粘蛋白5B(MUC5B)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Mucin 5 Subtype B (MUC5B)
MUC5-B; MUC9; MG1; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtype B,Tracheobronchial; Cervical mucin; High molecular weight salivary mucin MG1; Sublingual gland mucin
- 編號(hào)KSA684Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱(chēng)，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.625-40ng/mL
- 可適靈敏度0.255ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 6221 ¥ 10368 ¥ 18662 ¥ 36288 ¥ 62208
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)粘蛋白5B(MUC5B)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA684Mu02 | 粘蛋白5B(MUC5B)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA684Mu01 | 粘蛋白5B(MUC5B)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA684Mu01 | 粘蛋白5B(MUC5B)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA684Mu02 | 粘蛋白5B(MUC5B)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA684Mu81 | 粘蛋白5B(MUC5B)多克隆抗體(異硫氰酸熒光素標(biāo)記) | Applications: WB; IHC; ICC; IP. |
| LAA684Mu71 | 粘蛋白5B(MUC5B)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEA684Mu | 粘蛋白5B(MUC5B)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA684Mu | 粘蛋白5B(MUC5B)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA684Mu01 | 粘蛋白5B(MUC5B)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| The Journal of Biological Chemistry | The Extracellular Signal-regulated Kinase Mitogen-activated Protein Kinase/Ribosomal S6 Protein Kinase 1 Cascade Phosphorylates cAMP Response Element-binding Protein to Induce MUC5B Gene Expression via D-Prostanoid Receptor Signaling [Jcb: 34199] |
| The Journal of Biological Chemistry | The ERK MAPK/RSK1 cascade phosphorylates CREB to induce MUC5B gene expression via DP1 receptor signaling [Jcb: 247684] |
| Respiratory Researh | Pyocyanin-induced mucin production is associated with redox modification of FOXA2 [PubMed: PMC3765780] |
| Respirology | Secreted mucins and airway bacterial colonization in non-CF bronchiectasis [PubMed: 26172851] |
| American Thoracic Society International Conference Abstracts | Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsiveness [doi:10.1164] |
| Ann Am Thorac Soc | Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization [doi:10.1513] |
| Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой [10.20538/1682-0363-2017-2-146-158] | |
| Allergy | Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polyps [Pubmed:29802623] |
| European?Journal of Pharmacology? | Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling [Pubmed:29382534] |
| Japan Society of Immunology & Allergology in Otolaryngology | インフルエンザ菌Phosphorylcholine の表出と中耳ムチンMUC5AC 及びMUC5B 産生への影響 [10.5648:jjiao.36.7] |
| Journal of Investigative?Medicine | The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease [Pubmed: 30573494] |
| Mucosal Immunology | ASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanism [Pubmed: 31278375] |
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