| Pulmonary Pharmacology and Therapeutics | In vivo and in vitro investigation of anti-inflammatory and mucus-regulatory activities of a fixed combination of thyme and primula extracts [Pubmed:29698781] |
| European Respiratory Society | Profiling of healthy and asthmatic airway smooth muscle cells following IL-1β treatment: a novel role for CCL20 in chronic mucus hyper-secretion [Pubmed:29946002] |
| Clinical?and?Experimental?Ophthalmology | Development of a highly sensitive and reliable enzyme-linked immunosorbent assay for MUC5aC in human tears extracted from schirmer strips [Pubmed: 30214141] |
| international journal of biological macromolecules | Increased expression of long non-coding RNA SNHG16 correlates with tumor progression and poor prognosis in non-small cell lung cancer [Pubmed: 30287374] |
| Journal of Investigative?Medicine | The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease [Pubmed: 30573494] |
| Phytomedicine | Regulation effects of naringin on diesel particulate matter-induced abnormal airway surface liquid secretion [Pubmed: 31301536] |
| Scientific Reports | Regional comparison of Goblet cell number and Area in exposed and covered Dry eyes and their Correlation with Tear MUC5AC [Pubmed: 32076085] |
| Eur J Pharmacol | TLR3 inhibitor and tyrosine kinase inhibitor attenuate cigarette smoke/poly I: C-induced airway inflammation and remodeling by the EGFR/TLR3/MAPK?… [Pubmed: 33068589] |
| journal of ethnopharmacology | Effective-component compatibility of Bufei Yishen formula II inhibits mucus hypersecretion of chronic obstructive pulmonary disease rats by regulating EGFR/PI3K … [Pubmed: 32344236] |
| Less Airway Inflammation and Goblet Cell Metaplasia in an IL-33-Induced Asthma Model of Leptin-Deficient Obese Mice [] | |
| Exp Mol Pathol | The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal??- [33285209] |
| FEBS Open Bio | Porphyromonas gingivalis gingipains potentially affect MUC5AC gene expression and protein levels in respiratory epithelial cells [33332733] |
| Cell Rep | Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs [33535050] |
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