脂質(zhì)運(yùn)載蛋白2(NGAL)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Neutrophil gelatinase-associated lipocalin (NGAL)
Lipocalin-2; LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2
- 編號(hào)KSB388Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.156-10ng/mL
- 可適靈敏度0.055ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 4838 ¥ 8064 ¥ 14515 ¥ 28224 ¥ 48384
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產(chǎn)品包裝(模擬)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)中性粒細(xì)胞明膠酶關(guān)聯(lián)脂質(zhì)運(yùn)載蛋白(NGAL)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APB388Mu01 | 脂質(zhì)運(yùn)載蛋白2(NGAL)活性蛋白 | Cell?culture;?Activity?Assays. |
| APB388Mu61 | 脂質(zhì)運(yùn)載蛋白2(NGAL)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB388Mu02 | 脂質(zhì)運(yùn)載蛋白2(NGAL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB388Mu61 | 脂質(zhì)運(yùn)載蛋白2(NGAL)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB388Mu01 | 脂質(zhì)運(yùn)載蛋白2(NGAL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB388Mu01 | 脂質(zhì)運(yùn)載蛋白2(NGAL)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB388Mu02 | 脂質(zhì)運(yùn)載蛋白2(NGAL)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB388Mu81 | 脂質(zhì)運(yùn)載蛋白2(NGAL)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAB388Mu71 | 脂質(zhì)運(yùn)載蛋白2(NGAL)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEB388Mu | 脂質(zhì)運(yùn)載蛋白2(NGAL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB388Mu | 脂質(zhì)運(yùn)載蛋白2(NGAL)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB388Mu | 脂質(zhì)運(yùn)載蛋白2(NGAL)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB388Mu01 | 脂質(zhì)運(yùn)載蛋白2(NGAL)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Asian Journal of Sports Medicine | Effects of Resistance versus Endurance Training on Plasma Lipocalin-2 in Young Men [Tums: Source] |
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| Diabetes & Vascular Disease Research | Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats [Sagepub:Source] |
| Biomedical & Pharmacology Journal | Hs-CRP and Adipokin (Lcn2): Response to Exercise Training in Obese Men [Biomedpharmajournal:Source] |
| Asian Journal of Physical Education and Computer Science in Sports | A Study On Thyroid Hormone And Its Effects On Exercise [Ifcss:Source] |
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| Archives of Biological Sciences | Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol [publication:299492265_Diabetes-induced_renal_failu] |
| International Journal of Molecular Medicine | The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats [pubmed:27430552] |
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| Molecules | Effect of Pinocembrin Isolated from Mexican Brown Propolis on Diabetic Nephropathy [Pubmed:29642511] |
| American Journal of Translational?Research | Improved approach for normothermic machine perfusion of cold stored kidney grafts [Pubmed:30018731] |
| Chronic obstructive pulmonary disease (COPD)--from biomarkers to clinical phenotypes [ISBN:978-951-51-4039-5] | |
| Disease Markers | Potential Novel Biomarkers of Obstructive Nephropathy in Children with Hydronephrosis [Doi: 10.1155/2018/1015726] |
| Kidney?and?Blood?Pressure?Research | The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy [Pubmed: 30504713] |
| BMC?Complementary and Alternative Medicine | Dual actions on gout flare and acute kidney injury along with enhanced renal transporter activities by Yokuininto, a Kampo medicine [Pubmed: 30871515] |
| Naunyn-Schmiedeberg's archives of pharmacology | Piceatannol protects against cisplatin nephrotoxicity via activation of Nrf2/HO-1 pathway and hindering NF-κB inflammatory cascade [Pubmed: 31197431] |
| Diabetes, Metabolic Syndrome and Obesity | A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation [Pubmed: 31447572] |
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| Scientific Reports | Alkaline phosphatase treatment of Acute Kidney injury in an infant piglet Model of cardiopulmonary Bypass with Deep Hypothermic circulatory Arrest [Pubmed: 31578351] |
| Int Urol Nephrol | The evaluation of acute kidney injury due to ischemia by urinary neutrophil gelatinase-induced lipocalin (uNGAL) measurement in patients who underwent partial?… [Pubmed: 32980930] |
| human & experimental toxicology | Upregulated miR-101 inhibits acute kidney injury–chronic kidney disease transition by regulating epithelial–mesenchymal transition [Pubmed: 32633566] |
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| INTERNATIONAL JOURNAL OF ENDOCRINOLOGY | Thyroid function in patients with type 1 diabetes mellitus and chronic kidney disease receiving renal replacement therapy [] |
| Pediatr Int | Early Kidney Injury in Immunoglobulin A Vasculitis: Role of Renal Biomarkers [33423326] |
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| Biomed Res Int | Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury [33604384] |
| International Journal of Medical Sciences | Elevated plasma level of neutrophil gelatinase-associated lipocalin (NGAL) in patients with breast cancer [34104101] |
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